CD11b Antibody (CL1719) - BSA Free
Novus Biologicals | Catalog # NBP2-34490
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Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CD11b Antibody (CL1719) - BSA Free
Immunohistochemistry-Paraffin: CD11b Antibody (CL1719) [NBP2-34490]
Immunohistochemistry-Paraffin: CD11b Antibody (CL1719) [NBP2-34490] - Immunohistochemical staining in paraffin-embedded sections of human tonsil and skeletal muscle tissues using [NBP2-34490]. Corresponding ITGAM RNA-seq data are presented for the same tissues.Western Blot: CD11b Antibody (CL1719) [NBP2-34490]
Western Blot: CD11b Antibody (CL1719) [NBP2-34490] - Western Blot analysis using CD11b Antibody (CL1719) [NBP2-34490]. Lane 1: Marker [kDa] Lane 2: Human tonsil. Detection at ~190 kDa.Immunohistochemistry-Paraffin: CD11b Antibody (CL1719) [NBP2-34490]
Immunohistochemistry-Paraffin: CD11b Antibody (CL1719) [NBP2-34490] - Immunohistochemical staining in paraffin-embedded sections of human cerebral cortex using CD11b Antibody (CL1719) [NBP2-34490] shows moderate membranous positivity in microglia.Immunohistochemistry-Paraffin: CD11b Antibody (CL1719) [NBP2-34490]
Immunohistochemistry-Paraffin: CD11b Antibody (CL1719) [NBP2-34490] - Immunohistochemical staining in paraffin-embedded sections of human tonsil using CD11b Antibody (CL1719) [NBP2-34490] shows strong membranous positivity in germinal center cells.Immunohistochemistry-Paraffin: CD11b Antibody (CL1719) [NBP2-34490]
Immunohistochemistry-Paraffin: CD11b Antibody (CL1719) [NBP2-34490] - Immunohistochemical staining of human colon using CD11b Antibody (CL1719) [NBP2-34490] shows moderate membranous positivity in lymphoid cells.Immunohistochemistry-Paraffin: CD11b Antibody (CL1719) [NBP2-34490]
Immunohistochemistry-Paraffin: CD11b Antibody (CL1719) [NBP2-34490] - Immunohistochemical staining of Paraffin-embedded sections of human skeletal muscle using CD11b Antibody (CL1719) [NBP2-34490] shows no positivity in striated muscle fibers as expected.Applications for CD11b Antibody (CL1719) - BSA Free
Immunohistochemistry
Immunohistochemistry-Paraffin
Western Blot
Formulation, Preparation, and Storage
Purification
Formulation
Format
Preservative
Concentration
Shipping
Stability & Storage
Background: CD11b/Integrin alpha M
References
1. Rosetti, F., & Mayadas, T. N. (2016). The many faces of Mac-1 in autoimmune disease. Immunol Rev, 269(1), 175-193. doi:10.1111/imr.12373
2. Kim, D., Kim, T. H., Wu, G., Park, B. K., Ha, J. H., Kim, Y. S.,... Kwon, H. J. (2016). Extracellular Release of CD11b by TLR9 Stimulation in Macrophages. PLoS One, 11(3), e0150677. doi:10.1371/journal.pone.0150677
3. Christensen, J. E., Andreasen, S. O., Christensen, J. P., & Thomsen, A. R. (2001). CD11b expression as a marker to distinguish between recently activated effector CD8(+) T cells and memory cells. Int Immunol, 13(4), 593-600. doi:10.1093/intimm/13.4.593
4. Nath, S. K., Han, S., Kim-Howard, X., Kelly, J. A., Viswanathan, P., Gilkeson, G. S.,... Harley, J. B. (2008). A nonsynonymous functional variant in integrin-alpha(M) (encoded by ITGAM) is associated with systemic lupus erythematosus. Nat Genet, 40(2), 152-154. doi:10.1038/ng.71
Additional CD11b/Integrin alpha M Products
Product Documents for CD11b Antibody (CL1719) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CD11b Antibody (CL1719) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for CD11b Antibody (CL1719) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for CD11b Antibody (CL1719) - BSA Free
-
Q: Do you know if this CD11b antibody can be used in CD11b positive cell depletion?
