Canine CD25/IL-2 R alpha Antibody

(1 Review)
  
  • Species Reactivity
    Canine
  • Specificity
    Detects canine CD25/IL-2 R alpha in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human CD25/IL-2 R alpha, recombinant mouse CD25/IL-2 R alpha, and recombinant rat CD25/IL-2 R alpha is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant canine CD25/IL-2 R alpha
    Tyr19-Ile238
    Accession # BAI49682
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of CD25/IL-2 R alpha in Canine Blood Monocytes by Flow Cytometry. Canine peripheral blood monocytes were stained with Sheep Anti-Canine CD25/IL‑2 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6227) followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126) and Allophycocyanin-conjugated Anti-Canine CD4. Quadrant markers were set based on control antibody staining (Catalog # 5-001-A).

Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD25/IL-2 R alpha

IL-2 receptor alpha (IL-2 R alpha ), also known as CD25, is a 55 kDa type I membrane glycoprotein that belongs to the family of cytokine receptors that utilize the common gamma chain subunit ( gamma c). IL‑2 R alpha is primarily expressed on activated T cells and on regulatory T cells (Treg) (1‑3). The canine IL-2 R alpha cDNA encodes a 268 amino acid (aa) precursor that includes a 20 aa signal peptide, a 217 aa extracellular domain (ECD) with two Sushi domains, a 19 aa transmembrane segment, and an 12 aa cytoplasmic domain (4). Within the ECD, canine IL-2 R alpha shares 49% - 60% aa sequence identity with human, mouse, and rat IL-2 R alpha. IL-2 R beta (CD122) and gamma c (IL‑2 R gamma /CD132) dimerize to form a constitutively expressed intermediate affinity IL-2 receptor (5, 6). By itself, IL-2 R alpha binds IL-2 with low affinity. It associates with IL-2 R beta and gamma c to generate a ternary high affinity IL-2 receptor complex (7). A soluble form of IL‑2 R alpha can be generated by proteolytic cleavage of the cell surface receptor, rendering the T cell unresponsive to IL-2 (8, 9). Increased serum levels of soluble IL‑2 R alpha are found in some cancers and immune disorders (10). IL-2 R alpha is required for activation induced cell death (AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones (11, 12). IL-2 R alpha is also required for the development of CD4+CD25+ Treg which suppress autoreactive CD4+ T cells, thereby contributing to peripheral T cell homeostasis (11‑13).

  • References:
    1. Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
    2. Kovanen, P.E. and Leonard, W.J. (2004) Immunol. Rev. 202:67.
    3. Bluestone, J.A. and Tang, Q. (2005) Curr. Opin. Immunol. 17:638.
    4. Rissetto, K.C. et al. (2010) Vet. Immunol. Immunopathol. 135:137.
    5. Hatakeyama, M. et al. (1989) Science 244:551.
    6. Takeshita, T. et al. (1992) Science 257:379.
    7. Wang, X. et al. (2005) Science 310:1159.
    8. Wagner, D.K. et al. (1986) J. Immunol. 137:592.
    9. Schulz, O. et al. (1998) J. Exp. Med. 187:271.
    10. Witkowska, A.M. (2005) Mediat. Inflamm. 2005:121.
    11. Willerford, D.M. et al. (1995) Immunity 3:521.
    12. Van Parijs, L. et al. (1997) J. Immunol. 158:3738.
    13. Almeida, A.R.M. et al. (2002) J. Immunol. 169:4850.
  • Long Name:
    Interleukin 2 Receptor alpha
  • Entrez Gene IDs:
    3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine)
  • Alternate Names:
    CD25 antigen; CD25; IDDM10; IL-2 R alpha; IL-2 receptor subunit alpha; IL2R alpha; IL-2R subunit alpha; IL2R; IL2RA; IL-2Ra; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR
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Average Rating: 4 (Based on 1 review)

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Western Blot Canine CD25/IL-2 R alpha Antibody AF6227
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 WB Anonymous 06/28/2016
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Western Blot Canine CD25/IL-2 R alpha Antibody AF6227
Western Blot: Canine CD25/IL-2 R alpha Antibody [AF6227]

Summary

ApplicationWestern Blot
Sample TestedFetal Dog kidney,HEK293 human embryonic kidney cell line,canine melanoma cell

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