CCR7 Antibody

Novus Biologicals | Catalog # NB100-712

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, In vivo assay

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, In vivo assay, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Synthetic peptide: DPGKPRKNVLVVALLVIFQVC, corresponding to amino acids 2-22 of Mouse CCR7.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 25831137), (25803728).

Localization

Integral membrane protein.

Specificity

Peptide sequence is 50 % identical to other mouse chemokine receptors in this region.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Goat CCR7 Antibody (NB100-712) is a polyclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. Anti-CCR7 Antibody: Cited in 43 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CCR7 Antibody

Immunohistochemistry-Paraffin: CCR7 Antibody [NB100-712]

Immunohistochemistry-Paraffin: CCR7 Antibody [NB100-712]

CCR7-Antibody-Immunohistochemistry-Paraffin-NB100-712-img0006.jpg
Immunohistochemistry-Paraffin: CCR7 Antibody [NB100-712]

Immunohistochemistry-Paraffin: CCR7 Antibody [NB100-712]

Immunohistochemistry-Paraffin: CCR7 Antibody [NB100-712] - Mouse spleen
Immunohistochemistry-Paraffin: CCR7 Antibody [NB100-712]

Immunohistochemistry-Paraffin: CCR7 Antibody [NB100-712]

Immunohistochemistry-Paraffin: CCR7 Antibody [NB100-712] - Mouse spleen
Immunohistochemistry-Paraffin: CCR7 Antibody [NB100-712]

Immunohistochemistry-Paraffin: CCR7 Antibody [NB100-712]

Immunohistochemistry-Paraffin: CCR7 Antibody [NB100-712] - Human spleen
CCR7 Antibody

Immunohistochemistry: CCR7 Antibody [NB100-712] -

Immunohistochemistry: CCR7 Antibody [NB100-712] - M1 expression pattern changed in trabecular bone. Immunohistochemical staining was performed in sagittal serial sections of medial condyles from 6-week-old rats within the epiphyseal region. The region with the solid line indicates areas of interest to be enlarged in the following image. Representative staining with the anticluster of differentiation 68 (CD68) antibody demonstrated that CD68+ cells were either attached (black arrows) or unattached (white arrows) to the bone surfaces with a variety of sizes & shapes (a, b). TRAP+ cells showed multinucleated morphology (black arrows) which is similar to the CD68+ cells (c, d). Inducible nitric oxide synthase-positive (iNOS+) & C-C chemokine receptor type 7-positive (CCR7+) cells (black arrow) were attached to the bone surfaces with an elongated morphology (e, f, i, j). Arginase1+ & cluster of differentiation 163-positive (CD163+) cells (white arrow) represented the predominant population in the reticular connective tissue with similar expression patterns as in the cortical bone (g, h, k, l). No positive staining was found in isotype controls (data not shown). Most of the M1-labeled cells were found attached to bone surfaces instead of M2-labeled cells (m). Representative images from three independent experiments are shown. FOV, field of view. Data shown as the mean±s.d. (*P<0.05). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29263936), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for CCR7 Antibody

Application
Recommended Usage

ELISA

1:100000

Flow Cytometry

1:10

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:250

Immunohistochemistry-Frozen

1:250

Immunohistochemistry-Paraffin

1:250

Western Blot

1:1000
Application Notes
Use in ICC/IF reported in scintific literature (PMID: 29311580). Use in IHC-Frozen reported in scientific literature (PMID: 25803728). Use in In Vivo assays reported in scientific literature (PMID: 30541701).

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

10 mM KHPO4, 0.14 M NaCl, 1.0 mg/mL BSA

Preservative

0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CCR7

Chemokine receptor 7 (CCR7) is a G-protein coupled receptor (GPCR) that binds chemokine ligand 19 (CCL19) and chemokine ligand 21 (CCL21) and, together, this receptor-ligand interaction functions in inflammatory and adaptive immune response (1,2). The CCR7 protein contains 7-transmembrane spanning alpha helices and is 378 amino acids (aa) in length, with a theoretical molecular weight of 42.8 kDa (3,4). CCR7 is expressed on several cells within the immune system, including naive T cells, central memory T cells, regulatory T cells, naive B cells, a subset of double negative and single positive thymocytes, and mature dendritic cells (DCs) (1, 3).

