B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-80588
Key Product Details
Species Reactivity
Human, Mouse
Applications
Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 62N3G8
Format
Azide and BSA Free
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Product Specifications
Immunogen
Amino acids 102-223 of human CD80 were used as the immunogen for the antibody.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 27440778).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
Novus Biologicals Mouse B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free (NBP2-25255) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free
Detection of B7-1/CD80 in Human Hodgkin's Lymphoma via seqIF™ staining on COMET™
B7-1/CD80 was detected in immersion fixed paraffin-embedded sections of human Hodgkin's Lymphoma using Mouse Anti-Human B7-1/CD80, Monoclonal Antibody at 20ug/mL at 37°Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.
![Western Blot: B7-1/CD80 Antibody (62N3G8)Azide and BSA Free [NBP2-80588]](https://beta-resources.rndsystems.com/images/products/B7-1-CD80-Antibody-62N3G8-Azide-and-BSA-Free-Western-Blot-NBP2-80588-img0002.jpg "Western Blot: B7-1/CD80 Antibody (62N3G8)Azide and BSA Free [NBP2-80588]")
Western Blot: B7-1/CD80 Antibody (62N3G8)Azide and BSA Free [NBP2-80588]
Western Blot: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588] - Analysis of CD80 in A) a partial recombinant protein and B) Daudi lysate using this antibody at 0.1 and 1 g/ml, respectively. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test. Image from the standard format of this antibody.![Immunocytochemistry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588]](https://beta-resources.rndsystems.com/images/products/B7-1-CD80-Antibody-62N3G8-Azide-and-BSA-Free-Immunocytochemistry-NBP2-80588-img0003.jpg "Immunocytochemistry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588]")
Immunocytochemistry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588]
Immunocytochemistry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588] - Human thymus stained with CD80 antibody at 5 ug/ml using peroxidase-conjugate and DAB chromogen. Note membrane and cytoplasmic staining. Image from the standard format of this antibody.![Flow Cytometry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588]](https://beta-resources.rndsystems.com/images/products/B7-1-CD80-Antibody-62N3G8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80588-img0004.jpg "Flow Cytometry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588]")
Flow Cytometry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588]
Flow Cytometry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-25255. Staining of CD80 in human PBMC (monocyte depleted) using anti-CD80 antibody. Image from verified customer review. Image from the standard format of this antibody.![Western Blot: B7-1/CD80 Antibody (62N3G8)Azide and BSA Free [NBP2-80588]](https://beta-resources.rndsystems.com/images/products/B7-1-CD80-Antibody-62N3G8-Azide-and-BSA-Free-Western-Blot-NBP2-80588-img0001.jpg "Western Blot: B7-1/CD80 Antibody (62N3G8)Azide and BSA Free [NBP2-80588]")
Western Blot: B7-1/CD80 Antibody (62N3G8)Azide and BSA Free [NBP2-80588]
Western Blot: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588] - Analysis in human Raji lymphoma cells. Image from the standard format of this antibody.![Flow Cytometry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588]](https://beta-resources.rndsystems.com/images/products/B7-1-CD80-Antibody-62N3G8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80588-img0005.jpg "Flow Cytometry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588]")
Flow Cytometry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588]
Flow Cytometry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588] - Raji lymphoma cells. Image from verified customer review. Image from the standard format of this antibody.Applications for B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000. Use reported by customer review
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
reported by customer review
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: B7-1/CD80
Alternate Names
B71, CD80
Gene Symbol
CD80
Additional B7-1/CD80 Products
Product Documents for B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free
Product Specific Notices for B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
