CD11b Antibody (M1/70.15) - BSA Free

Novus Biologicals | Catalog # NB600-1327

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rabbit

Cited:

Human, Mouse, Rat, Rabbit

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2b Lambda Clone # M1/70.15

Format

BSA Free
View all CD11b/Integrin alpha M Primary Antibodies »

Product Specifications

Immunogen

Rat Monoclonal CD11b Antibody (M1/70.15) was made to T cell enriched splenocytes from B10 mice.

Reactivity Notes

Mouse reactivity reported in a verified customer review. Rabbit reactivity reported in scientific literature (PMID: 28700779). Human reactivity reported in scientific literature (PMID: 28683563).

Marker

Microglia Marker, Myeloid Marker

Clonality

Monoclonal

Host

Rat

Isotype

IgG2b Lambda

Theoretical MW

127.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD11b Antibody (M1/70.15) - BSA Free

Immunohistochemistry: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Immunohistochemistry: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Immunohistochemistry: CD11b Antibody (M1/70.15) [NB600-1327] - Immunohistochemical analysis of frozen lymph node section using CD11b Antibody (M1/70.15) [NB600-1327] followed by staining with HRP-conjugated secondary antibody.
Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) [NB600-1327] - Characterization of microglia cultures by multiple staining with CD11b Antibody (M1/70.15) [NB600-1327] (panels a. & c.) and anti GFAP (panels b & d). Nuclei appear blue due to 4',6-diamidino-2-phenylindole (DAPI) counter staining.
Flow Cytometry: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Flow Cytometry: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Flow Cytometry: CD11b Antibody (M1/70.15) [NB600-1327] - A surface stain was performed on Raw264.7 cells with CD11b (M1/70.15) Antibody [NB600-1327R] (blue) and a matched isotype control (orange), both conjugated to DyLight 550. Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature.
Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody [M1/70.15] (NB600-1327) at 1ug/ml overnight at 4C and detected with an anti-rat DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody [M1/70.15] conjugated to Alexa Fluor 488 (NB600-1327AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) [NB600-1327] - Immunocytochemistry/Immunofluorescence (ICC/IF) analysis of RAW264.7 cells using CD11b antibody (M1/70.15) [NB600-1327] with Dylight 488 conjugated secondary (green). Nuclei and tubulin were stained with 4',6-diamidino-2-phenylindole (DAPI) (blue) and Dylight 550 (red).
Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) [NB600-1327] - Immunocytochemical/Immunofluorescent staining in untreated and treated mouse microglia cells using CD11b Antibody (M1/70.15) [NB600-1327]. Image from a verified customer review.
Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) [NB600-1327] - Immunocytochemical/Immunofluorescent analysis in mouse primary microglia. Cells were treated with lysophosphatidic acid (LPA) or LPA and an LPAR5 inhibitor in order to investigate the expression of iNOS after 24 hours. CD11b Antibody (M1/70.15) [NB600-1327] was used in a dilution 1:100 in order to stain primary murine microglia. Cy2 (green) and Cy3 (red) were used as secondary antibodies.
Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody [M1/70.15] conjugated to Alexa Fluor 647 (NB600-1327AF647) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Flow Cytometry: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Flow Cytometry: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Flow Cytometry: CD11b Antibody (M1/70.15) [NB600-1327] - Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b Antibody (M1/70.15) [NB600-1327].
Flow Cytometry: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Flow Cytometry: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Flow Cytometry: CD11b Antibody (M1/70.15) [NB600-1327] - Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b Antibody (M1/70.15) [NB600-1327]: RPE - Alexa Fluor 750
Flow Cytometry: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Flow Cytometry: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Flow Cytometry: CD11b Antibody (M1/70.15) [NB600-1327] - Staining of mouse peritoneal macrophages using the Alexa Fluor (R) 488 conjugate of CD11b Antibody (M1/70.15) [NB600-1327AF488].
CD11b Antibody (M1/70.15) - BSA Free

CD11b (M1/70.15) in Raw 264.7 Mouse Cell Line.

CD11b (M1/70.15) was detected in immersion fixed Raw 264.7 mouse macrophage cell line using Rat anti-CD11b (M1/70.15) Protein-G purified Monoclonal Antibody conjugated to DyLight 550 (Catalog # NB600-1327R) (red) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Applications for CD11b Antibody (M1/70.15) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50. Use reported in scientific literature (PMID 28683563)

Immunocytochemistry/ Immunofluorescence

1:10-1:500. Use reported by customer review

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500. Use reported in scientific literature

Immunohistochemistry-Paraffin

1:10-1:500. Use reported in scientific literature (PMID 28700779)

Immunoprecipitation

1:10-1:500
Application Notes
IHC-P: Perform enzymatic antigen retrieval before commencing with IHC staining protocol. NB600-1327 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue. This antibody is CyTOF ready.

