Human CD45 Phycoerythrin MAb (Clone 2D1)

Catalog # Availability Size / Price Qty
FAB1430P-100
FAB1430P-025
Detection of CD45 in Human Blood Lymphocytes by Flow Cytometry.
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Product Details
Citations (10)
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Reviews (1)

Human CD45 Phycoerythrin MAb (Clone 2D1) Summary

Species Reactivity
Human
Specificity
Detects human CD45. This antibody recognizes all isoforms of human CD45.
Source
Monoclonal Mouse IgG1 Clone # 2D1
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human peripheral blood mononuclear cells
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CD45 antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of CD45 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD45 PE-conjugated Monoclonal Antibody (Catalog # FAB1430P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD45

CD45, previously called LCA (Leukocyte Common Antigen), T200, or Ly5 in mice, is member C of the class 1 (Receptor‑like) Protein Tyrosine Phosphatase family (PTPRC) (1, 2). It is a variably glycosylated 180‑220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1‑3). CD45 has several isoforms, expressed according to cell type, developmental stage and antigenic exposure (1‑5). The longest form, CD45RABC (called B220 in mouse), is expressed on B lymphocytes (5). The CD45RABC cDNA encodes 1304 amino acids (aa), including a 23 aa signal sequence, a 552 aa extracellular domain containing the splicing region, a cysteine‑rich region and two fibronectin type III domains, a 22 aa transmembrane sequence, and a 707 aa cytoplasmic domain that contains two phosphatase domains, D1 and D2. Only D1 has phosphatase activity. CD45R0 is the shortest form, lacking exons 4, 5 and 6 which encode aa 32‑191. It is expressed on memory cells, while intermediate sizes are expressed on other T cells (3, 4, 6). CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds (3, 6‑8). CD45 is moved into or out of the Immunological Synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton (9, 10). Galectin interaction can be fine‑tuned by varying usage of the heavily O‑glycosylated spliced regions and sialylation of N‑linked carbohydrates (4, 9). Within the IS, CD45 dephosphorylates and negatively regulates the Src family kinase, Lck (8‑10). In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP‑1/CD148 (11‑14). CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders (6, 7).

References
  1. Anderson, J.N. et al. (2004) FASEB J. 18:8.
  2. Streuli, M. et al. (1987) J. Exp. Med. 166:1548.
  3. Hermiston, M.L. et al. (2003) Annu. Rev. Immunol. 21:107.
  4. Earl, L.A. and L.G. Baum (2008) Immunol. Cell Biol. 86:608.
  5. Ralph, S.J. et al. (1987) EMBO J. 6:1251.
  6. Falahti, R. and D. Leitenberg (2008) J. Immunol. 181:6082.
  7. Tchilian, E.Z. and P.C.L. Beverley (2006) Trends Immunol. 27:146.
  8. McNiell, L. et al. (2007) Immunity 27:425.
  9. Chen, I-J. et al. (2007) J. Biol. Chem. 282:35361.
  10. Freiberg, B.A. et al. (2002) Nat. Immunol. 3:911.
  11. Zhu, J.W. et al. (2008) Immunity 28:183.
  12. Huntington, N.D. et al. (2006) Nat. Immunol. 7:190.
  13. Hesslein, D.G. et al. (2006) Proc. Natl. Acad. Sci. USA 103:7012.
  14. Cross, J.L. et al. (2008) J. Immunol. 180:8020.
Long Name
Cluster of Differentiation 45
Entrez Gene IDs
5788 (Human); 19264 (Mouse); 490255 (Canine); 100061950 (Equine)
Alternate Names
B220; CD_antigen: CD45; CD45 antigen; CD45; CD45R; EC 3.1.3.48; EC:3.1.3.48; GP180; LCA; L-CA; Leukocyte common antigen; LY5; protein tyrosine phosphatase, receptor type, c polypeptide; PTPRC; receptor-type tyrosine-protein phosphatase C; T200 Glycoprotein; T200 leukocyte common antigen; T200

Product Datasheets

Citations for Human CD45 Phycoerythrin MAb (Clone 2D1)

