Human/Mouse E-Cadherin Antibody

(12 citations)
(4 Reviews)
  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human E-Cadherin in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human E-Cadherin
    Asp155-Ile707
    Accession # P12830
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Simple Western
    5 µg/mL
    See below
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse
E‑Cadherin by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, P19 mouse embryonal carcinoma cell line, and 4T1 mouse breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for E‑Cadherin at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of E‑Cadherin in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Goat Anti-Human/Mouse
E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.
Immunocytochemistry
E‑Cadherin in A549 Human Cell Line. E‑Cadherin was detected in immersion fixed A549 human lung carcinoma cell line untreated (top panel) or treated with 10 ng/mL TGF-beta (Catalog # 240-B, lower panel) for 48 hours using Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
E‑Cadherin in Human Epidermoid Carcinoma Cells. E‑Cadherin was detected in immersion fixed human epidermoid carcinoma cells using 10 µg/mL Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
E‑Cadherin and SOX2 in BG01V Human Stem Cells. E‑Cadherin and SOX2 were detected in BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) and 10 µg/mL Human/Mouse SOX2 Monoclonal Antibody (Catalog # MAB2018). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for E‑Cadherin using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL001) and for SOX2 using the NorthernLights 493-conjugated Anti-Mouse Secondary Antibody (red; Catalog # NL009). Cells were counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human and Mouse E‑Cadherin by Simple WesternTM. Simple Western lane view shows lysates of 4T1 mouse breast cancer cell line, P19 mouse embryonal carcinoma cell line, A431 human epithelial carcinoma cell line, and MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for E‑Cadherin at approximately 128 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: E-Cadherin

Epithelial (E)‑Cadherin (ECAD), also known as cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the Cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E‑Cadherin may also play a role in tumor development, as loss of E‑Cadherin has been associated with tumor invasiveness. E‑Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. E‑Cadherin contains five extracellular calcium‑binding domains of approximately 110 amino acids each.

  • References:
    1. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
    2. Overduin, M. et al. (1995) Science 267:386.
    3. Takeichi, M. (1991) Science 251:1451.
  • Entrez Gene IDs:
    999 (Human); 12550 (Mouse); 83502 (Rat)
  • Alternate Names:
    Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Wnt/?-catenin promotes gastric fundus specification in mice and humans
    Authors: KW McCracken, E Aihara, B Martin, CM Crawford, T Broda, J Treguier, X Zhang, JM Shannon, MH Montrose, JM Wells
    Nature, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  2. Novel role for IL-22 in protection during chronic Mycobacterium tuberculosis HN878 infection
    Authors: P Treerat, O Prince, A Cruz-Lagun, M Muñoz-Torr, MA Salazar-Le, M Selman, B Fallert-Ju, TA Reinhardt, JF Alcorn, D Kaushal, J Zuñiga, J Rangel-Mor, JK Kolls, SA Khader
    Mucosal Immunol, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  3. miR-151a induces partial EMT by regulating E-cadherin in NSCLC cells
    Authors: I Daugaard, KJ Sanders, A Idica, K Vittayaruk, M Hamdorf, JD Krog, R Chow, D Jury, LL Hansen, H Hager, P Lamy, CL Choi, D Agalliu, DG Zisoulis, IM Pedersen
    Oncogenesis, 2017;6(7):e366.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  4. Surface Topography Guides Morphology and Spatial Patterning of Induced Pluripotent Stem Cell Colonies
    Authors: G Abagnale, A Sechi, M Steger, Q Zhou, CC Kuo, G Aydin, C Schalla, G Müller-New, M Zenke, IG Costa, P van Rijn, A Gillner, W Wagner
    Stem Cell Reports, 2017;9(2):654-666.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  5. Canine spontaneous head and neck squamous cell carcinomas represent their human counterparts at the molecular level.
    Authors: Liu D, Xiong H, Ellis A, Northrup N, Dobbin K, Shin D, Zhao S
    PLoS Genet, 2015;11(6):e1005277.
    Species: Canine
    Sample Type: Whole Cells
    Application: IHC Paraffin-embedded
  6. Molecular homology and difference between spontaneous canine mammary cancer and human breast cancer.
    Authors: Liu D, Xiong H, Ellis A, Northrup N, Rodriguez C, O'Regan R, Dalton S, Zhao S
    Cancer Res, 2014;74(18):5045-56.
    Species: Canine
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  7. Penton-dodecahedral particles trigger opening of intercellular junctions and facilitate viral spread during adenovirus serotype 3 infection of epithelial cells.
    Authors: Lu Z, Wang H, Zhang Y, Cao H, Li Z, Fender P, Lieber A
    PLoS Pathog, 2013;9(10):e1003718.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  8. Interleukin-15 plays a central role in human kidney physiology and cancer through the gamma c signaling pathway.
    Authors: Giron-Michel J, Azzi S, Khawam K, Mortier E, Caignard A, Devocelle A, Ferrini S, Croce M, Francois H, Lecru L, Charpentier B, Chouaib S, Azzarone B, Eid P
    PLoS ONE, 2012;7(2):e31624.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  9. Snail involves in the transforming growth factor beta1-mediated epithelial-mesenchymal transition of retinal pigment epithelial cells.
    Authors: Li H, Wang H, Wang F, Gu Q, Xu X
    PLoS ONE, 2011;6(8):e23322.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  10. Tissue engineering of oral dysplasia.
    Authors: Gaballah K, Costea DE, Hills A, Gollin SM, Harrison P, Partridge M
    J. Pathol., 2008;215(3):280-9.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  11. Downregulation of Rap1GAP contributes to Ras transformation.
    Authors: Tsygankova OM, Prendergast GV, Puttaswamy K, Wang Y, Feldman MD, Wang H, Brose MS, Meinkoth JL
    Mol. Cell. Biol., 2007;27(19):6647-58.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  12. Requirement for intercellular adhesion molecule 1 and caveolae in invasion of human oral epithelial cells by Porphyromonas gingivalis.
    Authors: Tamai R, Asai Y, Ogawa T
    Infect. Immun., 2005;73(10):6290-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
Expand to show all 12 Citations
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Average Rating: 4.5 (Based on 4 reviews)