Reviewed Applications

Read 2 reviews rated 5 using NB600-1327 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD11b/Integrin alpha M

CD11b (integrin alpha M subunit) is a type I transmembrane glycoprotein. CD11b combines with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin alpha M/beta 2, also known as Mac-1 and complement receptor type 3 (CR3). The CD11b/CD18 (Mac-1) heterodimer has a theoretical molecular weight of 127kDa but is often observed near 170kDa due to post translational modifications. Integrins are transmembrane proteins that mediate interactions between adhesion molecules on adjacent cells and/or the extracellular matrix. Integrins have diverse roles in several biological processes, including cell migration during development, wound healing, cell differentiation, and apoptosis. The particular integrin CD11b is known as a pro-inflammatory molecule given its ability to promote phagocyte cytotoxic functions while enhancing several effector molecules such as FcGR, uPAR, and CD14 (1). In macrophages, CpG-DNA stimulates lysosomal degradation and vacuolar acidification, subsequently promoting CD11b release via TLR9 (2). During an acute infection, high CD11b/Mac-1 expression on antigen-specific CD8 (+) T cells can signify recent activation of the immune system, whereas naive T cells and virus-specific memory CD8 (+) T cells express little or no Mac-1 when exposed to a virus (3). Variations at the ITGAM gene, which encodes for the CD11b chain of the Mac-1 integrin, is one of the main genetic risk factors involved in systemic lupus erythematosus /SLE disorder (4).

References

1. Rosetti, F., & Mayadas, T. N. (2016). The many faces of Mac-1 in autoimmune disease. Immunol Rev, 269(1), 175-193. doi:10.1111/imr.12373

2. Kim, D., Kim, T. H., Wu, G., Park, B. K., Ha, J. H., Kim, Y. S.,... Kwon, H. J. (2016). Extracellular Release of CD11b by TLR9 Stimulation in Macrophages. PLoS One, 11(3), e0150677. doi:10.1371/journal.pone.0150677

3. Christensen, J. E., Andreasen, S. O., Christensen, J. P., & Thomsen, A. R. (2001). CD11b expression as a marker to distinguish between recently activated effector CD8(+) T cells and memory cells. Int Immunol, 13(4), 593-600. doi:10.1093/intimm/13.4.593

4. Nath, S. K., Han, S., Kim-Howard, X., Kelly, J. A., Viswanathan, P., Gilkeson, G. S.,... Harley, J. B. (2008). A nonsynonymous functional variant in integrin-alpha(M) (encoded by ITGAM) is associated with systemic lupus erythematosus. Nat Genet, 40(2), 152-154. doi:10.1038/ng.71

Alternate Names

CD11b, Integrin alpha M, ITGAM, cd11b flow antibody, M1/70, M1/70 cd11b, m1/70 clone, M1/70.15 Antibody, M1/70.15 CD11b, M1/70.15 CD11b Antibody, M1/70.15 Clone, M1/70.15 Conjugated, M1/70.15 ICC Antibody, M1/70.15 IHC Antibody, M1/70.15 Immunocytochemistry Antibody, M1/70.15 Immunohistochemistry Antibody, M1/70.15 Microglia Marker, M1/70.15 Myeloid Marker

Entrez Gene IDs

3684 (Human); 16409 (Mouse)

Gene Symbol

ITGAM

UniProt

P05555 (Mouse)

Additional CD11b/Integrin alpha M Products

Product Documents for CD11b Antibody (M1/70.15) - BSA Free

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To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD11b Antibody (M1/70.15) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD11b Antibody (M1/70.15) - BSA Free

Customer Reviews for CD11b Antibody (M1/70.15) - BSA Free (2)

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • CD11b Antibody (M1/70.15)
    Name: Lisha Joshi
    Application: Immunocytochemistry
    Sample Tested: Murine primary microglia
    Species: Mouse
    Verified Customer | Posted 06/10/2019
    Cells were treated with LPA or LPA and an LPAR5 inhibitor in order to investigate the expression of iNOS after 24 hours. CD11b antibody was used in a dilution 1:100 in order to stain primary murine microglia.Cy2 (green) and Cy3 (red) were used as secondary antibodies.
    CD11b Antibody (M1/70.15) - BSA Free NB600-1327
  • CD11b Antibody (M1/70.15)
    Name: Joanna Plastira
    Application: Immunocytochemistry
    Sample Tested: Microglia cells
    Species: Mouse
    Verified Customer | Posted 06/07/2019
    CD11b Antibody (M1/70.15) - BSA Free NB600-1327

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD11b Antibody (M1/70.15) - BSA Free

Showing  1 - 5 of 8 FAQs Showing All

  • Q: Do you know if this CD11b antibody can be used in CD11b positive cell depletion? 

