Detection of CD44 in Canine Blood-Derived Lymphocytes by Flow Cytometry. Canine blood-derived lymphocytes were stained with Mouse Anti-Canine CD44 APC‑conjugated Monoclonal Antibody (Catalog # FAB5449A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Canine CD44 (also known as HCAM and Hermes) is a ubiquitously expressed 85‑90 kDa type I transmembrane glycoprotein that binds to hyaluronan, laminin, fibronectin, selectins, osteopontin, and chondroitin sulfate, and is involved in matrix adhesion, lymphocyte activation, and lymph node homing. The CD44 protein is expressed as a family of molecular isoforms that, in human and presumably in canine, are generated by alternative splicing and variable posttranslational modification. It is reported to form a complex with CD147. Within the N‑terminal invariant portion of the ECD (aa 14‑191), canine CD44 shares 90%, 83%, and 82% identity with human, mouse, and rat CD44, respectively.
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