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We have 4 reviews tested in 2 species: Human, Mouse.
We have 4 review tested in 1 application: Immunohistochemistry.

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Images Ratings Applications Species Reviewed By Date Details
Immunohistochemistry Human/Mouse E-Cadherin Antibody AF648
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Very Good
 IHC Human Anonymous 12/10/2017
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Image Details
Immunohistochemistry Human/Mouse E-Cadherin Antibody AF648
Immunohistochemistry: Human/Mouse E-Cadherin Antibody [AF648]

Summary

ApplicationImmunohistochemistry
Sample TestedKidney cancer tissue
SpeciesHuman
Immunohistochemistry Human/Mouse E-Cadherin Antibody AF648
Enlarge
Excellent
 IHC Human Anonymous 08/29/2016
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Image Details
Immunohistochemistry Human/Mouse E-Cadherin Antibody AF648
Immunohistochemistry: Human/Mouse E-Cadherin Antibody [AF648]

Summary

ApplicationImmunohistochemistry
Sample Testedskin tumor
SpeciesHuman

Other Experimental Details

Other Experimental DetailsAntigen retrieval: 10mM Citrate pH6Antibody dilution: 1:400Secondary antibody: Alexa 647 donkey anti-goat secondary antibody
Immunohistochemistry Human/Mouse E-Cadherin Antibody AF648
Enlarge
Excellent
 IHC Mouse Anonymous 08/22/2016
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Image Details
Immunohistochemistry Human/Mouse E-Cadherin Antibody AF648
Immunohistochemistry: Human/Mouse E-Cadherin Antibody [AF648]

Summary

ApplicationImmunohistochemistry
Sample TestedStomach tissue
SpeciesMouse

Other Experimental Details

Other Experimental DetailsAntigen retrieval: 10mM Citrate buffer pH6.Antibody dilution: 1:400Secondary antibody: CY3 conjugated donkey anti-goat secondary antibody @ 1:300
  Very Good
 IHC Human Anonymous 07/19/2016
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Summary

ApplicationImmunohistochemistry
Sample TestedHindgut
SpeciesHuman

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