    A: This particular CD11b antibody is not reccomended for depletion studies due to the preservative used; however, our CD11b antibody NBP1-06650 is offered in a preservative free format and has been validated for blocking.

  • Q: I am looking for a Mac-1 (microglia cell marker) antibody to do immunostaining with my mouse brain and I checked, you have a lot of antibodies for this biomarker. I don't know which one is the best to be used for immunohistology on mouse brain tissue. Could you please help with this?

    A:

    I would recommend NB110-89474. This antibody has been published in 11 scientific articles, including several times in IHC in mouse. As long as CD11b is expressed in your sample, it should work just fine and is backed by our 100% Novus Guarantee.

  • Q: I'm not sure if this will work for me, can I get a free sample of your CD11b antibody to test?

    A: We don't offer free samples but we do offer this CD11b antibody in a trial size of 0.025 ml at a reduced cost, all antibodies are backed by our 100% guarantee to work in all validated species and applications on the product's web page.

  • Q: Is CD11b antibody also staining neutrophils in the brain

    A: CD11b is typically used as a marker for granulocytes and macrophages. You may need a combination of markers to specifically identify neutrophils

  • Q: Looking for a bone marrow macrophage marker can you recommend a CD11b antibody for ICC?

    A: I would recommend NB110-89474 which is our most popular CD11b antibody and has been validated in ICC and cited in 18 publications.

  • Q: The protocol says to do enzymatic antigen retrieval before incubating with the CD11b antibody, which enzyme is used?

    A: The protocol is referencting the PIER method using Proteinase K as the enzyme.

  • Q: We need a CD11b antibody for IHC-P on mouse tissue conjugated to FITC, do you have one that will work?

    A: We have 3 CD11b antibodies for IHC-P and just one CD11 b antibody we offer conjugated to FITC, NB110-89474F.

  • Q: What types of cells can this CD11b antibody be used as a marker for?

    A: A study used this CD11b antibody to stain granulocytes in mouse uterine tissue in IHC. CD11b is also expressed on other leukocytes such as monocytes, macrophages, and natural killer cells.

  • Q: Do you know if this CD11b antibody can be used in CD11b positive cell depletion? 

    A: This particular CD11b antibody is not reccomended for depletion studies due to the preservative used; however, our CD11b antibody NBP1-06650 is offered in a preservative free format and has been validated for blocking.

  • Q: I am looking for a Mac-1 (microglia cell marker) antibody to do immunostaining with my mouse brain and I checked, you have a lot of antibodies for this biomarker. I don't know which one is the best to be used for immunohistology on mouse brain tissue. Could you please help with this?

    A:

    I would recommend NB110-89474. This antibody has been published in 11 scientific articles, including several times in IHC in mouse. As long as CD11b is expressed in your sample, it should work just fine and is backed by our 100% Novus Guarantee.

  • Q: I'm not sure if this will work for me, can I get a free sample of your CD11b antibody to test?

    A: We don't offer free samples but we do offer this CD11b antibody in a trial size of 0.025 ml at a reduced cost, all antibodies are backed by our 100% guarantee to work in all validated species and applications on the product's web page.

  • Q: Is CD11b antibody also staining neutrophils in the brain

    A: CD11b is typically used as a marker for granulocytes and macrophages. You may need a combination of markers to specifically identify neutrophils

  • Q: Looking for a bone marrow macrophage marker can you recommend a CD11b antibody for ICC?

    A: I would recommend NB110-89474 which is our most popular CD11b antibody and has been validated in ICC and cited in 18 publications.

  • Q: The protocol says to do enzymatic antigen retrieval before incubating with the CD11b antibody, which enzyme is used?

    A: The protocol is referencting the PIER method using Proteinase K as the enzyme.

  • Q: We need a CD11b antibody for IHC-P on mouse tissue conjugated to FITC, do you have one that will work?

