Key Product Details

Species Reactivity

Canine

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all CD25/IL-2R alpha Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant canine CD25/IL-2 R alpha
Tyr19-Ile238
Accession # BAI49682

Specificity

Detects canine CD25/IL-2 R alpha in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human CD25/IL-2 R alpha, recombinant mouse CD25/IL-2 R alpha, and recombinant rat CD25/IL-2 R alpha is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Canine CD25/IL-2R alpha Antibody

Detection of CD25/IL-2 Ra antibody in Canine Blood Monocytes antibody by Flow Cytometry.

Detection of CD25/IL-2 R alpha in Canine Blood Monocytes by Flow Cytometry.

Canine peripheral blood monocytes were stained with Sheep Anti-Canine CD25/IL-2 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6227) followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126) and Allophycocyanin-conjugated Anti-Canine CD4. Quadrant markers were set based on control antibody staining (Catalog # 5-001-A).

Applications for Canine CD25/IL-2R alpha Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Canine peripheral blood monocytes

Reviewed Applications

Read 1 review rated 4 using AF6227 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: CD25/IL-2R alpha

IL-2 receptor alpha (IL-2 R alpha ), also known as CD25, is a 55 kDa type I membrane glycoprotein that belongs to the family of cytokine receptors that utilize the common gamma chain subunit ( gamma c). IL‑2 R alpha is primarily expressed on activated T cells and on regulatory T cells (Treg) (1‑3). The canine IL-2 R alpha cDNA encodes a 268 amino acid (aa) precursor that includes a 20 aa signal peptide, a 217 aa extracellular domain (ECD) with two Sushi domains, a 19 aa transmembrane segment, and an 12 aa cytoplasmic domain (4). Within the ECD, canine IL-2 R alpha shares 49% - 60% aa sequence identity with human, mouse, and rat IL-2 R alpha. IL-2 R beta (CD122) and gamma c (IL‑2 R gamma /CD132) dimerize to form a constitutively expressed intermediate affinity IL-2 receptor (5, 6). By itself, IL-2 R alpha binds IL-2 with low affinity. It associates with IL-2 R beta and gamma c to generate a ternary high affinity IL-2 receptor complex (7). A soluble form of IL‑2 R alpha can be generated by proteolytic cleavage of the cell surface receptor, rendering the T cell unresponsive to IL-2 (8, 9). Increased serum levels of soluble IL‑2 R alpha are found in some cancers and immune disorders (10). IL-2 R alpha is required for activation induced cell death (AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones (11, 12). IL-2 R alpha is also required for the development of CD4+CD25+ Treg which suppress autoreactive CD4+ T cells, thereby contributing to peripheral T cell homeostasis (11‑13).

References

  1. Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
  2. Kovanen, P.E. and Leonard, W.J. (2004) Immunol. Rev. 202:67.
  3. Bluestone, J.A. and Tang, Q. (2005) Curr. Opin. Immunol. 17:638.
  4. Rissetto, K.C. et al. (2010) Vet. Immunol. Immunopathol. 135:137.
  5. Hatakeyama, M. et al. (1989) Science 244:551.
  6. Takeshita, T. et al. (1992) Science 257:379.
  7. Wang, X. et al. (2005) Science 310:1159.
  8. Wagner, D.K. et al. (1986) J. Immunol. 137:592.
  9. Schulz, O. et al. (1998) J. Exp. Med. 187:271.
  10. Witkowska, A.M. (2005) Mediat. Inflamm. 2005:121.
  11. Willerford, D.M. et al. (1995) Immunity 3:521.
  12. Van Parijs, L. et al. (1997) J. Immunol. 158:3738.
  13. Almeida, A.R.M. et al. (2002) J. Immunol. 169:4850.

Long Name

Interleukin 2 Receptor alpha

Alternate Names

CD25, IL-2 R alpha, IL-2Ra, IL2R alpha, IL2RA

Entrez Gene IDs

3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine); 102123605 (Cynomolgus Monkey)

Gene Symbol

IL2RA

UniProt

BAI49682

Additional CD25/IL-2R alpha Products

Product Documents for Canine CD25/IL-2R alpha Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Canine CD25/IL-2R alpha Antibody

For research use only

Customer Reviews for Canine CD25/IL-2R alpha Antibody (1)

4 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Canine CD25/IL-2R alpha Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Canine CD25/IL-2 R alpha Antibody
    Name: ABDUL MOHIN SAJIB
    Application: Western Blot
    Sample Tested: Fetal Dog kidney, HEK293 human embryonic kidney cell line and canine melanoma cell
    Species: Human and Canine
    Verified Customer | Posted 06/28/2016
    Canine CD25/IL-2R alpha Antibody AF6227

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies