CD25/IL-2R alpha Antibody (BLR157J)

Name: CD25/IL-2R alpha Antibody (BLR157J)
Brand: Novus Biologicals
SKU: NBP3-14733
Price: 519 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-14733

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
Related Blogs

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR157J

View all CD25/IL-2R alpha Primary Antibodies »

Product Specifications

Immunogen

residues 22-213 (ECD)

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for CD25/IL-2R alpha Antibody (BLR157J)

Immunoprecipitation: CD25/IL-2R alpha Antibody (BLR157J) [NBP3-14733]

Immunoprecipitation: CD25/IL-2R alpha Antibody [NBP3-14733] - Whole cell lysate (0.5 mgper IP reaction; 5% of IP loaded) from MJ cells preparedusing NETN lysis buffer. Antibodies: Rabbit antiCD25/IL-2R alpha recombinant monoclonal antibody used for IP at 20 ul/mg lysate.CD25/IL-2R alpha was also immunoprecipitated by anotherantibody against a different epitope of CD25/IL-2R alpha. For blotting immunoprecipitatedCD25/IL-2R alpha, was used at 1:1000.Chemiluminescence with an exposure time of 30 seconds.

Applications for CD25/IL-2R alpha Antibody (BLR157J)

Application

Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: CD25/IL-2R alpha

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.(provided by RefSeq)

Long Name

Interleukin 2 Receptor alpha

Alternate Names

CD25, IL-2 R alpha, IL-2Ra, IL2R alpha, IL2RA

Gene Symbol

IL2RA

Additional CD25/IL-2R alpha Products

Product Documents for CD25/IL-2R alpha Antibody (BLR157J)

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Certificate of Analysis

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Product Specific Notices for CD25/IL-2R alpha Antibody (BLR157J)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.