CD90/Thy1 Antibody (OX-7) - BSA Free

Novus Biologicals | Catalog # NB100-65543

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry Free-Floating, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OX-7

Format

BSA Free
View all CD90/Thy1 Primary Antibodies »

Product Specifications

Immunogen

Rat Thy 1 antigen

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:28928774).

Marker

Mesenchymal Cell Marker

Specificity

This antibody is specific for CD90.1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD90/Thy1 Antibody (OX-7) - BSA Free

Flow Cytometry: CD90/Thy1 Antibody (OX-7) - BSA Free [NB100-65543]

Flow Cytometry: CD90/Thy1 Antibody (OX-7) - BSA Free [NB100-65543]

Flow Cytometry: CD90/Thy1 Antibody (OX-7) [NB100-65543] - Staining of rat thymus.
Flow Cytometry: CD90/Thy1 Antibody (OX-7) - BSA Free [NB100-65543]

Flow Cytometry: CD90/Thy1 Antibody (OX-7) - BSA Free [NB100-65543]

Flow Cytometry: CD90/Thy1 Antibody (OX-7) [NB100-65543] - Staining of rat thymus with MOUSE ANTI RAT CD90 (THY 1.1).
Flow Cytometry: CD90/Thy1 Antibody (OX-7) - BSA Free [NB100-65543]

Flow Cytometry: CD90/Thy1 Antibody (OX-7) - BSA Free [NB100-65543]

Flow Cytometry: CD90/Thy1 Antibody (OX-7) [NB100-65543] - Staining of rat thymocytes.
Flow Cytometry: CD90/Thy1 Antibody (OX-7) - BSA Free [NB100-65543]

Flow Cytometry: CD90/Thy1 Antibody (OX-7) - BSA Free [NB100-65543]

Flow Cytometry: CD90/Thy1 Antibody (OX-7) [NB100-65543] - Staining of rat thymocytes.
Flow Cytometry: CD90/Thy1 Antibody (OX-7) - BSA Free [NB100-65543]

Flow Cytometry: CD90/Thy1 Antibody (OX-7) - BSA Free [NB100-65543]

Flow Cytometry: CD90/Thy1 Antibody (OX-7) [NB100-65543] - Staining of rat thymus cell.

Applications for CD90/Thy1 Antibody (OX-7) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry Free-Floating

reported in scientific literature (PMID 35383427)

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul.

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD90/Thy1

CD90 (Thy1) antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1.

Alternate Names

CD90, Thy1

Entrez Gene IDs

21838 (Mouse); 24832 (Rat); 100339279 (Rabbit)

Gene Symbol

THY1

UniProt

P01830 (Rat)

Additional CD90/Thy1 Products

Product Documents for CD90/Thy1 Antibody (OX-7) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CD90/Thy1 Antibody (OX-7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD90/Thy1 Antibody (OX-7) - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD90/Thy1 Antibody (OX-7) - BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Can I use Cd90/Thy1antibody (OX-7) NB100-65543 for mouse tissue to do Western Blot? What additional optimization is required?

    A: Our datasheet says that this antibody can be used in these applications: WB, Flow, IHC-Fr, and IP with the samples from mouse, rat, guinea pig, and rabbit. If using this antibody in above applications with the sample(s) from the species, we guarantee it will produce the positive results, or we will refund or replace the antibody free. [When using in IHC-Fr, the mouse-on-mouse (http://vectorlabs.com/mouse-on-mouse-m-o-m-blocking-reagent.html) will be required]. It does not have any special instructions for using this antibody in WB, and therefore our general WB protocol can apply: Protocol. Our recommendation for dilution in WB: 1:100-1:2000. Use the above protocol/dilution to start a small test first. After getting the positive result(s), you could adjust the dilution factor of the antibody as you are repeating/duplicating the experiment, to obtain the best image(s).

  • Q: We are interested in CD90 antibodies that could react with dog, please let me know which products in your catalog you would recommend.

    A: I would recommend one of theses CD90 antibodies, as these have all been validated for use with dog/canine samples.

  • Q: Can I use Cd90/Thy1antibody (OX-7) NB100-65543 for mouse tissue to do Western Blot? What additional optimization is required?

    A: Our datasheet says that this antibody can be used in these applications: WB, Flow, IHC-Fr, and IP with the samples from mouse, rat, guinea pig, and rabbit. If using this antibody in above applications with the sample(s) from the species, we guarantee it will produce the positive results, or we will refund or replace the antibody free. [When using in IHC-Fr, the mouse-on-mouse (http://vectorlabs.com/mouse-on-mouse-m-o-m-blocking-reagent.html) will be required]. It does not have any special instructions for using this antibody in WB, and therefore our general WB protocol can apply: Protocol. Our recommendation for dilution in WB: 1:100-1:2000. Use the above protocol/dilution to start a small test first. After getting the positive result(s), you could adjust the dilution factor of the antibody as you are repeating/duplicating the experiment, to obtain the best image(s).

  • Q: We are interested in CD90 antibodies that could react with dog, please let me know which products in your catalog you would recommend.

    A: I would recommend one of theses CD90 antibodies, as these have all been validated for use with dog/canine samples.

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