    A: We have 3 CD11b antibodies for IHC-P and just one CD11 b antibody we offer conjugated to FITC, NB110-89474F.

  • Q: What types of cells can this CD11b antibody be used as a marker for?

    A: A study used this CD11b antibody to stain granulocytes in mouse uterine tissue in IHC. CD11b is also expressed on other leukocytes such as monocytes, macrophages, and natural killer cells.

  • Q: Do you know if this CD11b antibody can be used in CD11b positive cell depletion? 

    A: This particular CD11b antibody is not reccomended for depletion studies due to the preservative used; however, our CD11b antibody NBP1-06650 is offered in a preservative free format and has been validated for blocking.

  • Q: I am looking for a Mac-1 (microglia cell marker) antibody to do immunostaining with my mouse brain and I checked, you have a lot of antibodies for this biomarker. I don't know which one is the best to be used for immunohistology on mouse brain tissue. Could you please help with this?

    A:

    I would recommend NB110-89474. This antibody has been published in 11 scientific articles, including several times in IHC in mouse. As long as CD11b is expressed in your sample, it should work just fine and is backed by our 100% Novus Guarantee.

  • Q: I'm not sure if this will work for me, can I get a free sample of your CD11b antibody to test?

    A: We don't offer free samples but we do offer this CD11b antibody in a trial size of 0.025 ml at a reduced cost, all antibodies are backed by our 100% guarantee to work in all validated species and applications on the product's web page.

  • Q: Is CD11b antibody also staining neutrophils in the brain

    A: CD11b is typically used as a marker for granulocytes and macrophages. You may need a combination of markers to specifically identify neutrophils

  • Q: Looking for a bone marrow macrophage marker can you recommend a CD11b antibody for ICC?

    A: I would recommend NB110-89474 which is our most popular CD11b antibody and has been validated in ICC and cited in 18 publications.

  • Q: The protocol says to do enzymatic antigen retrieval before incubating with the CD11b antibody, which enzyme is used?

    A: The protocol is referencting the PIER method using Proteinase K as the enzyme.

  • Q: We need a CD11b antibody for IHC-P on mouse tissue conjugated to FITC, do you have one that will work?

    A: We have 3 CD11b antibodies for IHC-P and just one CD11 b antibody we offer conjugated to FITC, NB110-89474F.

  • Q: What types of cells can this CD11b antibody be used as a marker for?

    A: A study used this CD11b antibody to stain granulocytes in mouse uterine tissue in IHC. CD11b is also expressed on other leukocytes such as monocytes, macrophages, and natural killer cells.

  • Q: Do you know if this CD11b antibody can be used in CD11b positive cell depletion? 

    A: This particular CD11b antibody is not reccomended for depletion studies due to the preservative used; however, our CD11b antibody NBP1-06650 is offered in a preservative free format and has been validated for blocking.

  • Q: I am looking for a Mac-1 (microglia cell marker) antibody to do immunostaining with my mouse brain and I checked, you have a lot of antibodies for this biomarker. I don't know which one is the best to be used for immunohistology on mouse brain tissue. Could you please help with this?

    A:

    I would recommend NB110-89474. This antibody has been published in 11 scientific articles, including several times in IHC in mouse. As long as CD11b is expressed in your sample, it should work just fine and is backed by our 100% Novus Guarantee.

  • Q: I'm not sure if this will work for me, can I get a free sample of your CD11b antibody to test?

    A: We don't offer free samples but we do offer this CD11b antibody in a trial size of 0.025 ml at a reduced cost, all antibodies are backed by our 100% guarantee to work in all validated species and applications on the product's web page.

  • Q: Is CD11b antibody also staining neutrophils in the brain

    A: CD11b is typically used as a marker for granulocytes and macrophages. You may need a combination of markers to specifically identify neutrophils

  • Q: Looking for a bone marrow macrophage marker can you recommend a CD11b antibody for ICC?

    A: I would recommend NB110-89474 which is our most popular CD11b antibody and has been validated in ICC and cited in 18 publications.

  • Q: The protocol says to do enzymatic antigen retrieval before incubating with the CD11b antibody, which enzyme is used?

    A: The protocol is referencting the PIER method using Proteinase K as the enzyme.

  • Q: We need a CD11b antibody for IHC-P on mouse tissue conjugated to FITC, do you have one that will work?

    A: We have 3 CD11b antibodies for IHC-P and just one CD11 b antibody we offer conjugated to FITC, NB110-89474F.

