Avi Tag Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB10546-100
MAB10546-SP
Detection of Avi Tag by Western Blot.
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Avi Tag Antibody Summary

Specificity
Detects proteins with Avi Tag in direct ELISAs and Western blots. In Western blots, no cross-reactivity with proteins with His Tag is observed.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2623F
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human OX40/TNFRSF4 Avi-tag
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant human VEGF-165 Avi Tag protein
Neutralization

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Avi Tag antibody by Western Blot. View Larger

Detection of Avi Tag by Western Blot. Western blot shows recombinant human VEGF-165 Avi Tag protein and recombinant human OX40 His Tag protein (negative control). PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Avi Tag Monoclonal Antibody (Catalog # MAB10546) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for recombinant human VEGF-165 Avi Tag protein at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western Detection of Avi Tag antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Avi Tag by Simple WesternTM. Simple Western lane view shows recombinant OX40 Avi Tag protein, loaded at 0.2 mg/mL. A specific band was detected for recombinant OX40 Avi Tag at approximately 74 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Avi Tag Monoclonal Antibody (Catalog # MAB10546). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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