c-Myc Antibody (9E10) - BSA Free

Novus Biologicals | Catalog # NB600-302

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bovine, Drosophila

Cited:

Human, Mouse, Bovine, Insect - Drosophila

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunoblotting, ELISA, Sandwich ELISA, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Proximity Ligation Assay

Cited:

Western Blot, ELISA, Sandwich ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Proximity Ligation Assay, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 9E10

Format

BSA Free
View all c-Myc Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) of human c-Myc Antibody (9E10). [UniProt# P01106]

Epitope

Leucine zipper sequence (AEEQKLISEEDLL)

Specificity

Specific for the c-myc protein in random coil configuration, not as a helix. 9E10 does not react with V-myc.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

48.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for c-Myc Antibody (9E10) - BSA Free

Western Blot: c-Myc Antibody (9E10)BSA Free [NB600-302]

Western Blot: c-Myc Antibody (9E10)BSA Free [NB600-302]

c-Myc-Antibody-9E10-Western-Blot-NB600-302-img0022.jpg
Immunohistochemistry-Paraffin: c-Myc Antibody (9E10) - BSA Free [NB600-302]

Immunohistochemistry-Paraffin: c-Myc Antibody (9E10) - BSA Free [NB600-302]

Immunohistochemistry-Paraffin: c-Myc Antibody (9E10) [NB600-302] - c-Myc was detected in immersion fixed paraffin-embedded sections of human breast cancer using anti-human mouse monoclonal antibody (Catalog # NB600-302, clone 9E10) at 1:50 dilution overnight at 4 C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue).
Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
Simple Western: c-Myc Antibody (9E10)BSA Free [NB600-302]

Simple Western: c-Myc Antibody (9E10)BSA Free [NB600-302]

Simple Western: c-Myc Antibody (9E10) [NB600-302] - Simple Western lane view shows a specific band for c-Myc in 0.5 mg/ml of Jurkat lysate. This experiment as performed under reducing conditions using the 12-230 kDa separation system.
Western Blot: c-Myc Antibody (9E10)BSA Free [NB600-302]

Western Blot: c-Myc Antibody (9E10)BSA Free [NB600-302]

c-Myc-Antibody-9E10-BSA-Free-Western-Blot-NB600-302-img0026.jpg
Immunocytochemistry/ Immunofluorescence: c-Myc Antibody (9E10) - BSA Free [NB600-302]

Immunocytochemistry/ Immunofluorescence: c-Myc Antibody (9E10) - BSA Free [NB600-302]

Immunocytochemistry/Immunofluorescence: c-Myc Antibody (9E10) [NB600-302] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-c-Myc (9E10) at 10 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Flow (Intracellular): c-Myc Antibody (9E10) - BSA Free [NB600-302]

Flow (Intracellular): c-Myc Antibody (9E10) - BSA Free [NB600-302]

Flow (Intracellular): c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E10) NB600-302PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.. Using the PE format of this antibody.
Western Blot: c-Myc Antibody (9E10)BSA Free [NB600-302]

Western Blot: c-Myc Antibody (9E10)BSA Free [NB600-302]

Western Blot: c-Myc Antibody (9E10) [NB600-302] - Analysis of c-myc in Jurkat cell lysates using NB600-302.
Western Blot: c-Myc Antibody (9E10)BSA Free [NB600-302]

Western Blot: c-Myc Antibody (9E10)BSA Free [NB600-302]

c-Myc-Antibody-9E10-Western-Blot-NB600-302-img0025.jpg
Flow Cytometry: c-Myc Antibody (9E10) - BSA Free [NB600-302]

Flow Cytometry: c-Myc Antibody (9E10) - BSA Free [NB600-302]

Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E10) NB600-302AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow Cytometry: c-Myc Antibody (9E10) - BSA Free [NB600-302]

Flow Cytometry: c-Myc Antibody (9E10) - BSA Free [NB600-302]

Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on Jurkat cells with c-Myc Antibody (9E10) NB600-302R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.
Flow (Intracellular): c-Myc Antibody (9E10) - BSA Free [NB600-302]

