CD44 Antibody (MEM-263) - BSA Free

Novus Biologicals | Catalog # NB500-481

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Porcine, Canine

Cited:

Human, Porcine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Cited:

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MEM-263

Format

BSA Free
View all CD44 Primary Antibodies »

Product Specifications

Immunogen

COS-7 cells, African Green Monkey (NM_000610.3).

Marker

Cell Membrane Marker

Specificity

The antibody MEM-263 reacts with extracellular (N-terminal) domain of standard CD44 (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes. HLDA III; WS Code T 155

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD44 Antibody (MEM-263) - BSA Free

Immunocytochemistry/ Immunofluorescence: CD44 Antibody (MEM-263) - BSA Free [NB500-481]

Immunocytochemistry/ Immunofluorescence: CD44 Antibody (MEM-263) - BSA Free [NB500-481]

Immunocytochemistry/Immunofluorescence: CD44 Antibody (MEM-263) [NB500-481] - analysis of CD44 in 4% paraformaldehyde fixed Caov-3 cells (adherent human ovarian cancer cells) using anti-Cd44 antibody. The primary antibody was used at a dilution of 1:1000 in 2% goat serum in PBS and incubated for 2 hours at room temperature. Image from verified customer review.
CD44 Antibody (MEM-263) [NB500-481] - Analysis of CD44 in HeLa cells using anti-CD44 (MEM-263) purified; non-reducing conditions.
Flow Cytometry: CD44 Antibody (MEM-263) - BSA Free [NB500-481]

Flow Cytometry: CD44 Antibody (MEM-263) - BSA Free [NB500-481]

Flow Cytometry: CD44 Antibody (MEM-263) [NB500-481] - A surface stain was performed on hPBMCs with CD44 (MEM-263) antibody NB500-481AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Flow Cytometry: CD44 Antibody (MEM-263) - BSA Free [NB500-481]

Flow Cytometry: CD44 Antibody (MEM-263) - BSA Free [NB500-481]

Flow Cytometry: CD44 Antibody (MEM-263) [NB500-481] - Staining of human peripheral blood using anti-CD44 (MEM-263) APC conjugate.
Western Blot: CD44 Antibody (MEM-263)BSA Free [NB500-481]

Western Blot: CD44 Antibody (MEM-263)BSA Free [NB500-481]

CD44-Antibody-MEM-263-Western-Blot-NB500-481-img0008.jpg
Western Blot: CD44 Antibody (MEM-263)BSA Free [NB500-481]

Western Blot: CD44 Antibody (MEM-263)BSA Free [NB500-481]

Western Blot: CD44 Antibody (MEM-263) [NB500-481] - Fig. 1. Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-CD44 (MEM-263). Lane 1: lysate of human PBL Lane 2: lysate of canine PBL Lane 3: lysate of porcine PBL.
Flow Cytometry: CD44 Antibody (MEM-263) - BSA Free [NB500-481]

Flow Cytometry: CD44 Antibody (MEM-263) - BSA Free [NB500-481]

Flow Cytometry: CD44 Antibody (MEM-263) [NB500-481] - Analysis of human peripheral blood (lymphocyte gate) using anti-CD44 (MEM-263) PE conjugate.
Flow Cytometry: CD44 Antibody (MEM-263) - BSA Free [NB500-481]

Flow Cytometry: CD44 Antibody (MEM-263) - BSA Free [NB500-481]

Flow Cytometry: CD44 Antibody (MEM-263) [NB500-481] - Staining of human peripheral blood using anti-CD44 (MEM-263) FITC conjugate.
CD44 Antibody (MEM-263) - BSA Free

Western Blot: CD44 Antibody (MEM-263) - BSA Free [NB500-481] -

Expression of CD44 and CK18 in multiple clones of ovarian cancer cells. (a)-(b). CD44+ cells also express CK18 as determined by flow cytometry (a) and western blot analysis (b). Flow cytometer is representative of the eight evaluated clones. EOC stem cells, determined by either CD44 (c and d) or Ck18 (e and f) expression, are found in clusters surrounded by CD44− or Ck18− negative cancer cells.

Applications for CD44 Antibody (MEM-263) - BSA Free

Application
Recommended Usage

Flow Cytometry

4 ug/ml

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

10 ug/ml

Immunoprecipitation

1:50

Western Blot

2 ug/ml
Application Notes
Western Blot - Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (6% separating gel). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 25481699). This antibody is CyTOF ready.

Reviewed Applications

Read 1 review rated 4 using NB500-481 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD44

CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein 1 (pgp 1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.

Alternate Names

CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1

Entrez Gene IDs

960 (Human)

Gene Symbol

CD44

UniProt

P16070

Additional CD44 Products

Product Documents for CD44 Antibody (MEM-263) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD44 Antibody (MEM-263) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD44 Antibody (MEM-263) - BSA Free

Customer Reviews for CD44 Antibody (MEM-263) - BSA Free (1)

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Customer Images

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  • Name: Joelle Sacks
    Application: Immunofluorescence
    Sample Tested: adherent human ovarian cancer cells
    Species: Human
    Verified Customer | Posted 12/16/2014
    Caov-3 ovarian carcinoma cells
    CD44 Antibody (MEM-263) - BSA Free NB500-481

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