The primary role of the CCR7/CCL19/CCL21 chemokine signaling axis is homing T cells and DCs to lymph nodes and lymphoid tissues to initiate an immune response (1,2,5,6). In the context of cancer, the CCR7 signaling axis appears to have two opposing roles (2). Downregulation of CCR7 on CD8+ T cells contributes to effector cell migration and anti-cancer activities via cytotoxic tumor-infiltrating lymphocytes (2). However, upregulation of CCR7 by cancer cells can result in cancer cell migration and metastasis (2). Overexpression of CCR7 has been implicated in a variety of cancers including breast, cervical, gastric, head and neck cell carcinoma, and prostate (1,2,7). Studies in breast cancer have found that hypoxia increases CCR7 expression, and this activation can affect cancer cell invasion, extravasation, proliferation, angiogenesis, and metastasis through induction of multiple signaling transduction pathways such as PI3K/AKT, MAPK, and JAK/STAT (5,7).

Given its important role in inflammation and immune response, several strategies have been employed to target the CCR7 signaling axis for cancer immunotherapy (2). Some cancer immunotherapies under investigation include intra-tumoral administration of CCL19 and CCL21, introduction of patient-derived cells transfected to express CCR7 or its ligands, and vaccines (2). Further interrogation of CCR7/CCL19/CCL21 signaling axis is required to develop better therapeutic strategies for cancer treatment.

References:

1. Comerford, I., Harata-Lee, Y., Bunting, M. D., Gregor, C., Kara, E. E., & McColl, S. R. (2013). A myriad of functions and complex regulation of the CCR7/CCL19/CCL21 chemokine axis in the adaptive immune system. Cytokine & growth factor reviews, 24(3), 269-283. https://doi.org/10.1016/j.cytogfr.2013.03.001

2. Salem, A., Alotaibi, M., Mroueh, R., Basheer, H. A., & Afarinkia, K. (2021). CCR7 as a therapeutic target in Cancer. Biochimica et biophysica acta. Reviews on cancer, 1875(1), 188499. https://doi.org/10.1016/j.bbcan.2020.188499

3. Yan, Y., Chen, R., Wang, X., Hu, K., Huang, L., Lu, M., & Hu, Q. (2019). CCL19 and CCR7 Expression, Signaling Pathways, and Adjuvant Functions in Viral Infection and Prevention. Frontiers in cell and developmental biology, 7, 212. https://doi.org/10.3389/fcell.2019.00212

4. Uniprot (P32248)

5. Korbecki, J., Grochans, S., Gutowska, I., Barczak, K., & Baranowska-Bosiacka, I. (2020). CC Chemokines in a Tumor: A Review of Pro-Cancer and Anti-Cancer Properties of Receptors CCR5, CCR6, CCR7, CCR8, CCR9, and CCR10 Ligands. International journal of molecular sciences, 21(20), 7619. https://doi.org/10.3390/ijms21207619

6. Sanchez-Sanchez, N., Riol-Blanco, L., & Rodriguez-Fernandez, J. L. (2006). The multiple personalities of the chemokine receptor CCR7 in dendritic cells. Journal of immunology (Baltimore, Md. : 1950), 176(9), 5153-5159. https://doi.org/10.4049/jimmunol.176.9.5153

7. Rizeq, B., & Malki, M. I. (2020). The Role of CCL21/CCR7 Chemokine Axis in Breast Cancer Progression. Cancers, 12(4), 1036. https://doi.org/10.3390/cancers12041036

Alternate Names

BLR2, CC-CKR-7, CCR7, CD197, CDw197, CMKBR7, EBI1

Entrez Gene IDs

1236 (Human); 12775 (Mouse)

Gene Symbol

CCR7

UniProt

Additional CCR7 Products

Product Documents for CCR7 Antibody

Certificate of Analysis

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Product Specific Notices for CCR7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CCR7 Antibody

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    • Q: Does this product require specific antigen retrieval? I wonder if you tested the different antigen retrieval buffers and you can tell which one I should use?

      A: We recommend antigen retrieval for all of our products. We recommend citrate buffer at a pH of 6.0. Here is a link to our antigen retrieval protocol.
    • A: NB100-712 is a goat polyclonal and it will be a mixture of isotypes.
    • A: We unfortunately do not offer our NB100-712 without sodium azide. We do offer antibody clarification kits that will remove sodium azide.
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