beta-Catenin Antibody (GT2169) - Azide and BSA Free

Name: beta-Catenin Antibody (GT2169) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP3-13530
Price: 489 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-13530

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (99%), Canine (99%), Chicken (97%), Porcine (99%), Rat (100%), Rhesus Macaque (100%), Sheep (99%), Xenopus (97%), Zebrafish (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # GT2169

Format

Azide and BSA Free

View all beta-Catenin Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human beta-Catenin. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Description

Centrifuge briefly prior to opening.

Scientific Data Images for beta-Catenin Antibody (GT2169) - Azide and BSA Free

Western Blot: beta-Catenin Antibody (GT2169) [NBP3-13530]

Western Blot: beta-Catenin Antibody (GT2169) [NBP3-13530] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Catenin antibody [GT2169] (NBP3-13530) diluted at 1:500.

Western Blot: beta-Catenin Antibody (GT2169) [NBP3-13530]

Western Blot: beta-Catenin Antibody (GT2169) [NBP3-13530] - Wild-type (WT) and beta-Catenin knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Catenin antibody [GT2169] (NBP3-13530) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Western Blot: beta-Catenin Antibody (GT2169) [NBP3-13530] -

Western Blot: beta-Catenin Antibody (GT2169) [NBP3-13530] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Catenin antibody (NBP3-13530) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (GT2169) [NBP3-13530] -

Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (GT2169) [NBP3-13530] - beta-Catenin antibody detects beta-Catenin protein at cell membrane by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta-Catenin stained by beta-Catenin antibody (NBP3-13530) diluted at 1:500.

Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (GT2169) [NBP3-13530] -

beta Catenin antibody [GT2169] detects beta Catenin protein at cell membrane by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta Catenin protein stained by beta Catenin antibody [GT2169] (NBP3-13530) diluted at 1:200.
Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: beta-Catenin Antibody (GT2169) [NBP3-13530] -

beta Catenin antibody [GT2169] detects beta Catenin protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
beta Catenin stained by beta Catenin antibody [GT2169] (NBP3-13530) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: beta-Catenin Antibody (GT2169) [NBP3-13530] -

beta Catenin antibody [GT2169] detects beta Catenin protein by immunohistochemical analysis.
Sample: Paraffin-embedded human pancreas.
beta Catenin stained by beta Catenin antibody [GT2169] (NBP3-13530) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: beta-Catenin Antibody (GT2169) [NBP3-13530] -

beta Catenin antibody [GT2169] detects beta Catenin protein by immunohistochemical analysis.
Sample: Paraffin-embedded human appendix.
beta Catenin stained by beta Catenin antibody [GT2169] (NBP3-13530) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: beta-Catenin Antibody (GT2169) [NBP3-13530] -

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [GT2169] (NBP3-13530) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

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Applications for beta-Catenin Antibody (GT2169) - Azide and BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: beta-Catenin

The protein encoded by the beta Catenin gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs arenecessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion betweencells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contactinhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this proteinbinds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this geneare a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.

Alternate Names

bCatenin, CTNNB1

Gene Symbol

CTNNB1

Additional beta-Catenin Products

Product Documents for beta-Catenin Antibody (GT2169) - Azide and BSA Free

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Certificate of Analysis

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Product Specific Notices for beta-Catenin Antibody (GT2169) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.