CD117/c-kit Antibody (CL1657) - BSA Free

Name: CD117/c-kit Antibody (CL1657) - BSA Free
Brand: Novus Biologicals
SKU: NBP2-52975
Price: 269 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-52975

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # CL1657

Format

BSA Free

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Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P10721, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for CD117/c-kit Antibody (CL1657) - BSA Free

Western Blot: CD117/c-kit Antibody (CL1657) [NBP2-52975]

Western Blot: CD117/c-kit Antibody (CL1657) [NBP2-52975] - Lane 1: Marker [kDa], Lane 2: Human cell line RT-4

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (CL1657) [NBP2-52975]

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (CL1657) [NBP2-52975] - Staining of human stomach shows strong positivity in a subset of lymphoid cells.

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (CL1657) [NBP2-52975]

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (CL1657) [NBP2-52975] - Staining of human cerebellum shows moderate positivity in neuropil of the molecular layer.

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (CL1657) [NBP2-52975]

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (CL1657) [NBP2-52975] - Staining of human colon shows strong immunoreactivity in a subset of lymphoid cells.

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (CL1657) [NBP2-52975]

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (CL1657) [NBP2-52975] - Staining of human pancreas shows absence of immunoreactivity (negative control).

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (CL1657) [NBP2-52975]

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (CL1657) [NBP2-52975] - Staining of human small intestine shows strong immunoreactivity in a subset of lymphoid cells (macrophages).

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Applications for CD117/c-kit Antibody (CL1657) - BSA Free

Application

Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD117/c-kit

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with it's ligand is critical for the development of hematopoietic stem cells. The 2B8 antibody does not block c-Kit activity.

Long Name

Stem Cell Factor Receptor

Alternate Names

c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR

Gene Symbol

KIT

Additional CD117/c-kit Products

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Product Specific Notices for CD117/c-kit Antibody (CL1657) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.