CD14 Antibody (M5E2) [Alexa Fluor® 488]

Novus Biologicals | Catalog # NB100-77758AF488

Clone M5E2 was used by HLDA to establish CD designation.
Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Porcine, Bovine, Canine, Primate

Cited:

Porcine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Block/Neutralize, Flow Cytometry, Dual RNAscope ISH-IHC, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # M5E2
View all CD14 Primary Antibodies »

Product Specifications

Immunogen

Human peripheral blood cells.

Reactivity Notes

Expected to cross react with Chimpanzee, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, Cotton-topped Tamarin, Common Marmoset, Squirrel Monkey.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for CD14 Antibody (M5E2) [Alexa Fluor® 488]

Immunohistochemistry-Frozen: CD14 Antibody (M5E2) [Alexa Fluor 488] [NB100-77758AF488] - Human lung tissue was stained with FITC-conjugated CD14 (Green) for overnight.
Flow Cytometry: CD14 Antibody (M5E2) [Alexa Fluor 488] [NB100-77758AF488] - A surface stain was performed on human peripheral blood monocytes with CD163 (EDHu-1) antibody NB110-40686APC and a matched isotype control NBP2-27287APC. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. A co-stain was performed with NB100-77758AF488.

Applications for CD14 Antibody (M5E2) [Alexa Fluor® 488]

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Dual RNAscope ISH-IHC

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4 using NB100-77758AF488 in the following applications:

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD14

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils. The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS.

Alternate Names

CD14

Gene Symbol

CD14

Additional CD14 Products

Product Documents for CD14 Antibody (M5E2) [Alexa Fluor® 488]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD14 Antibody (M5E2) [Alexa Fluor® 488]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD14 Antibody (M5E2) [Alexa Fluor® 488]

Customer Reviews for CD14 Antibody (M5E2) [Alexa Fluor® 488] (2)

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2 Customer Ratings
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Customer Images

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  • CD14 Antibody (M5E2) [Alexa Fluor® 488]
    Name: James Xiao
    Application: Flow Cytometry
    Sample Tested: Mouse spleen cells
    Species: Mouse
    Verified Customer | Posted 01/26/2018
    Solid red line: CD14-Alexa488 Solid gray area: isotype control ** This is a conformational study to demonstrate that this clone (M5E2) of CD14 antibody did not react with mouse splenocytes.
    Dilution: 1:100 in FACS buffer.
    CD14 Antibody (M5E2) [Alexa Fluor® 488] NB100-77758AF488
  • CD14 Antibody (M5E2) [Alexa Fluor® 488]
    Name: James Xiao
    Application: Immunohistochemistry-Frozen
    Sample Tested: Adult lung
    Species: Human
    Verified Customer | Posted 01/03/2018
    Human lung tissue was stained with FITC-conjugated CD14 (Green) for overnight.
    CD14 Antibody (M5E2) [Alexa Fluor® 488] NB100-77758AF488

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