CD44 Antibody (IM7) - BSA Free

Novus Biologicals | Catalog # NBP1-41266

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Canine, Equine, Feline

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # IM7

Format

BSA Free
View all CD44 Primary Antibodies »

Product Specifications

Immunogen

Dexamethasone-induced cells of the SJL mouse spontaneous myeloid leukemia M1

Reactivity Notes

Cat (100%). Canine reactivity reported from a verified customer review.

Marker

Cell Membrane Marker

Specificity

The rat monoclonal antibody IM7 reacts with CD44 antigen (Phagocyte glycoprotein 1), an 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes. The antibody reacts with all isoforms of mouse CD44.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Theoretical MW

95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD44 Antibody (IM7) - BSA Free

Western Blot: CD44 Antibody (IM7)BSA Free [NBP1-41266]

Western Blot: CD44 Antibody (IM7)BSA Free [NBP1-41266]

Western Blot: CD44 Antibody (IM7) [NBP1-41266] - Analysis of CD44 in HeLa cells (positive; lane 2) and MOLT-4 cells (negative; lane 1) using anti-CD44 (IM7) purified; non-reducing conditions.
Flow Cytometry: CD44 Antibody (IM7) - BSA Free [NBP1-41266]

Flow Cytometry: CD44 Antibody (IM7) - BSA Free [NBP1-41266]

Flow Cytometry: CD44 Antibody (IM7) [NBP1-41266] - Detection of T-cells in canine whole blood. Image from verified customer review.
CD44 Antibody (IM7) - BSA Free Western Blot: CD44 Antibody (IM7) - BSA Free [NBP1-41266]

Western Blot: CD44 Antibody (IM7) - BSA Free [NBP1-41266]

Analysis of CD44 in HeLa cells (positive; lane 2) and MOLT-4 cells (negative; lane 1) using anti-CD44 (IM7) purified; non-reducing conditions.

Applications for CD44 Antibody (IM7) - BSA Free

Application
Recommended Usage

Flow Cytometry

1 ug/ml

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:200

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:50

Western Blot

1:1000
Application Notes
This antibody is CyTOF ready.

Reviewed Applications

Read 1 review rated 5 using NBP1-41266 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD44

CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.

Alternate Names

CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1

Gene Symbol

CD44

Additional CD44 Products

Product Documents for CD44 Antibody (IM7) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD44 Antibody (IM7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD44 Antibody (IM7) - BSA Free

Customer Reviews for CD44 Antibody (IM7) - BSA Free (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
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3 Stars
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1 Stars
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Customer Images

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  • CD44 Antibody (IM7)
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Whole blood cells
    Species: Canine
    Verified Customer | Posted 05/18/2018
    Detection of T-cells in Dog Blood.
    The whole blood cells were lysed to get rid of the RBC. The washed cell were then incubated with the Antibody. They were washed and then incubated with a conjugated secondary. Allowed to sit for 20mins and then washed again and read on the BD Fortessa.
    CD44 Antibody (IM7) - BSA Free NBP1-41266

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