CD44 Antibody (IM7) [FITC]

Novus Biologicals | Catalog # NBP1-41266F

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Canine, Equine, Feline

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Cited:

Flow Cytometry

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # IM7
View all CD44 Primary Antibodies »

Product Specifications

Immunogen

Dexamethasone-induced cells of the SJL mouse spontaneous myeloid leukemia M1

Reactivity Notes

Cat (100%). Canine reactivity reported from a verified customer review.

Marker

Cell Membrane Marker

Specificity

The rat monoclonal antibody IM7 reacts with CD44 antigen (Phagocyte glycoprotein 1), an 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes. The antibody reacts with all isoforms of mouse CD44.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for CD44 Antibody (IM7) [FITC]

Flow Cytometry: CD44 Antibody (IM7) [FITC] [NBP1-41266F] - Staining of C57BL/6 splenocytes with 0.25 ug of Rat IgG2b kappa Isotype Control FITC (cat. 11-4031) (open histogram) or 0.25 ug of Anti-Human/Mouse CD44 FITC (filled histogram). Total viable cells were used for analysis.

Applications for CD44 Antibody (IM7) [FITC]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP1-41266F in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD44

CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.

Alternate Names

CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1

Gene Symbol

CD44

Additional CD44 Products

Product Documents for CD44 Antibody (IM7) [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CD44 Antibody (IM7) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD44 Antibody (IM7) [FITC]

Customer Reviews for CD44 Antibody (IM7) [FITC] (1)

5 out of 5
1 Customer Rating
5 Stars
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4 Stars
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1 Stars
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Customer Images

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  • CD44 Antibody (IM7) [FITC]
    Name: A G
    Application: Flow Cytometry
    Sample Tested: Dog whole blood
    Species: Canine
    Verified Customer | Posted 03/24/2017
    CD44 Antibody (IM7) [FITC] NBP1-41266F

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FAQs

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