Collagen I alpha 1 Antibody (COL-1)

Novus Biologicals | Catalog # NB600-450

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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Equine, Feline, Mammal, Rabbit

Cited:

Human, Mouse, Rat, Equine, Feline, Rabbit

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Dot Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, IF/IHC, Electron Microscopy

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # COL-1
View all Collagen I alpha 1 Primary Antibodies »

Product Specifications

Immunogen

This Collagen I alpha 1 antibody (COL-1) was raised against full length bovine native protein (purified).

Epitope

The epitope recognized by the antibody may be sensitive to routine formalin fixation and paraffin embedding.

Reactivity Notes

Reported reactivity in deer. Mouse reactivity reported in scientific literature (PMID: 25287675). Goat reactivity reported by customer review. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions. Feline reactivity reported in scientific literature (PMID:33091431).

Localization

Extracellular matrix

Specificity

This Collagen I alpha 1 antibody (COL-1) recognizes the native (helical) form of collagen type I using ELISA and dot-blot. It does not react with the thermally-denatured molecule, and shows no cross-reactivity with collagen types II, III, IV, V, VI, VII, IX, X and XI.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

139 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Collagen I alpha 1 Antibody (COL-1)

Immunohistochemistry-Frozen: Collagen I alpha 1 Antibody (COL-1) [NB600-450]

Immunohistochemistry-Frozen: Collagen I alpha 1 Antibody (COL-1) [NB600-450]

Immunohistochemistry-Frozen: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Staining of acetone-fixed, frozen sections of pig tongue at a dilution of 1:4000
Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450]

Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450]

Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Analysis of an FFPE ligament section. Enzymatic antigen retrieval and blocking using 1% BSA for 30 min at 20C was performed. Sample was incubated for 1 hour with primary antibody at a dilution of 1:400 at 20C.
Immunocytochemistry/ Immunofluorescence: Collagen I alpha 1 Antibody (COL-1) [NB600-450]

Immunocytochemistry/ Immunofluorescence: Collagen I alpha 1 Antibody (COL-1) [NB600-450]

Collagen-I-alpha-1-Antibody-COL-1-Immunocytochemistry-Immunofluorescence-NB600-450-img0014.jpg
Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450]

Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450]

Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Staining of acetone-fixed, frozen sections of human tongue at a dilution of 1:2000.
Immunocytochemistry/ Immunofluorescence: Collagen I alpha 1 Antibody (COL-1) [NB600-450]

Immunocytochemistry/ Immunofluorescence: Collagen I alpha 1 Antibody (COL-1) [NB600-450]

Immunocytochemistry/Immunofluorescence: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Analysis of Collagen I alpha 1 in 4% PFA fixed equine tenocytes using anti-Collagen I alpha 1 antibody. ICC/IF image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: Collagen I alpha 1 Antibody (COL-1) [NB600-450]

Immunocytochemistry/ Immunofluorescence: Collagen I alpha 1 Antibody (COL-1) [NB600-450]

Immunocytochemistry/Immunofluorescence: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Collagen I alpha1 labeled pig trabecular meshwork cells indirectly visualized with Alexa Fluor 488 conjugated secondary antibody. ICC/IF image submitted by a verified customer review.
Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450]

Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450]

Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Analysis of formalin fixed frozen porcine skin tissue sections. Blocking: 5% serum for 1 hr at 20C. Sample was incubated for 16 hours at 4C and used at a dilution of 1:500.
Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450]

Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450]

Collagen-I-alpha-1-Antibody-COL-1-Immunohistochemistry-NB600-450-img0013.jpg
Immunohistochemistry-Paraffin: Collagen I alpha 1 Antibody (COL-1) [NB600-450]

Immunohistochemistry-Paraffin: Collagen I alpha 1 Antibody (COL-1) [NB600-450]

Immunohistochemistry-Paraffin: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Mouse healthy liver. Anibody at 1:200. Enzymatic/proteolytic antigen retrieval. No permeabilization. IHC-P image submitted by a verified customer review.
Collagen I alpha 1 Antibody (COL-1)

Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] -

(a) Histological section of keloid stained with picrosirius seen in ordinary light (20x). (b) Histological section of keloid stained with picrosirius seen in polarized light (20x). (c) Immunohistochemistry for type I collagen in keloid (20x). (d) Histological section of keloid stained with picrosirius seen in ordinary light (20x). (e) Histological section of keloid stained with picrosirius seen in polarized light (20x). (f) Immunohistochemistry for type III collagen in keloid (20x).
Collagen I alpha 1 Antibody (COL-1)

Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] -

Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - FSP‐1–positive cells only partially responsible for collagen I & III production in irradiated bladders. Bladder strips from irradiated mice were costained for collagen I or for collagen III mRNA (pink) & for fibroblasts (FSP‐1, brown). U: urothelium; LP: lamina propria. Black arrow: FSP‐1 positive cells. Gray arrow: collagen only positive cells. Scale bar = 50 µm Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32109348), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Collagen I alpha 1 Antibody (COL-1)

Immunocytochemistry/ Immunofluorescence: Collagen I alpha 1 Antibody (COL-1) [NB600-450] -