A: This particular CD11b antibody is not reccomended for depletion studies due to the preservative used; however, our CD11b antibody NBP1-06650 is offered in a preservative free format and has been validated for blocking.
-
Q: I am looking for a Mac-1 (microglia cell marker) antibody to do immunostaining with my mouse brain and I checked, you have a lot of antibodies for this biomarker. I don't know which one is the best to be used for immunohistology on mouse brain tissue. Could you please help with this?
A:
I would recommend NB110-89474. This antibody has been published in 11 scientific articles, including several times in IHC in mouse. As long as CD11b is expressed in your sample, it should work just fine and is backed by our 100% Novus Guarantee.
-
Q: I'm not sure if this will work for me, can I get a free sample of your CD11b antibody to test?
A: We don't offer free samples but we do offer this CD11b antibody in a trial size of 0.025 ml at a reduced cost, all antibodies are backed by our 100% guarantee to work in all validated species and applications on the product's web page.
-
Q: Is CD11b antibody also staining neutrophils in the brain
A: CD11b is typically used as a marker for granulocytes and macrophages. You may need a combination of markers to specifically identify neutrophils
-
Q: Looking for a bone marrow macrophage marker can you recommend a CD11b antibody for ICC?
A: I would recommend NB110-89474 which is our most popular CD11b antibody and has been validated in ICC and cited in 18 publications.
-
Q: The protocol says to do enzymatic antigen retrieval before incubating with the CD11b antibody, which enzyme is used?
A: The protocol is referencting the PIER method using Proteinase K as the enzyme.
-
Q: We need a CD11b antibody for IHC-P on mouse tissue conjugated to FITC, do you have one that will work?
A: We have 3 CD11b antibodies for IHC-P and just one CD11 b antibody we offer conjugated to FITC, NB110-89474F.
-
Q: What types of cells can this CD11b antibody be used as a marker for?
A: A study used this CD11b antibody to stain granulocytes in mouse uterine tissue in IHC. CD11b is also expressed on other leukocytes such as monocytes, macrophages, and natural killer cells.
-
Q: Do you know if this CD11b antibody can be used in CD11b positive cell depletion?
A: This particular CD11b antibody is not reccomended for depletion studies due to the preservative used; however, our CD11b antibody NBP1-06650 is offered in a preservative free format and has been validated for blocking.
-
Q: I am looking for a Mac-1 (microglia cell marker) antibody to do immunostaining with my mouse brain and I checked, you have a lot of antibodies for this biomarker. I don't know which one is the best to be used for immunohistology on mouse brain tissue. Could you please help with this?
A:
I would recommend NB110-89474. This antibody has been published in 11 scientific articles, including several times in IHC in mouse. As long as CD11b is expressed in your sample, it should work just fine and is backed by our 100% Novus Guarantee.
-
Q: I'm not sure if this will work for me, can I get a free sample of your CD11b antibody to test?
A: We don't offer free samples but we do offer this CD11b antibody in a trial size of 0.025 ml at a reduced cost, all antibodies are backed by our 100% guarantee to work in all validated species and applications on the product's web page.
-
Q: Is CD11b antibody also staining neutrophils in the brain
A: CD11b is typically used as a marker for granulocytes and macrophages. You may need a combination of markers to specifically identify neutrophils
-
Q: Looking for a bone marrow macrophage marker can you recommend a CD11b antibody for ICC?
A: I would recommend NB110-89474 which is our most popular CD11b antibody and has been validated in ICC and cited in 18 publications.
-
Q: The protocol says to do enzymatic antigen retrieval before incubating with the CD11b antibody, which enzyme is used?
A: The protocol is referencting the PIER method using Proteinase K as the enzyme.
-
Q: We need a CD11b antibody for IHC-P on mouse tissue conjugated to FITC, do you have one that will work?
A: We have 3 CD11b antibodies for IHC-P and just one CD11 b antibody we offer conjugated to FITC, NB110-89474F.
-
Q: What types of cells can this CD11b antibody be used as a marker for?
A: A study used this CD11b antibody to stain granulocytes in mouse uterine tissue in IHC. CD11b is also expressed on other leukocytes such as monocytes, macrophages, and natural killer cells.
-
Q: Do you know if this CD11b antibody can be used in CD11b positive cell depletion?
