Claudin-1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-77036

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Canine

Cited:

Human, Mouse, Rat, Canine

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Claudin-1 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 20 amino acids near the carboxy terminus of human Claudin-1. The immunogen is located within the last 50 amino acids of Claudin-1. Amino Acid Sequence: PRKTTSYPTPRPYPKPAPS

Reactivity Notes

Porcine reactivity per customer review. Mouse reactivity reported in (PMID: 33392105), Canine reactivity reported in (PMID: 32419902).

Specificity

Human Claudin-1 has one isoform (211aa, 23kD). Mouse Claudin-1 has one isoform (211aa, 23kD) and Rat Claudin-1 also has one isoform (211aa, 23kD). NBP1-77036 can detect human, mouse and rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Claudin-1 Antibody - BSA Free

Western Blot: Claudin-1 AntibodyBSA Free [NBP1-77036]

Western Blot: Claudin-1 AntibodyBSA Free [NBP1-77036]

Claudin-1-Antibody-Western-Blot-NBP1-77036-img0012.jpg
Western Blot: Claudin-1 AntibodyBSA Free [NBP1-77036]

Western Blot: Claudin-1 AntibodyBSA Free [NBP1-77036]

Western Blot: Claudin-1 Antibody - BSA Free [NBP1-77036] - Claudin-1 Antibody [NBP1-77036] - Validation in Human Tissue Lysates Loading: 15 ug of lysates per lane.Antibodies: CLDN1, (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: Claudin-1 AntibodyBSA Free [NBP1-77036]

Western Blot: Claudin-1 AntibodyBSA Free [NBP1-77036]

Western Blot: Claudin-1 Antibody - BSA Free [NBP1-77036] - Claudin-1 Antibody [NBP1-77036] - Validation in Human HepG2 Cell Lysate Loading: 15 ug of lysates per lane.Antibodies: CLDN1, 5187 (A: 1 gu/mL and B: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: Claudin-1 AntibodyBSA Free [NBP1-77036]

Western Blot: Claudin-1 AntibodyBSA Free [NBP1-77036]

Western Blot: Claudin-1 Antibody - BSA Free [NBP1-77036] - Claudin-1 Antibody [NBP1-77036] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: CLDN1, NBP1-77036 (1 ug/mL), CLDN1, (2 ug/mL), and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: Claudin-1 AntibodyBSA Free [NBP1-77036]

Western Blot: Claudin-1 AntibodyBSA Free [NBP1-77036]

Western Blot: Claudin-1 Antibody - BSA Free [NBP1-77036] - Claudin-1 Antibody [NBP1-77036] - Validation in Human Tissue Lysates Loading: 15 ug of lysates per lane.Antibodies: CLDN1,(1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunocytochemistry/ Immunofluorescence: Claudin-1 Antibody - BSA Free [NBP1-77036]

Immunocytochemistry/ Immunofluorescence: Claudin-1 Antibody - BSA Free [NBP1-77036]

Immunocytochemistry/Immunofluorescence: Claudin-1 Antibody - BSA Free [NBP1-77036] - Claudin-1 Antibody [NBP1-77036] - Claudin-1 labeled pig cultured cornea cells visualized with Alexa488. This image was submitted via customer Review.
Immunocytochemistry/ Immunofluorescence: Claudin-1 Antibody - BSA Free [NBP1-77036]

Immunocytochemistry/ Immunofluorescence: Claudin-1 Antibody - BSA Free [NBP1-77036]

Immunocytochemistry/Immunofluorescence: Claudin-1 Antibody - BSA Free [NBP1-77036] - Claudin-1 Antibody [NBP1-77036] - Claudin-1 labeled pig Sc cells visualized with Alexa488. This image was submitted via customer Review.
Claudin-1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Claudin-1 Antibody - BSA Free [NBP1-77036] -

Immunocytochemistry/ Immunofluorescence: Claudin-1 Antibody - BSA Free [NBP1-77036] - Validation of Claudin-1 in HepG2 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Claudin-1 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Applications for Claudin-1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

5-20 ug/mL

Western Blot

1-2 ug/ml
Application Notes
Use in ICC/IF reported in multiple pieces of scientific literature. Use in IHC reported in scientific literature (PMID:32067099).

Reviewed Applications

Read 2 reviews rated 3.5 using NBP1-77036 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Claudin-1

Claudin1 (CLDN1), a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Tight junctions are specialized regions of cell to cell contact consisting of networking strands that act as a molecular gasket for preventing the leakage of ions, water, etc., between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition and CLDN1 expression is often cell type and tissue dependent. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. CLDN1 and CLDN2 were found to be overexpressed in colonal cancer tissues and may be useful as tumor markers and targets for the treatment of colorectal cancer. Characterization of Claudins expression in human tumors can be an additional diagnostic tool. Recent studies show that CLDN1 has gastric tumor suppressive activity and is a direct transcriptional target of RUNX3. Along with SCARB1, LDL-R, and the tetraspanin superfamily member CD81, CLDN1 has been reported to be an entry factor for the Hepatitis C virus.

Alternate Names

Claudin1, CLD1, CLDN1, SEMP1

Entrez Gene IDs

9076 (Human); 12737 (Mouse); 65129 (Rat)

Gene Symbol

CLDN1

UniProt

O95832

Additional Claudin-1 Products

Product Documents for Claudin-1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Claudin-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Claudin-1 Antibody - BSA Free

Customer Reviews for Claudin-1 Antibody - BSA Free (2)

3.5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
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3 Stars
50%
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1 Stars
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Claudin-1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: pig Sc cells
    Species: Pig
    Verified Customer | Posted 06/07/2017
    Claudin-1 Antibody (NBP1-77036) labeling pig Sc cells with Alexa488
    Claudin-1 Antibody - BSA Free NBP1-77036
  • Claudin-1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: pig cultured cornea cells
    Species: Pig
    Verified Customer | Posted 06/07/2017
    Claudin-1 Antibody (NBP1-77036) labeling pig cultured cornea cells with Alexa488
    Claudin-1 Antibody - BSA Free NBP1-77036

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