Human Oct-4A Antibody

Catalog # Availability Size / Price Qty
Detection of Human Oct‑4A by Western Blot.
3 Images
Product Details
Citations (3)
Supplemental Products
Reviews (2)

Human Oct-4A Antibody Summary

Species Reactivity
Detects human Oct-4A in Western blots.
Monoclonal Mouse IgG2A Clone # 653108
Protein A or G purified from hybridoma culture supernatant
E.coli-derived recombinant human Oct-4A
Accession # NP_002692
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Oct-4A antibody by Western Blot. View Larger

Detection of Human Oct‑4A by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and NTera-2 human testicular embryonic carcinoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human Oct-4A Monoclonal Antibody (Catalog # MAB17591) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Oct-4A at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Oct-4A and E-Cadherin antibody in BG01V Human Stem Cells by Immunocytochemistry (ICC). View Larger

Oct-4A and E-Cadherin in BG01V Human Stem Cells. Oct-4A and E-Cadherin were detected in human BG01V embryonic stem cells grown on irradiated MEF cells using 10 µg/mL Human Oct-4A Monoclonal Antibody (Catalog # MAB17591) and 10 µg/mL Human E-Cadherin Affinity-purified Polyclonal Antibody (Catalog # AF648). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for Oct-4A using the Northern-Lights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009), and stained for E-Cadherin using the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001). Specific staining of Oct-4A was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry Detection of Oct-4A antibody in BG01V Human Stem Cells antibody by Flow Cytometry. View Larger

Detection of Oct‑4A in BG01V Human Stem Cells by Flow Cytometry. BG01V human embryonic stem cells was stained with Human Oct-4A Monoclonal Antibody (Catalog # MAB17591, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Oct-4A

Oct-3/4, alternately Oct-3 or Oct-4, is POU5F1 (POU domain containing, class 5, transcription factor 1), a 360 amino acid (aa) transcription factor that is expressed in totipotent embryonic stem and germ cells. The human Oct-4, Oct-3/4 or POU5F1 gene can be transcribed into at least three transcripts (Oct-4A, Oct-4B, and Oct-4B1) and generates four protein isoforms by alternative splicing and alternative translation initiation. Oct-4A expression is restricted to embryonic stem (ES) and embryonic carcinoma (EC) cells and is believed to be the transcription factor responsible for the pluripotency properties of embryonic stem (ES) cells. In contrast, Oct-4B/4B1 can be detected in various nonpluripotent cell types and cannot sustain ES cell pluripotency and self-renewal.

Long Name
Octamer-binding Transcription Factor 4A
Entrez Gene IDs
5460 (Human); 18999 (Mouse)
Alternate Names
OCT3;Oct-3;OCT4;Oct-4;OCT4;Oct3/4;OTF3;OTF-3;OTF4;POU domain protein;POU domain, class 5, transcription factor 1;POU5F1; Oct4A; Oct-4A

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Citations for Human Oct-4A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Interactions between ALS-linked FUS and nucleoporins are associated with defects in the nucleocytoplasmic transport pathway
    Authors: YC Lin, MS Kumar, N Ramesh, EN Anderson, AT Nguyen, B Kim, S Cheung, JA McDonough, WC Skarnes, R Lopez-Gonz, JE Landers, NL Fawzi, IRA Mackenzie, EB Lee, JA Nickerson, D Grunwald, UB Pandey, DA Bosco
    Nature Neuroscience, 2021;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Human mid-trimester amniotic fluid (stem) cells lack expression of the pluripotency marker OCT4A
    Authors: F Vlahova, KE Hawkins, AM Ranzoni, KL Hau, R Sagar, P Coppi, AL David, J Adjaye, PV Guillot
    Sci Rep, 2019;9(1):8126.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Genome-Wide Transcriptome and Binding Sites Analyses Identify Early FOX Expressions for Enhancing Cardiomyogenesis Efficiency of hESC Cultures
    Sci Rep, 2016;6(0):31068.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Oct-4A Antibody

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Human Oct-4A Antibody
By Anonymous on 03/12/2022
Application: WB Sample Tested: NTera-2 human testicular embryonic carcinoma cell line Species: Human

Human Oct-4A Antibody
By Anonymous on 01/31/2019
Application: ICC/IF Sample Tested: WS-1 human fetal skin fibroblast cell line Species: Human