A: This particular CD11b antibody is not reccomended for depletion studies due to the preservative used; however, our CD11b antibody NBP1-06650 is offered in a preservative free format and has been validated for blocking.
-
Q: I am looking for a Mac-1 (microglia cell marker) antibody to do immunostaining with my mouse brain and I checked, you have a lot of antibodies for this biomarker. I don't know which one is the best to be used for immunohistology on mouse brain tissue. Could you please help with this?
A:
I would recommend NB110-89474. This antibody has been published in 11 scientific articles, including several times in IHC in mouse. As long as CD11b is expressed in your sample, it should work just fine and is backed by our 100% Novus Guarantee.
-
Q: I'm not sure if this will work for me, can I get a free sample of your CD11b antibody to test?
A: We don't offer free samples but we do offer this CD11b antibody in a trial size of 0.025 ml at a reduced cost, all antibodies are backed by our 100% guarantee to work in all validated species and applications on the product's web page.
-
Q: Is CD11b antibody also staining neutrophils in the brain
A: CD11b is typically used as a marker for granulocytes and macrophages. You may need a combination of markers to specifically identify neutrophils
-
Q: Looking for a bone marrow macrophage marker can you recommend a CD11b antibody for ICC?
A: I would recommend NB110-89474 which is our most popular CD11b antibody and has been validated in ICC and cited in 18 publications.
-
Q: The protocol says to do enzymatic antigen retrieval before incubating with the CD11b antibody, which enzyme is used?
A: The protocol is referencting the PIER method using Proteinase K as the enzyme.
-
Q: We need a CD11b antibody for IHC-P on mouse tissue conjugated to FITC, do you have one that will work?
A: We have 3 CD11b antibodies for IHC-P and just one CD11 b antibody we offer conjugated to FITC, NB110-89474F.
-
Q: What types of cells can this CD11b antibody be used as a marker for?
A: A study used this CD11b antibody to stain granulocytes in mouse uterine tissue in IHC. CD11b is also expressed on other leukocytes such as monocytes, macrophages, and natural killer cells.
-
Q: Do you know if this CD11b antibody can be used in CD11b positive cell depletion?
A: This particular CD11b antibody is not reccomended for depletion studies due to the preservative used; however, our CD11b antibody NBP1-06650 is offered in a preservative free format and has been validated for blocking.
-
Q: I am looking for a Mac-1 (microglia cell marker) antibody to do immunostaining with my mouse brain and I checked, you have a lot of antibodies for this biomarker. I don't know which one is the best to be used for immunohistology on mouse brain tissue. Could you please help with this?
A:
I would recommend NB110-89474. This antibody has been published in 11 scientific articles, including several times in IHC in mouse. As long as CD11b is expressed in your sample, it should work just fine and is backed by our 100% Novus Guarantee.
-
Q: I'm not sure if this will work for me, can I get a free sample of your CD11b antibody to test?
A: We don't offer free samples but we do offer this CD11b antibody in a trial size of 0.025 ml at a reduced cost, all antibodies are backed by our 100% guarantee to work in all validated species and applications on the product's web page.
-
Q: Is CD11b antibody also staining neutrophils in the brain
A: CD11b is typically used as a marker for granulocytes and macrophages. You may need a combination of markers to specifically identify neutrophils
-
Q: Looking for a bone marrow macrophage marker can you recommend a CD11b antibody for ICC?
A: I would recommend NB110-89474 which is our most popular CD11b antibody and has been validated in ICC and cited in 18 publications.
-
Q: The protocol says to do enzymatic antigen retrieval before incubating with the CD11b antibody, which enzyme is used?
A: The protocol is referencting the PIER method using Proteinase K as the enzyme.
-
Q: We need a CD11b antibody for IHC-P on mouse tissue conjugated to FITC, do you have one that will work?
A: We have 3 CD11b antibodies for IHC-P and just one CD11 b antibody we offer conjugated to FITC, NB110-89474F.
-
Q: What types of cells can this CD11b antibody be used as a marker for?
A: A study used this CD11b antibody to stain granulocytes in mouse uterine tissue in IHC. CD11b is also expressed on other leukocytes such as monocytes, macrophages, and natural killer cells.
-
Q: Do you know if this CD11b antibody can be used in CD11b positive cell depletion?