Collagen I and collagen III accumulate in C57BL/6 mice in response to radiation. Bladder strips from irradiated and control mice were stained for collagen I or for collagen III and imaged using fluorescent microscopy. (a) Representative images (100 x 100 um) of each mouse strain and treatment group are provided for collagen I and collagen III immunofluorescence. (b–c) Percentage of tissue‐positive for collagen I or collagen III staining. Collagen I and III density is significantly elevated in C57BL/6 mice, but not in the C3H or BALB/c strains. Results are mean +/- SD of n = 3–6 mice. Dashed line: ANOVA; Full line: multiple t‐test. **p <.01, *** p <.001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32109348), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Collagen I alpha 1 Antibody (COL-1)

Application
Recommended Usage

Dot Blot

1:100-1:2000

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:2000

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:100-1:2000

Reviewed Applications

Read 5 reviews rated 4.6 using NB600-450 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

15mM Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Collagen I alpha 1

Collagen is an extracellular matrix protein that serves as a scaffold defining the shape and mechanical properties of many tissues and organs including skin, tendon, artery walls, fibrocartilage, bone and teeth. Collagens are highly conserved and are characterized by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. The extensive family of collagens is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagen type I (also known as collagen alpha, COL1A1, and alpha-1 type I collagen) is the largest component of fibrillar collagen found in cartilage and connective tissues. It is synthesized by fibroblasts, osteoblasts, and odontoblasts and has a theoretical molecular weight of 138 kDa.

Type I collagen is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon tissue. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue. Several collagens play a role in cell adhesion, responsible for maintaining normal tissue architecture and function. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Post-Golgi LH3 trafficking is essential for collagen homeostasis and for the development and function of multiple organs and tissues (1).

The COL1A1 gene encodes the pro-alpha1 chains of type I collagen protein, whose triple helix is comprised of two alpha1 chains and one alpha2 chain. Mutations in the encoding COL1A1 gene are associated with brittle bone disease (Osteogenesis Imperfecta), cortical hyperostosis (Caffey disease) and disorders that affect the connective tissues (Ehlers-Danlos syndrome) (2). Studies have found that HIF-1 transcription regulation of collagen prolyl hydroxylases regulates collagen deposition, promoting cancer cell alignment along collagen fibers, which enhances invasion and metastasis to lymph nodes and lung tissue by breast cancer cells (3).

References

1. Banushi, B., Forneris, F., Straatman-Iwanowska, A., Strange, A., Lyne, A. M., Rogerson, C.,... Gissen, P. (2016). Regulation of post-Golgi LH3 trafficking is essential for collagen homeostasis. Nat Commun, 7, 12111. doi:10.1038/ncomms12111

2. Lu, Y., Zhang, S., Wang, Y., Ren, X., & Han, J. (2019). Molecular mechanisms and clinical manifestations of rare genetic disorders associated with type I collagen. Intractable Rare Dis Res, 8(2), 98-107. doi:10.5582/irdr.2019.01064

3. Gilkes, D. M., Chaturvedi, P., Bajpai, S., Wong, C. C., Wei, H., Pitcairn, S.,... Semenza, G. L. (2013). Collagen prolyl hydroxylases are essential for breast cancer metastasis. Cancer Res, 73(11), 3285-3296. doi:10.1158/0008-5472.Can-12-3963

Alternate Names

COL1A1, OI4

Entrez Gene IDs

1277 (Human); 29393 (Rat)

Gene Symbol

COL1A1

Additional Collagen I alpha 1 Products

Product Documents for Collagen I alpha 1 Antibody (COL-1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Collagen I alpha 1 Antibody (COL-1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Collagen I alpha 1 Antibody (COL-1)

Customer Reviews for Collagen I alpha 1 Antibody (COL-1) (5)

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Customer Images

Showing  1 - 5 of 5 reviews Showing All
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  • Collagen I alpha 1 Antibody (COL-1)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Liver
    Species: Mouse
    Verified Customer | Posted 06/24/2021
    Mouse healthy liver
    Primary antibody dilution 1:200 Enzymatic/proteolytic antigen retrieval No Permeabilization
    Collagen I alpha 1 Antibody (COL-1) NB600-450
  • Collagen I alpha 1 Antibody (COL-1)
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Chondrocyte
    Species: Goat
    Verified Customer | Posted 01/22/2019
    Collagen I alpha 1 Antibody (COL-1) NB600-450
  • Collagen I alpha 1 Antibody (COL-1)
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Sclera
    Species: Feline
    Verified Customer | Posted 09/15/2018
    NB600-450 was secondary immunolabelied shown as red. DAPI (blue). The image was captured using epifluorescent microscope.
    Collagen I alpha 1 Antibody (COL-1) NB600-450
  • Collagen I alpha 1 Antibody (COL-1)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Pig trabecular meshwork cells
    Species: Pig
    Verified Customer | Posted 06/07/2017
    NB600-450 Collagen I alpha1 antibody labeling pig trabecular meshwork cells with Alexa488
    Collagen I alpha 1 Antibody (COL-1) NB600-450
  • Name: Jessica Ochoa
    Application: Immunofluorescence
    Sample Tested: Equine Tenocytes
    Species: Other
    Verified Customer | Posted 04/21/2015
    Collagen I-positive equine tenocytes
    Collagen I alpha 1 Antibody (COL-1) NB600-450