  • Q: What types of cells can this CD11b antibody be used as a marker for?

    A: A study used this CD11b antibody to stain granulocytes in mouse uterine tissue in IHC. CD11b is also expressed on other leukocytes such as monocytes, macrophages, and natural killer cells.

  • Q: Do you know if this CD11b antibody can be used in CD11b positive cell depletion? 

    A: This particular CD11b antibody is not reccomended for depletion studies due to the preservative used; however, our CD11b antibody NBP1-06650 is offered in a preservative free format and has been validated for blocking.

  • Q: I am looking for a Mac-1 (microglia cell marker) antibody to do immunostaining with my mouse brain and I checked, you have a lot of antibodies for this biomarker. I don't know which one is the best to be used for immunohistology on mouse brain tissue. Could you please help with this?

    A:

    I would recommend NB110-89474. This antibody has been published in 11 scientific articles, including several times in IHC in mouse. As long as CD11b is expressed in your sample, it should work just fine and is backed by our 100% Novus Guarantee.

  • Q: I'm not sure if this will work for me, can I get a free sample of your CD11b antibody to test?

    A: We don't offer free samples but we do offer this CD11b antibody in a trial size of 0.025 ml at a reduced cost, all antibodies are backed by our 100% guarantee to work in all validated species and applications on the product's web page.

  • Q: Is CD11b antibody also staining neutrophils in the brain

    A: CD11b is typically used as a marker for granulocytes and macrophages. You may need a combination of markers to specifically identify neutrophils

  • Q: Looking for a bone marrow macrophage marker can you recommend a CD11b antibody for ICC?

    A: I would recommend NB110-89474 which is our most popular CD11b antibody and has been validated in ICC and cited in 18 publications.

  • Q: The protocol says to do enzymatic antigen retrieval before incubating with the CD11b antibody, which enzyme is used?

    A: The protocol is referencting the PIER method using Proteinase K as the enzyme.

  • Q: We need a CD11b antibody for IHC-P on mouse tissue conjugated to FITC, do you have one that will work?

    A: We have 3 CD11b antibodies for IHC-P and just one CD11 b antibody we offer conjugated to FITC, NB110-89474F.

  • Q: What types of cells can this CD11b antibody be used as a marker for?

    A: A study used this CD11b antibody to stain granulocytes in mouse uterine tissue in IHC. CD11b is also expressed on other leukocytes such as monocytes, macrophages, and natural killer cells.

  • Q: Do you know if this CD11b antibody can be used in CD11b positive cell depletion? 

    A: This particular CD11b antibody is not reccomended for depletion studies due to the preservative used; however, our CD11b antibody NBP1-06650 is offered in a preservative free format and has been validated for blocking.

  • Q: I am looking for a Mac-1 (microglia cell marker) antibody to do immunostaining with my mouse brain and I checked, you have a lot of antibodies for this biomarker. I don't know which one is the best to be used for immunohistology on mouse brain tissue. Could you please help with this?

    A:

    I would recommend NB110-89474. This antibody has been published in 11 scientific articles, including several times in IHC in mouse. As long as CD11b is expressed in your sample, it should work just fine and is backed by our 100% Novus Guarantee.

  • Q: I'm not sure if this will work for me, can I get a free sample of your CD11b antibody to test?

    A: We don't offer free samples but we do offer this CD11b antibody in a trial size of 0.025 ml at a reduced cost, all antibodies are backed by our 100% guarantee to work in all validated species and applications on the product's web page.

  • Q: Is CD11b antibody also staining neutrophils in the brain

    A: CD11b is typically used as a marker for granulocytes and macrophages. You may need a combination of markers to specifically identify neutrophils

  • Q: Looking for a bone marrow macrophage marker can you recommend a CD11b antibody for ICC?

    A: I would recommend NB110-89474 which is our most popular CD11b antibody and has been validated in ICC and cited in 18 publications.

  • Q: The protocol says to do enzymatic antigen retrieval before incubating with the CD11b antibody, which enzyme is used?

    A: The protocol is referencting the PIER method using Proteinase K as the enzyme.

  • Q: We need a CD11b antibody for IHC-P on mouse tissue conjugated to FITC, do you have one that will work?

    A: We have 3 CD11b antibodies for IHC-P and just one CD11 b antibody we offer conjugated to FITC, NB110-89474F.

  • Q: What types of cells can this CD11b antibody be used as a marker for?

