CD44 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84578

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CD44 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DDVSSGSSSERSSTSGGYIFYTFSTVHPIPDEDSPWITDSTDRIPATTLMSTSATATETATKRQETWDWFSWLFLPSESKNHLHTTTQMAGTSSNTISAGWEPNEENEDERDRHLSFSGSGIDDDEDFISSTISTTPR

Marker

Cell Membrane Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD44 Antibody - BSA Free

Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578]

Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578]

Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining in human skin and skeletal muscle tissues. Corresponding CD44 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: CD44 Antibody [NBP1-84578]

Immunocytochemistry/ Immunofluorescence: CD44 Antibody [NBP1-84578]

Immunocytochemistry/Immunofluorescence: CD44 Antibody [NBP1-84578] - Staining of human cell line U-251 MG shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578]

Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578]

Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining of human lymph node shows strong positivity in plasma membrane in non-germinal center cells.
Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578]

Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578]

Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining of human skin shows strong membranous positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578]

Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578]

Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining of human squamous cell lung carcinoma shows strong membranous positivity in tumor cells.
Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578]

Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578]

Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.
CD44 Antibody - BSA Free Western Blot: CD44 Antibody - BSA Free [NBP1-84578]

Western Blot: CD44 Antibody - BSA Free [NBP1-84578]

Analysis in human cell lines U-251MG and MCF-7 using Anti-CD44 antibody. Corresponding CD44 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Applications for CD44 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 4 using NBP1-84578 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD44

CD44 represents a family of type I transmembrane glycoproteins expressed on many cell types such as B cells, monocytes, macrophages, and variable subsets of thymocytes and peripheral T cells. CD44 primarily functions as an adhesion molecule. It has been implicated in various processes, such as hematopoiesis, lymphocyte homing, leukocyte activation, tumor metastasis and development. The KM201 monoclonal antibody reacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule. KM201 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and Whitlock-Witte Cultures, prevent the earliest intrathymic precursors from homing to the thymus, and costimulate the activation of freshly purified splenic CD4+ T cells.1-6

Alternate Names

CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1

Gene Symbol

CD44

Additional CD44 Products

Product Documents for CD44 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD44 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD44 Antibody - BSA Free

Customer Reviews for CD44 Antibody - BSA Free (1)

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  • CD44 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: human skin
    Species: Human
    Verified Customer | Posted 04/11/2019
    CD44 Antibody - BSA Free NBP1-84578

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Protocols

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FAQs

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