CD14 Antibody (M5E2) [Alexa Fluor® 700]

Novus Biologicals | Catalog # NB100-77758AF700

Clone M5E2 was used by HLDA to establish CD designation.
Novus Biologicals

Key Product Details

Species Reactivity

Human, Porcine, Bovine, Canine, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Block/Neutralize, Flow Cytometry, Dual RNAscope ISH-IHC, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # M5E2
View all CD14 Primary Antibodies »

Product Specifications

Immunogen

Human peripheral blood cells.

Reactivity Notes

Expected to cross react with Chimpanzee, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, Cotton-topped Tamarin, Common Marmoset, Squirrel Monkey.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for CD14 Antibody (M5E2) [Alexa Fluor® 700]

Product Image: CD14 Antibody (M5E2) [Alexa Fluor® 700] [NB100-77758AF700] - Vial of Alexa Fluor 700 conjugated antibody. Alexa Fluor 700 is optimally excited at 696 nm by the Red laser (633 or 640 nm) and has an emission maximum of 720 nm.

Applications for CD14 Antibody (M5E2) [Alexa Fluor® 700]

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Dual RNAscope ISH-IHC

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4 using NB100-77758AF700 in the following applications:

Spectra Viewer

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD14

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils. The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS.

Alternate Names

CD14

Gene Symbol

CD14

Additional CD14 Products

Product Documents for CD14 Antibody (M5E2) [Alexa Fluor® 700]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CD14 Antibody (M5E2) [Alexa Fluor® 700]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for CD14 Antibody (M5E2) [Alexa Fluor® 700] (2)

4 out of 5
2 Customer Ratings
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  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Human PBMC
    Species: Human
    Verified Customer | Posted 11/18/2014
    CD14 Antibody (M5E2) [Alexa Fluor® 700] NB100-77758AF700
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Human PBMC
    Species: Human
    Verified Customer | Posted 11/18/2014
    human PBMC
    CD14 Antibody (M5E2) [Alexa Fluor® 700] NB100-77758AF700

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