A: This particular CD11b antibody is not reccomended for depletion studies due to the preservative used; however, our CD11b antibody NBP1-06650 is offered in a preservative free format and has been validated for blocking.
-
Q: I am looking for a Mac-1 (microglia cell marker) antibody to do immunostaining with my mouse brain and I checked, you have a lot of antibodies for this biomarker. I don't know which one is the best to be used for immunohistology on mouse brain tissue. Could you please help with this?
A:
I would recommend NB110-89474. This antibody has been published in 11 scientific articles, including several times in IHC in mouse. As long as CD11b is expressed in your sample, it should work just fine and is backed by our 100% Novus Guarantee.
-
Q: I'm not sure if this will work for me, can I get a free sample of your CD11b antibody to test?
A: We don't offer free samples but we do offer this CD11b antibody in a trial size of 0.025 ml at a reduced cost, all antibodies are backed by our 100% guarantee to work in all validated species and applications on the product's web page.
-
Q: Is CD11b antibody also staining neutrophils in the brain
A: CD11b is typically used as a marker for granulocytes and macrophages. You may need a combination of markers to specifically identify neutrophils
-
Q: Looking for a bone marrow macrophage marker can you recommend a CD11b antibody for ICC?
A: I would recommend NB110-89474 which is our most popular CD11b antibody and has been validated in ICC and cited in 18 publications.
-
Q: The protocol says to do enzymatic antigen retrieval before incubating with the CD11b antibody, which enzyme is used?
A: The protocol is referencting the PIER method using Proteinase K as the enzyme.
-
Q: We need a CD11b antibody for IHC-P on mouse tissue conjugated to FITC, do you have one that will work?
A: We have 3 CD11b antibodies for IHC-P and just one CD11 b antibody we offer conjugated to FITC, NB110-89474F.
-
Q: What types of cells can this CD11b antibody be used as a marker for?
A: A study used this CD11b antibody to stain granulocytes in mouse uterine tissue in IHC. CD11b is also expressed on other leukocytes such as monocytes, macrophages, and natural killer cells.
-
Q: Do you know if this CD11b antibody can be used in CD11b positive cell depletion?
A: This particular CD11b antibody is not reccomended for depletion studies due to the preservative used; however, our CD11b antibody NBP1-06650 is offered in a preservative free format and has been validated for blocking.
-
Q: I am looking for a Mac-1 (microglia cell marker) antibody to do immunostaining with my mouse brain and I checked, you have a lot of antibodies for this biomarker. I don't know which one is the best to be used for immunohistology on mouse brain tissue. Could you please help with this?
A:
I would recommend NB110-89474. This antibody has been published in 11 scientific articles, including several times in IHC in mouse. As long as CD11b is expressed in your sample, it should work just fine and is backed by our 100% Novus Guarantee.
-
Q: I'm not sure if this will work for me, can I get a free sample of your CD11b antibody to test?
A: We don't offer free samples but we do offer this CD11b antibody in a trial size of 0.025 ml at a reduced cost, all antibodies are backed by our 100% guarantee to work in all validated species and applications on the product's web page.
-
Q: Is CD11b antibody also staining neutrophils in the brain
A: CD11b is typically used as a marker for granulocytes and macrophages. You may need a combination of markers to specifically identify neutrophils
-
Q: Looking for a bone marrow macrophage marker can you recommend a CD11b antibody for ICC?
A: I would recommend NB110-89474 which is our most popular CD11b antibody and has been validated in ICC and cited in 18 publications.
-
Q: The protocol says to do enzymatic antigen retrieval before incubating with the CD11b antibody, which enzyme is used?
A: The protocol is referencting the PIER method using Proteinase K as the enzyme.
-
Q: We need a CD11b antibody for IHC-P on mouse tissue conjugated to FITC, do you have one that will work?
A: We have 3 CD11b antibodies for IHC-P and just one CD11 b antibody we offer conjugated to FITC, NB110-89474F.
-
Q: What types of cells can this CD11b antibody be used as a marker for?
A: A study used this CD11b antibody to stain granulocytes in mouse uterine tissue in IHC. CD11b is also expressed on other leukocytes such as monocytes, macrophages, and natural killer cells.
