Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bovine, Goat, Rabbit, Sheep

Cited:

Human, Mouse, Rabbit

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA, Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Cited:

Immunohistochemistry-Paraffin, Western Blot, Block/Neutralize, Flow Cytometry, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # Hermes-1

Format

Azide and BSA Free
View all CD44 Primary Antibodies »

Product Specifications

Immunogen

80 - 95 kDa lymphocyte surface glycoprotein H-CAM (CD44).

Marker

Cell Membrane Marker

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for CD44 Antibody (Hermes-1) - Azide and BSA Free

Western Blot: CD44 Antibody (Hermes-1)Azide and BSA Free [NBP2-22530]

Western Blot: CD44 Antibody (Hermes-1)Azide and BSA Free [NBP2-22530]

Western Blot: CD44 Antibody (Hermes-1) [NBP2-22530] - of Hela cell lysates.
Immunocytochemistry/ Immunofluorescence: CD44 Antibody (Hermes-1) - Azide and BSA Free [NBP2-22530]

Immunocytochemistry/ Immunofluorescence: CD44 Antibody (Hermes-1) - Azide and BSA Free [NBP2-22530]

Immunocytochemistry/Immunofluorescence: CD44 Antibody (Hermes-1) [NBP2-22530] - Analysis of CD44 (green) showing staining in the membrane of Hela cells (right) compared to a negative control without primary antibody (left).
Flow Cytometry: CD44 Antibody (Hermes-1) - Azide and BSA Free [NBP2-22530]

Flow Cytometry: CD44 Antibody (Hermes-1) - Azide and BSA Free [NBP2-22530]

Flow Cytometry: CD44 Antibody (Hermes-1) [NBP2-22530] - A surface stain was performed on U-87 cells with CD44 (Hermes-1) Antibody NBP2-22530R (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to DyLight 550.
Flow Cytometry: CD44 Antibody (Hermes-1) - Azide and BSA Free [NBP2-22530]

Flow Cytometry: CD44 Antibody (Hermes-1) - Azide and BSA Free [NBP2-22530]

Flow Cytometry: CD44 Antibody (Hermes-1) [NBP2-22530] - Analysis of CD44 in PBMC cells (green) compared to an isotype control (blue).
Flow Cytometry: CD44 Antibody (Hermes-1)Azide and BSA Free [NBP2-22530]

Flow Cytometry: CD44 Antibody (Hermes-1)Azide and BSA Free [NBP2-22530]

Flow Cytometry: CD44 Antibody (Hermes-1) [NBP2-22530] - A surface stain was performed on U-87 cells with CD44 [Hermes-1] Antibody NBP2-22530AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Applications for CD44 Antibody (Hermes-1) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

1:10 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:200

Immunohistochemistry

1:10 - 1:2000

Immunoprecipitation

1:10 - 1:50

Western Blot

1:1 - 1:100

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CD44

CD44 represents a family of type I transmembrane glycoproteins expressed on many cell types such as B cells, monocytes, macrophages, and variable subsets of thymocytes and peripheral T cells. CD44 primarily functions as an adhesion molecule. It has been implicated in various processes, such as hematopoiesis, lymphocyte homing, leukocyte activation, tumor metastasis and development. The KM201 monoclonal antibody reacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule. KM201 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and Whitlock-Witte Cultures, prevent the earliest intrathymic precursors from homing to the thymus, and costimulate the activation of freshly purified splenic CD4+ T cells.1-6

Alternate Names

CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1

Entrez Gene IDs

960 (Human)

Gene Symbol

CD44

UniProt

P16070

Additional CD44 Products

Product Documents for CD44 Antibody (Hermes-1) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CD44 Antibody (Hermes-1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD44 Antibody (Hermes-1) - Azide and BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD44 Antibody (Hermes-1) - Azide and BSA Free

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  • Q: I need to do a neutralization assay with this Antibody in cell culture. Could you send me the best concentration to use?

    A: Although we give recommended dilutions for the other applications in which this antibody is validated, the dilution used for neutralization is assay dependent, and you will need to determine this yourself. Cell type, seeding density and the assay set up will all play a part in deciding this concentration. We recommend that you titrate the antibody for use in your experiment using appropriate negative and positive controls.

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