Avi Tag Antibody (2623F)

R&D Systems | Catalog # MAB10546

Recombinant Monoclonal Antibody.
R&D Systems

Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, Neutralization

Cited:

Chromatin Immunoprecipitation (ChIP), ELISA Capture, Surface Plasmon Resonance (SPR)

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2623F
View all Avi Tag Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human OX40/TNFRSF4 Avi-tag

Specificity

Detects proteins with Avi Tag in direct ELISAs and Western blots. In Western blots, no cross-reactivity with proteins with His Tag is observed.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Avi Tag Antibody (2623F)

Detection of Avi Tag antibody by Western Blot.

Detection of Avi Tag by Western Blot.

Western blot shows recombinant human VEGF-165 Avi Tag protein and recombinant human OX40 His Tag protein (negative control). PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Avi Tag Monoclonal Antibody (Catalog # MAB10546) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for recombinant human VEGF-165 Avi Tag protein at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of Avi Tag antibody by Simple WesternTM.

Detection of Avi Tag by Simple WesternTM.

Simple Western lane view shows recombinant OX40 Avi Tag protein, loaded at 0.2 mg/mL. A specific band was detected for recombinant OX40 Avi Tag at approximately 74 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Avi Tag Monoclonal Antibody (Catalog # MAB10546). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Avi Tag Antibody (2623F)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant human VEGF-165 Avi Tag protein

Neutralization

20 µg/mL

Reviewed Applications

Read 1 review rated 5 using MAB10546 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Avi Tag

Alternate Names

Avi Epitope Tag, AviTag

Additional Avi Tag Products

Product Documents for Avi Tag Antibody (2623F)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Avi Tag Antibody (2623F)

For research use only

Citations for Avi Tag Antibody (2623F)

Customer Reviews for Avi Tag Antibody (2623F) (1)

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  • Avi Tag Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Adult brain
    Species: Mouse
    Verified Customer | Posted 06/23/2023
    Avi Tag Antibody (2623F) MAB10546

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