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Protocols

View specific protocols for Collagen I alpha 1 Antibody (COL-1) (NB600-450):


Sample
Human Tissue sections (Normal Skin and Basal Cell Carcinoma/Solar Elastos)

Application
Immunohistochemistry (Formalin-fixed paraffin-embedded sections)

Blocking step:
None

Antigen retrieval step:
Heat mediated

Incubation time:
30 minute(s)

Dilution: 1/800

Additional notes
The tissue was FFPE (formalin fixed, paraffin embedded tissue sectioned at 3-4 microns). The antigen retreival method was a heat-induced antigen retreival (HEIR) using a vegetable steamer. Sections were incubated in the steamer for 20 minutes in 1 mM EDTA buffer, pH 8.0. Sections were then rinsed in H2O and TBS buffer prior to starting the staining protocol.

The staining appeared to be very specific and robust, with very little to no non-specific background staining.

Staining Summary & Notes:
1mM EDTA Buffer, pH 8.0, HIER for 20 minutes in steamer
Rinse with TBS containing 0.05% Tween 20
10 minute blocking in 3% Peroxide
Rinse with TBS containing 0.05% Tween 20
30 minutes in Primary Antibody @ RT
Rinse with TBS containing 0.05% Tween 20
30 minutes in Secondary Antibody @ RT
Rinse with TBS containing 0.05% Tween 20
10 minutes in DAB+ (DakoCytomation) @ RT
Water Rinse
Counterstain for 6 minutes in Mayer's Hematoxylin (DakoCytomation) @ RT

Secondary antibody
Name:
DakoCytomation Envision+ Anti-Mouse/HRP (RTU)
Conjugation: HRP
Dilution: 1/1

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Collagen I alpha 1 Antibody (COL-1)

Showing  1 - 5 of 5 FAQs Showing All

  • Q: How long does delivery take for your collagen 1 alpha 1 antibody?

    A: Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.

  • Q: I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?

    A: Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).

  • Q: The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?

    A: Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.

  • Q: We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?

    A:

    We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).

  • Q: What is the dilution factor for IHC for starting? Because the range of dilution factor on the datasheet is so broad, I am confused which dilution factor is suitable for starting.

    A: I can recommend the following dilution to start. Keep in mind that these will all vary greatly by sample type, so these are just suggestions. You may need to use more or less antibody. For catalog number NB600-450, the dilution range is 1:1000-1:2000.

  • Q: How long does delivery take for your collagen 1 alpha 1 antibody?

    A: Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.

  • Q: I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?

    A: Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).

  • Q: The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?

    A: Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.

  • Q: We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?

    A:

    We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).

  • Q: What is the dilution factor for IHC for starting? Because the range of dilution factor on the datasheet is so broad, I am confused which dilution factor is suitable for starting.

    A: I can recommend the following dilution to start. Keep in mind that these will all vary greatly by sample type, so these are just suggestions. You may need to use more or less antibody. For catalog number NB600-450, the dilution range is 1:1000-1:2000.

  • Q: How long does delivery take for your collagen 1 alpha 1 antibody?

    A: Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.

  • Q: I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?

    A: Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).

  • Q: The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?

    A: Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.

  • Q: We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?

    A:

    We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).

  • Q: What is the dilution factor for IHC for starting? Because the range of dilution factor on the datasheet is so broad, I am confused which dilution factor is suitable for starting.

    A: I can recommend the following dilution to start. Keep in mind that these will all vary greatly by sample type, so these are just suggestions. You may need to use more or less antibody. For catalog number NB600-450, the dilution range is 1:1000-1:2000.

  • Q: How long does delivery take for your collagen 1 alpha 1 antibody?

    A: Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.

  • Q: I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?

    A: Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).

  • Q: The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?

    A: Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.

  • Q: We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?

    A:

    We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).

  • Q: What is the dilution factor for IHC for starting? Because the range of dilution factor on the datasheet is so broad, I am confused which dilution factor is suitable for starting.

    A: I can recommend the following dilution to start. Keep in mind that these will all vary greatly by sample type, so these are just suggestions. You may need to use more or less antibody. For catalog number NB600-450, the dilution range is 1:1000-1:2000.

  • Q: How long does delivery take for your collagen 1 alpha 1 antibody?

    A: Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.

  • Q: I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?

    A: Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).

  • Q: The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?

    A: Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.

  • Q: We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?

    A:

    We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).

  • Q: What is the dilution factor for IHC for starting? Because the range of dilution factor on the datasheet is so broad, I am confused which dilution factor is suitable for starting.

    A: I can recommend the following dilution to start. Keep in mind that these will all vary greatly by sample type, so these are just suggestions. You may need to use more or less antibody. For catalog number NB600-450, the dilution range is 1:1000-1:2000.

Showing  1 - 5 of 5 FAQs Showing All
View all FAQs for Antibodies