Brg1 Antibody (GT2712) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13537

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GT2712

Format

Azide and BSA Free
View all Brg1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Brg1. The exact sequence is proprietary.

Reactivity Notes

Chicken (84%), Bovine (87%)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

185 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Brg1 Antibody (GT2712) - Azide and BSA Free

Western Blot: Brg1 Antibody (GT2712) [NBP3-13537]

Western Blot: Brg1 Antibody (GT2712) [NBP3-13537]

Western Blot: Brg1 Antibody (GT2712) [NBP3-13537] - Wild-type (WT) and Brg1 knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunocytochemistry/ Immunofluorescence: Brg1 Antibody (GT2712) [NBP3-13537]

Immunocytochemistry/ Immunofluorescence: Brg1 Antibody (GT2712) [NBP3-13537]

Immunocytochemistry/Immunofluorescence: Brg1 Antibody (GT2712) [NBP3-13537] - Brg1 antibody [GT2712] detects Brg1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Brg1 protein stained by Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:100. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining.
Immunohistochemistry-Frozen: Brg1 Antibody (GT2712) [NBP3-13537]

Immunohistochemistry-Frozen: Brg1 Antibody (GT2712) [NBP3-13537]

Immunohistochemistry-Frozen: Brg1 Antibody (GT2712) [NBP3-13537] - Brg1 antibody [GT2712] detects Brg1 protein by immunohistochemical analysis. Sample: Frozen-sectioned rat E13.5 brain. Green: Brg1 stained by Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:250. Red: SOX2, stained by SOX2 antibody [N1C3] (NBP1-33766) diluted at 1:500. Blue: Fluoroshield with DAPI.
Western Blot: Brg1 Antibody (GT2712) [NBP3-13537]

Western Blot: Brg1 Antibody (GT2712) [NBP3-13537]

Western Blot: Brg1 Antibody (GT2712) [NBP3-13537] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:1000.
Western Blot: Brg1 Antibody (GT2712) [NBP3-13537]

Western Blot: Brg1 Antibody (GT2712) [NBP3-13537]

Western Blot: Brg1 Antibody (GT2712) [NBP3-13537] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:1000.
Western Blot: Brg1 Antibody (GT2712) [NBP3-13537]

Western Blot: Brg1 Antibody (GT2712) [NBP3-13537]

Western Blot: Brg1 Antibody (GT2712) [NBP3-13537] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:1000.
Immunohistochemistry-Paraffin: Brg1 Antibody (GT2712) [NBP3-13537]

Immunohistochemistry-Paraffin: Brg1 Antibody (GT2712) [NBP3-13537]

Immunohistochemistry-Paraffin: Brg1 Antibody (GT2712) [NBP3-13537] - Brg1 antibody [GT2712] detects Brg1 protein at nucleus in human oral carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Brg1 Antibody (GT2712) [NBP3-13537]

Immunohistochemistry-Paraffin: Brg1 Antibody (GT2712) [NBP3-13537]

Immunohistochemistry-Paraffin: Brg1 Antibody (GT2712) [NBP3-13537] - Brg1 antibody [GT2712] detects Brg1 protein at nucleus in human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for Brg1 Antibody (GT2712) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Brg1

BRG1 is encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Alternatively spliced transcripts have been found for this gene, but their full-length natures have not been determined.

Long Name

BRM/SWI2-related gene 1

Alternate Names

SMARCA4, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2

Gene Symbol

SMARCA4

Additional Brg1 Products

Product Documents for Brg1 Antibody (GT2712) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Brg1 Antibody (GT2712) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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