T cell columns have a wide range of loading capacities and are available in several sizes for processing variable numbers of cells.
(A) A CD3-FITC and CD8-PE stained mouse mononuclear cell preparation prior to loading on a mouse CD4+ subset enrichment column. (B) A CD3-FITC and CD8-PE stained sample following application to a mouse CD4+ enrichment selection column. Note that all of the CD3+/CD8+ T cells have been depleted from the mouse mononuclear cell preparation.
(A) Immunophenotyping analysis of human PMNs prior to being loaded onto Eosinophil Enrichment Columns. The frequency of eosinophils, CD16 dull and CD49d positive cells, in the starting population is approximately 3.4% of loaded cells. (B) CD16-FITC and CD49d-PE staining analysis of cells eluted from the Eosinophil Enrichment Columns. The eosinophil purity of the recovered cell population is approximately 98%.
The isolation of eosinophils from human peripheral blood is desirable for the study of many immunologic processes. The columns are intended to provide a highly enriched population of eosinophils with minimal CD16+ neutrophil or lymphocyte contamination.