Flow (Intracellular): c-Myc Antibody (9E10) - BSA Free [NB600-302]

Flow (Intracellular): c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E10) NB600-302F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.. Using the FITC format of this antibody.
c-Myc Antibody (9E10) - BSA Free

Western Blot: c-Myc Antibody (9E10) - BSA Free [NB600-302] -

Western Blot: c-Myc Antibody (9E10) - BSA Free [NB600-302] - NLRP2 interacts with SCMC components TLE6, OOEP, FILIA & NLRP5.(A) NLRP2 was overexpressed with myc-tagged OOEP, TLE6, NLRP5 & FILIA in HEK293T cells for 48 hours, immunoprecipitated with anti-NLRP2 or IgG as negative control & immunoblotted with anti-myc. Top panel shows specificity of anti-NLRP2 IP & bottom panel shows that NLRP2 binds to OOEP, TLE6, FILIA & NLRP5. Uncropped, full length western blots have been provided in Supplementary Figure 5. (B) Whole-mount immunofluorescence co-staining with anti-NLRP2 (green) & DAPI (blue) for nuclear staining on Nlrp2+/+ oocytes & embryos at 2-, 4-,16-cell & morula stages revealed a predominantly SCMC-like localization for NLRP2. Scale bars represent 200 μm. (C) Co-staining with anti-TLE6 (green) & DAPI (blue) of paraffin-embedded oocyte sections reveals a typical cortical stain of TLE6 in oocytes of Nlrp2+/+ dams but a more intense & diffuse stain in oocytes of Nlrp2tm1a/tm1a dams. Scale bars represent 10 μm. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/srep44667), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for c-Myc Antibody (9E10) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:50-1:200. Use reported in scientific literature (PMID 21315712)

Immunoblotting

reported in scientific literature (PMID 23750001)

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunohistochemistry-Frozen

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

1:10-1:500

Proximity Ligation Assay

reported in scientific literature (PMID 33298911)

Sandwich ELISA

reported in scientific literature (PMID 33122198)

Simple Western

1:200

Western Blot

0.5-2.0 ug/ml
Application Notes

See Simple Western Antibody Database for Simple Western validation: tested in Jurkat lysate (0.5 mg/ml); antibody dilution of 1:200; separated by size

Reviewed Applications

Read 4 reviews rated 4.3 using NB600-302 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: c-Myc

The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is a multifunctional, nuclear phosphoprotein that functions as a transcription factor with a theoretical molecular weight of 62kDa. However, c-Myc is extremely labile and is degraded very quickly even in extracts prepared with boiling SDS sample buffer, such that a molecular weight of ~40 kDa has been observed. c-Myc is part of a heterodimeric complex with Max that acts as a potent transcriptional activator. c-Myc is modified by glycosylation and phosphorylation and has been shown to interact with numerous proteins including SMAD2, SMAD3, LSD1/KDM1A, MAD, and Sp1 (1).

A basic Helix-Loop-Helix, Leucine Zipper domain (bHLH/LZ), designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with other bHLH-Zip proteins, Mad and Mxi1. c-Myc plays a role in cell cycle progression, apoptosis, cellular transformation and angiogenesis (2). Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of cancers including B-cell Lymphomas, acute myeloid leukemia, glioblastoma, stomach adenocarcinoma, and prostate adenocarcinoma (3).

References

1. Wilkinson, D. S., Tsai, W. W., Schumacher, M. A., & Barton, M. C. (2008). Chromatin-bound p53 anchors activated Smads and the mSin3A corepressor to confer transforming-growth-factor-beta-mediated transcription repression. Mol Cell Biol, 28(6), 1988-1998. doi:10.1128/mcb.01442-07

2. Pedrosa, A. R., Bodrug, N., Gomez-Escudero, J., Carter, E. P., Reynolds, L. E., Georgiou, P. N.,... Hodivala-Dilke, K. M. (2019). Tumor Angiogenesis Is Differentially Regulated by Phosphorylation of Endothelial Cell Focal Adhesion Kinase Tyrosines-397 and -861. Cancer Res, 79(17), 4371-4386. doi:10.1158/0008-5472.Can-18-3934