    A: A study used this CD11b antibody to stain granulocytes in mouse uterine tissue in IHC. CD11b is also expressed on other leukocytes such as monocytes, macrophages, and natural killer cells.

  • Q: Do you know if this CD11b antibody can be used in CD11b positive cell depletion? 

    A: This particular CD11b antibody is not reccomended for depletion studies due to the preservative used; however, our CD11b antibody NBP1-06650 is offered in a preservative free format and has been validated for blocking.

  • Q: I am looking for a Mac-1 (microglia cell marker) antibody to do immunostaining with my mouse brain and I checked, you have a lot of antibodies for this biomarker. I don't know which one is the best to be used for immunohistology on mouse brain tissue. Could you please help with this?

    A:

    I would recommend NB110-89474. This antibody has been published in 11 scientific articles, including several times in IHC in mouse. As long as CD11b is expressed in your sample, it should work just fine and is backed by our 100% Novus Guarantee.

  • Q: I'm not sure if this will work for me, can I get a free sample of your CD11b antibody to test?

    A: We don't offer free samples but we do offer this CD11b antibody in a trial size of 0.025 ml at a reduced cost, all antibodies are backed by our 100% guarantee to work in all validated species and applications on the product's web page.

  • Q: Is CD11b antibody also staining neutrophils in the brain

    A: CD11b is typically used as a marker for granulocytes and macrophages. You may need a combination of markers to specifically identify neutrophils

  • Q: Looking for a bone marrow macrophage marker can you recommend a CD11b antibody for ICC?

    A: I would recommend NB110-89474 which is our most popular CD11b antibody and has been validated in ICC and cited in 18 publications.

  • Q: The protocol says to do enzymatic antigen retrieval before incubating with the CD11b antibody, which enzyme is used?

    A: The protocol is referencting the PIER method using Proteinase K as the enzyme.

  • Q: We need a CD11b antibody for IHC-P on mouse tissue conjugated to FITC, do you have one that will work?

    A: We have 3 CD11b antibodies for IHC-P and just one CD11 b antibody we offer conjugated to FITC, NB110-89474F.

  • Q: What types of cells can this CD11b antibody be used as a marker for?

    A: A study used this CD11b antibody to stain granulocytes in mouse uterine tissue in IHC. CD11b is also expressed on other leukocytes such as monocytes, macrophages, and natural killer cells.

  • Q: Do you know if this CD11b antibody can be used in CD11b positive cell depletion? 

    A: This particular CD11b antibody is not reccomended for depletion studies due to the preservative used; however, our CD11b antibody NBP1-06650 is offered in a preservative free format and has been validated for blocking.

  • Q: I am looking for a Mac-1 (microglia cell marker) antibody to do immunostaining with my mouse brain and I checked, you have a lot of antibodies for this biomarker. I don't know which one is the best to be used for immunohistology on mouse brain tissue. Could you please help with this?

    A:

    I would recommend NB110-89474. This antibody has been published in 11 scientific articles, including several times in IHC in mouse. As long as CD11b is expressed in your sample, it should work just fine and is backed by our 100% Novus Guarantee.

  • Q: I'm not sure if this will work for me, can I get a free sample of your CD11b antibody to test?

    A: We don't offer free samples but we do offer this CD11b antibody in a trial size of 0.025 ml at a reduced cost, all antibodies are backed by our 100% guarantee to work in all validated species and applications on the product's web page.

  • Q: Is CD11b antibody also staining neutrophils in the brain

    A: CD11b is typically used as a marker for granulocytes and macrophages. You may need a combination of markers to specifically identify neutrophils

  • Q: Looking for a bone marrow macrophage marker can you recommend a CD11b antibody for ICC?

    A: I would recommend NB110-89474 which is our most popular CD11b antibody and has been validated in ICC and cited in 18 publications.

  • Q: The protocol says to do enzymatic antigen retrieval before incubating with the CD11b antibody, which enzyme is used?

    A: The protocol is referencting the PIER method using Proteinase K as the enzyme.

  • Q: We need a CD11b antibody for IHC-P on mouse tissue conjugated to FITC, do you have one that will work?

    A: We have 3 CD11b antibodies for IHC-P and just one CD11 b antibody we offer conjugated to FITC, NB110-89474F.

  • Q: What types of cells can this CD11b antibody be used as a marker for?

    A: A study used this CD11b antibody to stain granulocytes in mouse uterine tissue in IHC. CD11b is also expressed on other leukocytes such as monocytes, macrophages, and natural killer cells.

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