Bcl-2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-30108

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Chicken (93%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Bcl-2 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 15 amino acids near the N terminus of human Bcl-2. The immunogen is located within the first 50 amino acids of Bcl-2. Amino Acid Squence: KYIHYKLSQRGYEWD

Specificity

Human Bcl-2 has 2 isoforms, including isoform 1 (239aa, 26kD), and isoform 2 (205aa, 22kD). Mouse Bcl-2 has 2 isoforms, including isoform 1 (236aa, 26kD), and isoform 2 (199aa, 22kD). Rat Bcl-2 has one isoform (236aa, 27kD). NBP2-30108 can detect all isoforms of human, mouse and rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Bcl-2 Antibody - BSA Free

Western Blot: Bcl-2 AntibodyBSA Free [NBP2-30108]

Western Blot: Bcl-2 AntibodyBSA Free [NBP2-30108]

Western Blot: Bcl-2 Antibody [NBP2-30108] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane.Antibodies: Bcl-2 NBP2-30108, (2 ug/mL), Bcl-2 from Company B, (2 ug/mL), beta-actin (1 ug/mL) and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Bcl-2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Bcl-2 Antibody - BSA Free [NBP2-30108] -

Immunocytochemistry/ Immunofluorescence: Bcl-2 Antibody - BSA Free [NBP2-30108] - Figure 7 Immunofluorescence Validation of Bcl-2 in Mouse Brain TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed Mouse Brain Tissue labeling Bcl-2 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).
Immunohistochemistry-Paraffin: Bcl-2 Antibody - BSA Free [NBP2-30108]

Immunohistochemistry-Paraffin: Bcl-2 Antibody - BSA Free [NBP2-30108]

Immunohistochemistry-Paraffin: Bcl-2 Antibody [NBP2-30108] - Immunohistochemistry Validation of Bcl-2 in Human Kidney. Immunohistochemical analysis of paraffin-embedded Human Kidney using anti-Bcl-2 antibody (NBP2-30108) at 2ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Western Blot: Bcl-2 AntibodyBSA Free [NBP2-30108]

Western Blot: Bcl-2 AntibodyBSA Free [NBP2-30108]

Western Blot: Bcl-2 Antibody [NBP2-30108] - Western Blot Validation in Human and Mouse Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: Bcl-2 NBP2-30108, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane A: Human Daudi cellsLane B: Mouse A-20 cells
Western Blot: Bcl-2 AntibodyBSA Free [NBP2-30108]

Western Blot: Bcl-2 AntibodyBSA Free [NBP2-30108]

Western Blot: Bcl-2 Antibody [NBP2-30108] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: Bcl-2 NBP2-30108, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Bcl-2 Antibody - BSA Free

Immunohistochemistry: Bcl-2 Antibody - BSA Free [NBP2-30108] -

Immunohistochemistry: Bcl-2 Antibody - BSA Free [NBP2-30108] - Figure 6 Immunohistochemistry Validation of Bcl-2 in Mouse Brain Tissue
Immunohistochemical analysis of paraffin-embedded Mouse Brain Tissue using anti-Bcl-2 antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Bcl-2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Bcl-2 Antibody - BSA Free [NBP2-30108] -

Immunocytochemistry/ Immunofluorescence: Bcl-2 Antibody - BSA Free [NBP2-30108] - Validation of Bcl-2 in Human Kidney Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Kidney Cells labeling Bcl-2 with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Applications for Bcl-2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10ug/ml

Immunohistochemistry-Paraffin

2ug/ml

Western Blot

1-2ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bcl-2

cl-2 Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Bcl-2 is the founding member of a family of over 20 proteins that are critical regulators of apoptosis. These can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bcl-2 is an inner mitochondrial membrane protein that inhibits apoptosis. It is thought to act by interacting with pro-apoptotic Bcl-2 family members such as Bak and Bad. Overexpression of Bcl-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.

Long Name

B Cell Lymphoma/Leukemia 2

Alternate Names

Bcl2

Gene Symbol

BCL2

UniProt

AAH27258 (Human)

Additional Bcl-2 Products

Product Documents for Bcl-2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Bcl-2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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