-
Q: Do you know if this CD11b antibody can be used in CD11b positive cell depletion?
A: This particular CD11b antibody is not reccomended for depletion studies due to the preservative used; however, our CD11b antibody NBP1-06650 is offered in a preservative free format and has been validated for blocking.
-
Q: I am looking for a Mac-1 (microglia cell marker) antibody to do immunostaining with my mouse brain and I checked, you have a lot of antibodies for this biomarker. I don't know which one is the best to be used for immunohistology on mouse brain tissue. Could you please help with this?
A:
I would recommend NB110-89474. This antibody has been published in 11 scientific articles, including several times in IHC in mouse. As long as CD11b is expressed in your sample, it should work just fine and is backed by our 100% Novus Guarantee.
-
Q: I'm not sure if this will work for me, can I get a free sample of your CD11b antibody to test?
A: We don't offer free samples but we do offer this CD11b antibody in a trial size of 0.025 ml at a reduced cost, all antibodies are backed by our 100% guarantee to work in all validated species and applications on the product's web page.
-
Q: Is CD11b antibody also staining neutrophils in the brain
A: CD11b is typically used as a marker for granulocytes and macrophages. You may need a combination of markers to specifically identify neutrophils
-
Q: Looking for a bone marrow macrophage marker can you recommend a CD11b antibody for ICC?
A: I would recommend NB110-89474 which is our most popular CD11b antibody and has been validated in ICC and cited in 18 publications.
-
Q: The protocol says to do enzymatic antigen retrieval before incubating with the CD11b antibody, which enzyme is used?
A: The protocol is referencting the PIER method using Proteinase K as the enzyme.
-
Q: We need a CD11b antibody for IHC-P on mouse tissue conjugated to FITC, do you have one that will work?
A: We have 3 CD11b antibodies for IHC-P and just one CD11 b antibody we offer conjugated to FITC, NB110-89474F.
-
Q: What types of cells can this CD11b antibody be used as a marker for?
A: A study used this CD11b antibody to stain granulocytes in mouse uterine tissue in IHC. CD11b is also expressed on other leukocytes such as monocytes, macrophages, and natural killer cells.
-
Q: Do you know if this CD11b antibody can be used in CD11b positive cell depletion?
A: This particular CD11b antibody is not reccomended for depletion studies due to the preservative used; however, our CD11b antibody NBP1-06650 is offered in a preservative free format and has been validated for blocking.
-
Q: I am looking for a Mac-1 (microglia cell marker) antibody to do immunostaining with my mouse brain and I checked, you have a lot of antibodies for this biomarker. I don't know which one is the best to be used for immunohistology on mouse brain tissue. Could you please help with this?
A:
I would recommend NB110-89474. This antibody has been published in 11 scientific articles, including several times in IHC in mouse. As long as CD11b is expressed in your sample, it should work just fine and is backed by our 100% Novus Guarantee.
-
Q: I'm not sure if this will work for me, can I get a free sample of your CD11b antibody to test?
A: We don't offer free samples but we do offer this CD11b antibody in a trial size of 0.025 ml at a reduced cost, all antibodies are backed by our 100% guarantee to work in all validated species and applications on the product's web page.
-
Q: Is CD11b antibody also staining neutrophils in the brain
A: CD11b is typically used as a marker for granulocytes and macrophages. You may need a combination of markers to specifically identify neutrophils
-
Q: Looking for a bone marrow macrophage marker can you recommend a CD11b antibody for ICC?
A: I would recommend NB110-89474 which is our most popular CD11b antibody and has been validated in ICC and cited in 18 publications.
-
Q: The protocol says to do enzymatic antigen retrieval before incubating with the CD11b antibody, which enzyme is used?
A: The protocol is referencting the PIER method using Proteinase K as the enzyme.
-
Q: We need a CD11b antibody for IHC-P on mouse tissue conjugated to FITC, do you have one that will work?
A: We have 3 CD11b antibodies for IHC-P and just one CD11 b antibody we offer conjugated to FITC, NB110-89474F.
-
Q: What types of cells can this CD11b antibody be used as a marker for?
A: A study used this CD11b antibody to stain granulocytes in mouse uterine tissue in IHC. CD11b is also expressed on other leukocytes such as monocytes, macrophages, and natural killer cells.