CD14 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86168

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CD14 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PCELDDEDFRCVCNFSEPQPDWSEAFQCVSAVEVEIHAGGLNLEPFLKRVDADADPRQYADTVKALRVRRLTVGAAQVPAQLLVGALRVLAYSRLKELTLEDLKITGTMPPLPLEATGLALSSL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit CD14 Antibody - BSA Free (NBP1-86168) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-CD14 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CD14 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CD14 Antibody [NBP1-86168]

Immunocytochemistry/ Immunofluorescence: CD14 Antibody [NBP1-86168]

Immunocytochemistry/Immunofluorescence: CD14 Antibody [NBP1-86168] - Staining of human cell line SiHa shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168]

Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168]

Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Analysis in human lymph node and pancreas tissues. Corresponding CD14 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168]

Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168]

Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Staining of human liver, lymph node, pancreas and rectum using Anti-CD14 antibody NBP1-86168 (A) shows similar protein distribution across tissues to independent antibody NBP1-86166 (B).
Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168]

Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168]

Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Staining of human tonsil shows moderate membranous positivity in germinal center cells.
Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168]

Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168]

Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Staining of human rectum shows moderate to strong cytoplasmic positivity in lymphoid cells.
Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168]

Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168]

Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Staining of human pancreas shows no cytoplasmic positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168]

Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168]

Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Staining of human lymph node shows moderate to strong membranous positivity in lymphoid cells.
Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168]

Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168]

Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Staining of human liver shows moderate to strong membranous positivity in hepatic sinusoid cells.
CD14 Antibody - BSA Free Western Blot: CD14 Antibody - BSA Free [NBP1-86168]

Western Blot: CD14 Antibody - BSA Free [NBP1-86168]

Analysis using Anti-CD14 antibody NBP1-86168 (A) shows similar pattern to independent antibody NBP1-86166 (B).

Applications for CD14 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD14

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils. The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS.

Alternate Names

CD14

Gene Symbol

CD14

Additional CD14 Products

Product Documents for CD14 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CD14 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD14 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD14 Antibody - BSA Free

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    • Q: I am interested in your anti-human CD14 (NBP1-86168). What concentration does this antibodies has? Does it come in carrier-free form (without protein stabilizer/BSA)?
      A: NBP1-86168 is supplied in PBS (pH 7.2) with 40% glycerol and 0.02% sodium azide. The current lot has a concentration of 0.2mg/ml.
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