CD90/Thy1 Antibody
Novus Biologicals | Catalog # NBP1-51963
![Western Blot: CD90/Thy1 Antibody [NBP1-51963]](https://beta-resources.rndsystems.com/images/products/CD90-Thy1-Antibody-Western-Blot-NBP1-51963-img0001.jpg "Western Blot: CD90/Thy1 Antibody [NBP1-51963]")
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Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Canine (100%), Porcine (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Peptide with sequence C-PEHTYRSRTNFTSKY, from the internal region of the protein sequence according to NP_006279.2.
Marker
Mesenchymal Cell Marker
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Novus Biologicals Goat CD90/Thy1 Antibody (NBP1-51963) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-CD90/Thy1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for CD90/Thy1 Antibody
Western Blot: CD90/Thy1 Antibody [NBP1-51963]
Western Blot: CD90/Thy1 Antibody [NBP1-51963] - (0.1ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.![Immunohistochemistry-Paraffin: CD90/Thy1 Antibody [NBP1-51963]](https://beta-resources.rndsystems.com/images/products/CD90-Thy1-Antibody-Immunohistochemistry-Paraffin-NBP1-51963-img0002.jpg "Immunohistochemistry-Paraffin: CD90/Thy1 Antibody [NBP1-51963]")
Immunohistochemistry-Paraffin: CD90/Thy1 Antibody [NBP1-51963]
Immunohistochemistry-Paraffin: CD90/Thy1 Antibody [NBP1-51963] - (3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for CD90/Thy1 Antibody
Application
Recommended Usage
Immunohistochemistry
3.75 ug/ml
Immunohistochemistry-Paraffin
3.75 ug/ml
Peptide ELISA
detection limit 1:64000
Western Blot
0.1-0.3 ug/ml
Application Notes
This antibody works in ELISA and Western Blot. Approx 26kDa band observed in Human Brain (Cerebellum) and Human Tonsil lysates (calculated MW of 17.9kDa according to NP_006279.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Brain (Cortex).
IHC: Paraffin embedded Human Brain (Cortex).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CD90/Thy1
Additional CD90/Thy1 Products
Product Documents for CD90/Thy1 Antibody
Product Specific Notices for CD90/Thy1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for CD90/Thy1 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for CD90/Thy1 Antibody
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Q: We are interested in CD90 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
A: I would recommend one of theses CD90 antibodies, as these have all been validated for use with dog/canine samples.
