CD14 Antibody (M5E2) - Azide and BSA Free

Name: CD14 Antibody (M5E2) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP2-80627
Price: 279 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-80627

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Product Documents

Clone M5E2 was used by HLDA to establish CD designation.

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Porcine, Bovine, Canine, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # M5E2

Format

Azide and BSA Free

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Product Specifications

Immunogen

Human peripheral blood cells.

Reactivity Notes

Expected to cross react with Chimpanzee, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, Cotton-topped Tamarin, Common Marmoset, Squirrel Monkey.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for CD14 Antibody (M5E2) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627]

Immunocytochemistry/Immunofluorescence: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627] - Representative immunostaining of CD14 (red) in adult human lung tissue sample with nuclei counterstained blue. This image was submitted by customer review. Image from the Alexa Fluor 647 version of this antibody. Image from the standard format of this antibody.

Flow Cytometry: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627]

Flow Cytometry: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627] - Analysis using the PE conjugate of NB100-77758. Staining of CD14 in human PBMC using anti-CD14 antibody conjugated with PE. Image from verified customer review.

Immunocytochemistry/ Immunofluorescence: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627]

Immunocytochemistry/Immunofluorescence: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627] - Human lung tissue was stained with FITC-conjugated CD14 (Green) for overnight. Image from the Alexa Fluor 488 version of this antibody.

Flow Cytometry: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627]

Flow Cytometry: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627] - A surface stain was performed on human peripheral blood monocytes with CD163 (EDHu-1) antibody NB110-40686APC and a matched isotype control NBP2-27287APC. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. A co-stain was performed with NB100-77758AF488. Image from the standard format of this antibody.

Flow Cytometry: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627]

Flow Cytometry: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627] - Analysis using PE conjugate of NB100-77758. A cell surface stain was performed on hPBMCs with ILT5 antibody (MM0413-9S32) NBP2-11729 (top image) and a matched isotype control NBP2-27287 (bottom image). Cells were incubated in an antibody dilution of 1:200 for 20 minutes at room temperature. Both antibodies were directly conjugated to APC. A co-stain was performed using CD14 antibody (M5E2) NB100-77758PE.

Flow Cytometry: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627]

Flow Cytometry: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627] - Analysis using the FITC conjugate of NB100-77758. Staining of Human peripheral blood monocytes with M5E2 FITC.

Flow Cytometry: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627]

Flow Cytometry: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627] - Human peripheral blood monocytes stained with M5E2 APC Image from the standard format of this antibody.

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Applications for CD14 Antibody (M5E2) - Azide and BSA Free

Application

Recommended Usage

Block/Neutralize

reported in scientific literature (PMID 27228163)

Flow Cytometry

2.0 ug/10^6 cells in 100 ul

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:10 - 1:500

Application Notes

The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation, and immunofluorescence microscopy. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. This antibody is CyTOF ready.

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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD14

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils. The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS.

Alternate Names

CD14

Gene Symbol

CD14

Additional CD14 Products

Product Documents for CD14 Antibody (M5E2) - Azide and BSA Free

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Product Specific Notices for CD14 Antibody (M5E2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.