Conditioned Media for Embryonic & Induced Pluripotent Stem Cell Culture

Media for Cell Culture

Culture media specifically optimized for use with human and rodent stem and progenitor cells.

Researchers employing stem or progenitor cells in their studies currently use a wide variety of media products to support their cultures. These media are generally developed or validated for cells other than stem cells, thus making them less desirable than cell type-specific media. To address this need, R&D Systems offers a media product specifically designed for stem/progenitor cell culture. All the components included within this defined, basal, serum-free formulation have been selected, optimized, and verified for embryonic stem cell culture.

Mouse Embryonic Fibroblast Conditioned Media (Catalog # AR005)

Serum-free media (80% Knockout™ D-MEM, 20% Knockout serum replacement, 1% MEM non-essential amino acids, 2 mM GlutaMAX™, 0.1 mM beta-mercaptoethanol, 4 ng/mL basic fibroblast growth factor (FGF basic)) was conditioned by gamma-irradiated CF-1 fibroblasts at 37° C for 24 hours.

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SSEA-4 Expression in Embryonic Stem Cells. Human embryonic stem cells were cultured with recombinant human FGF basic (Catalog # 233-FB) in the presence (A) or absence (B) of Mouse Embryonic Fibroblast (MEF)-conditioned media (Catalog # AR005). SSEA-4 and Oct-3/4 were detected using anti-human SSEA-4 monoclonal antibody (red, Catalog # MAB1435) and anti-human Oct-3/4 polyclonal antibody (green, Catalog # AF1759). Cells were counterstained with DAPI (blue). Image courtesy of Dr. Frank Soldner of the National Institutes of Health.

Knockout, GlutaMAX, and KSR are trademarks of Life Technologies, Carlsbad, CA.
GlobalStem ES-DMEM is a trademark of GlobalStem, Rockville, MD.