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. CD44v6 Defines a New Population of Circulating Tumor Cells Not Expressing EpCAM
    Authors: G Belthier, Z Homayed, F Grillet, C Duperray, J Vendrell, I Krol, S Bravo, JC Boyer, O Villeronce, J Vitre-Boub, D Heaug-Wane, F Macari-Fin, J Smith, M Merlot, G Lossaint, T Mazard, F Portales, J Solassol, M Ychou, N Aceto, E Mamessier, F Bertucci, JM Pascussi, E Samalin, F Hollande, J Pannequin
    Cancers, 2021;13(19):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Different concentrations of C5a affect human dental pulp mesenchymal stem cells differentiation
    Authors: J Liu, X Wei, J Hu, X Tan, X Kang, L Gao, N Li, X Shi, M Yuan, W Hu, M Liu
    BMC Oral Health, 2021;21(1):470.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Opposing immune and genetic mechanisms shape oncogenic programs in synovial sarcoma
    Authors: L Jerby-Arno, C Neftel, ME Shore, HR Weisman, ND Mathewson, MJ McBride, B Haas, B Izar, A Volorio, G Boulay, L Cironi, AR Richman, LC Broye, JM Gurski, CC Luo, R Mylvaganam, L Nguyen, S Mei, JC Melms, C Georgescu, O Cohen, JE Buendia-Bu, A Segerstolp, M Sud, MS Cuoco, D Labes, S Gritsch, DR Zollinger, N Ortogero, JM Beechem, G Petur Niel, I Chebib, T Nguyen-Ngo, M Montemurro, GM Cote, E Choy, I Letovanec, S Cherix, N Wagle, PK Sorger, AB Haynes, JT Mullen, I Stamenkovi, MN Rivera, C Kadoch, KW Wucherpfen, O Rozenblatt, ML Suvà, N Riggi, A Regev
    Nature Medicine, 2021;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Membranes derived from human umbilical cord Wharton's jelly stem cells as novel bioengineered tissue-like constructs
    Authors: BD Jaimes-Par, I Garzón, V Carriel, D Durand-Her, MA Martín-Pie, JM García, MC Sánchez-Qu, M Alaminos, A Campos
    Histol. Histopathol., 2017;0(0):11897.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Innovative Flow Cytometry Allows Accurate Identification of Rare Circulating Cells Involved in Endothelial Dysfunction
    PLoS ONE, 2016;11(8):e0160153.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Amniotic Fluid Derived Stem Cells with a Renal Progenitor Phenotype Inhibit Interstitial Fibrosis in Renal Ischemia and Reperfusion Injury in Rats.
    Authors: Monteiro Carvalho Mori da M, Zia S, Oliveira Arcolino F, Carlon M, Beckmann D, Pippi N, Luhers Graca D, Levtchenko E, Deprest J, Toelen J
    PLoS ONE, 2015;10(8):e0136145.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Human fetal and adult bone marrow-derived mesenchymal stem cells use different signaling pathways for the initiation of chondrogenesis.
    Authors: Brady K, Dickinson S, Guillot P, Polak J, Blom A, Kafienah W, Hollander A
    Stem Cells Dev, 2014;23(5):541-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Signaling hierarchy regulating human endothelial cell development.
    Authors: Kelly MA, Hirschi KK
    Arterioscler. Thromb. Vasc. Biol., 2009;29(5):718-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. CCR4-expressing T cell tumors can be specifically controlled via delivery of toxins to chemokine receptors.
    Authors: Baatar D, Olkhanud P, Newton D, Sumitomo K, Biragyn A
    J. Immunol., 2007;179(3):1996-2004.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Wharton's jelly stem cells: a novel cell source for oral mucosa and skin epithelia regeneration.
    Authors: Garzon I, Miyake J, Gonzalez-Andrades M, Carmona R, Carda C, Sanchez-Quevedo M, Campos A, Alaminos M
    Stem Cells Transl Med, 0;2(8):625-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human CD45 PE-conjugated Antibody
By Anonymous on 08/16/2016
Application: Flow Sample Tested: Human embryonic stem cells (H9 from Wicell) Species: Human