CCR7 Antibody

Immunohistochemistry-Paraffin: CCR7 Antibody [NB100-712]

Immunohistochemistry-Paraffin: CCR7 Antibody [NB100-712] - Human spleen

Immunohistochemistry: CCR7 Antibody [NB100-712] -

Immunohistochemistry: CCR7 Antibody [NB100-712] - M1 expression pattern changed in trabecular bone. Immunohistochemical staining was performed in sagittal serial sections of medial condyles from 6-week-old rats within the epiphyseal region. The region with the solid line indicates areas of interest to be enlarged in the following image. Representative staining with the anticluster of differentiation 68 (CD68) antibody demonstrated that CD68+ cells were either attached (black arrows) or unattached (white arrows) to the bone surfaces with a variety of sizes & shapes (a, b). TRAP+ cells showed multinucleated morphology (black arrows) which is similar to the CD68+ cells (c, d). Inducible nitric oxide synthase-positive (iNOS+) & C-C chemokine receptor type 7-positive (CCR7+) cells (black arrow) were attached to the bone surfaces with an elongated morphology (e, f, i, j). Arginase1+ & cluster of differentiation 163-positive (CD163+) cells (white arrow) represented the predominant population in the reticular connective tissue with similar expression patterns as in the cortical bone (g, h, k, l). No positive staining was found in isotype controls (data not shown). Most of the M1-labeled cells were found attached to bone surfaces instead of M2-labeled cells (m). Representative images from three independent experiments are shown. FOV, field of view. Data shown as the mean±s.d. (*P<0.05). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29263936), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: CCR7 Antibody [NB100-712] -

Immune response of macrophages after blocking Piezo1. (A) IF staining of CCR7 and CD206 in the Control, LPS and GsMTx4+LPS groups after 4-day-cultured. M1 macrophages were marked with CCR7 (red), M2 macrophages with CD206 (green), and nuclei with DAPI (blue). (B) Semi-quantitative analysis of CCR7 and CD163 in each group. (C) Flow cytometry analysis of RAW264. 7 cells in the Control, LPS and GsMTx4+LPS groups. Q6 represents M1 types (F4/80+/iNOS+) and Q10 represents M2 types (F4/80+/CD206+). (D) Expressions of Piezo1 and inflammation-related genes (Tnfa and Il1b) in macrophages cultured for 4 days. (E) Western blotting analysis of PIEZO1, IL-1B and TNF-A in RAW264. 7 cultured for 4 days. (F) Intracellular ROS levels of RAW264. 7 cultured for 4 days. (G) Concentration of inflammatory cytokines in macrophage medium detected by ELISA. *P < 0. 05; **P < 0. 01; ***P < 0. 001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37261355), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: CCR7 Antibody [NB100-712] -

Immune response of macrophages after activating Piezo1. (A) IF staining of CCR7 and CD206 in the Control, LPS and Yoda1+LPS groups after 4-day-cultured. M1 macrophages were marked with CCR7 (red), M2 macrophages with CD206 (green), and nuclei with DAPI (blue). (B) Semi-quantitative analysis of CCR7 and CD206. (C) Flow cytometry analysis of RAW264. 7 cells in the Control, LPS and Yoda1+LPS groups. Q6 represents M1 types (F4/80+/iNOS+) and Q10 represents M2 types (F4/80+/CD206+). (D)Piezo1, Tnfa and Il1b genes expressions in macrophages cultured for 4 days. (E) Western blotting analysis of PIEZO1, TNF-A and IL-1B in RAW264. 7 cultured for 4 days. (F) Intracellular ROS levels of RAW264. 7 cultured for 4 days. (G) Concentration of inflammatory cytokines in macrophages medium detected by ELISA. *P < 0. 05; **P < 0. 01; ***P < 0. 001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37261355), licensed under a CC-BY license. Not internally tested by Novus Biologicals.