3. Nagasaka, M., Tsuzuki, K., Ozeki, Y., Tokugawa, M., Ohoka, N., Inoue, Y., & Hayashi, H. (2019). Lysine-Specific Demethylase 1 (LSD1/KDM1A) Is a Novel Target Gene of c-Myc. Biol Pharm Bull, 42(3), 481-488. doi:10.1248/bpb.b18-00892

Long Name

v-Myc Avian Myelocytomatosis Viral Oncogene Homolog (Avian)

Alternate Names

cMyc, Myc, Myc2, Niard, Nird, 9E10 anti-Myc, 9E10 Myc

Entrez Gene IDs

4609 (Human)

Gene Symbol

MYC

UniProt

P01106

Additional c-Myc Products

Product Documents for c-Myc Antibody (9E10) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for c-Myc Antibody (9E10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for c-Myc Antibody (9E10) - BSA Free

Customer Reviews for c-Myc Antibody (9E10) - BSA Free (4)

4.3 out of 5
4 Customer Ratings
5 Stars
50%
4 Stars
25%
3 Stars
25%
2 Stars
0%
1 Stars
0%

Have you used c-Myc Antibody (9E10) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card
Showing  1 - 4 of 4 reviews Showing All
Filter By:
Clear Filters X
  • c-Myc Antibody (9E10)
    Name: Phil Hill
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 05/31/2019
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Jurkat T-Cells
    Species: Human
    Verified Customer | Posted 11/30/2011
  • Name: Astrid Spannhoff
    Application: Western Blot
    Sample Tested: HeLa cell lysate, Sample Amount: 10 ul
    Species: Human
    Verified Customer | Posted 11/23/2010
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: 293T Cell Lysates overexpressing myc-tagged fusion proteins, Sample Amount: 50 ug
    Species: Human
    Verified Customer | Posted 08/10/2010

There are no reviews that match your criteria.

Showing  1 - 4 of 4 reviews Showing All

Protocols

View specific protocols for c-Myc Antibody (9E10) - BSA Free (NB600-302):


Western Blot Protocol
1. Perform SDS-PAGE (4-12% MOPS) on samples to be analyzed, loading 25 ug of total protein per lane.
2. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
3. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
4. Rinse the blot in TBS for approximately 5 minutes.
5. Block the membrane using 5% NFDM + 1% BSA in TBS + Tween, 1 hour at RT.
6. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each.
7. Dilute the mouse anti-c-myc primary antibody (NB600-302) in blocking buffer and incubate 1 hour at room temperature.
8. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each.
9. Apply the diluted mouset-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).
Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%, provided it does not interfere with antibody-antigen binding.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for c-Myc Antibody (9E10) - BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: I am interested in using clone 9E10 and would like to confirm if it recognizes p64 version of the protein as well as p67 version of the protein

    A: Yes, it recognizes both form of c-Myc protein (p64 and p67), based on literature and other scientific material.

  • Q: This antibody NB600-302 is raised in Mouse. It is suggesting that it will work in Mouse system. Please explain, since I am trying to implement this antibody in the mouse system.

    A: NB600-302 is a Mouse Monoclonal antibody, and it is fine for use on Mouse samples. Generally, there are no issues with mouse on mouse antibody if there are adequate blocking and washing steps.

  • Q: I am interested in using clone 9E10 and would like to confirm if it recognizes p64 version of the protein as well as p67 version of the protein

    A: Yes, it recognizes both form of c-Myc protein (p64 and p67), based on literature and other scientific material.

  • Q: This antibody NB600-302 is raised in Mouse. It is suggesting that it will work in Mouse system. Please explain, since I am trying to implement this antibody in the mouse system.

    A: NB600-302 is a Mouse Monoclonal antibody, and it is fine for use on Mouse samples. Generally, there are no issues with mouse on mouse antibody if there are adequate blocking and washing steps.

Showing  1 - 2 of 2 FAQs Showing All
View all FAQs for Antibodies