Human/Mouse E-Cadherin Antibody
Human/Mouse E-Cadherin Antibody Summary
Asp155-Ile707
Accession # P12830
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse E‑Cadherin by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, P19 mouse embryonal carcinoma cell line, and 4T1 mouse breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for E-Cadherin at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of E‑Cadherin in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.
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E‑Cadherin in Human Epidermoid Carcinoma Cells. E-Cadherin was detected in immersion fixed human epidermoid carcinoma cells using 10 µg/mL Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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E‑Cadherin and SOX2 in BG01V Human Stem Cells. E-Cadherin and SOX2 were detected in BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) and 10 µg/mL Human/Mouse SOX2 Monoclonal Antibody (Catalog # MAB2018). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for E-Cadherin using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL001) and for SOX2 using the NorthernLights 493-conjugated Anti-Mouse Secondary Antibody (red; Catalog # NL009). Cells were counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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E‑Cadherin in Human Stomach. E-Cadherin was detected in immersion fixed paraffin-embedded sections of human stomach using Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane and cytoplasm in gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Detection of Human and Mouse E‑Cadherin by Simple WesternTM. Simple Western lane view shows lysates of 4T1 mouse breast cancer cell line, P19 mouse embryonal carcinoma cell line, A431 human epithelial carcinoma cell line, and MCF-7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for E-Cadherin at approximately 128 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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E-Cadherin in Human Stomach Using Dual RNAscope®ISH and IHC. E-Cadherin mRNA was detected in formalin-fixed paraffin-embedded tissue sections of human stomach probed with ACD RNAScope®Probe (Catalog # 311091) and stained using ACD RNAscope®2.5 HD Detection Reagents-Red (top image, Catalog # 32260). Adjacent tissue section was processed for immunohistochemistry using R&D Systems Goat Anti-Human E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) at 0.3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC004) and DAB chromogen (lower image, yellow-brown). Tissues were counterstained with hematoxylin (blue).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: E-Cadherin
Epithelial (E)‑Cadherin (ECAD), also known as cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the Cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E‑Cadherin may also play a role in tumor development, as loss of E‑Cadherin has been associated with tumor invasiveness. E‑Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. E‑Cadherin contains five extracellular calcium‑binding domains of approximately 110 amino acids each.
- Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
- Overduin, M. et al. (1995) Science 267:386.
- Takeichi, M. (1991) Science 251:1451.
Product Datasheets
Citations for Human/Mouse E-Cadherin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Epithelial-derived factors induce muscularis mucosa of human induced pluripotent stem cell-derived gastric organoids
Authors: K Uehara, M Koyanagi-A, T Koide, T Itoh, T Aoi
Stem Cell Reports, 2022;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Induced organoids derived from patients with ulcerative colitis recapitulate colitic reactivity
Authors: SK Sarvestani, S Signs, B Hu, Y Yeu, H Feng, Y Ni, DR Hill, RC Fisher, S Ferrandon, RK DeHaan, J Stiene, M Cruise, TH Hwang, X Shen, JR Spence, EH Huang
Nature Communications, 2021;12(1):262.
Species: Human
Sample Types: Organoid
Applications: IHC -
Functional human gastrointestinal organoids can be engineered from three primary germ layers derived separately from pluripotent stem cells
Authors: AK Eicher, DO Kechele, N Sundaram, HM Berns, HM Poling, LE Haines, JG Sanchez, K Kishimoto, M Krishnamur, L Han, AM Zorn, MA Helmrath, JM Wells
Cell Stem Cell, 2021;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Human iPSC-derived fallopian tube organoids with BRCA1 mutation recapitulate early-stage carcinogenesis
Authors: N Yucer, R Ahdoot, MJ Workman, AH Laperle, MS Recouvreux, K Kurowski, DJ Naboulsi, V Liang, Y Qu, JT Plummer, SA Gayther, S Orsulic, BY Karlan, CN Svendsen
Cell Reports, 2021;37(13):110146.
Species: Human
Sample Types: Organoid
Applications: IHC -
A Wnt-mediated phenotype switch along the epithelial-mesenchymal axis defines resistance and invasion downstream of ionising radiation in oral squamous cell carcinoma
Authors: F Zolghadr, N Tse, D Loka, G Joun, S Meppat, V Wan, H Zoellner, M Xaymardan, CS Farah, JG Lyons, E Hau, E Patrick, N Seyedasli
British Journal of Cancer, 2021;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Patient-Derived Organotypic Epithelial Rafts Model Phenotypes in Juvenile-Onset Recurrent Respiratory Papillomatosis
Authors: MC Bedard, MG Brusadelli, A Carlile, S Ruiz-Torre, H Lodin, D Lee, M Kofron, PF Lambert, A Lane, N Ameziane, EM Bahassi, KA Wikenheise, A de Alarcon, DF Smith, SI Wells
Viruses, 2021;13(1):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Modulation of glycosyltransferase ST6Gal-I in gastric cancer-derived organoids disrupts homeostatic epithelial cell turnover
Authors: KL Alexander, CA Serrano, A Chakrabort, M Nearing, LN Council, A Riquelme, M Garrido, SL Bellis, LE Smythies, PD Smith
J. Biol. Chem., 2020;0(0):.
Species: Human
Sample Types: Organoid
Applications: ICC -
Claudin-18 Loss Alters Transcellular Chloride Flux but not Tight Junction Ion Selectivity in Gastric Epithelial Cells
Authors: TJ Caron, KE Scott, N Sinha, S Muthupalan, M Baqai, LH Ang, Y Li, JR Turner, JG Fox, SJ Hagen
Cell Mol Gastroenterol Hepatol, 2020;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Modeling Intestinal Epithelial Response to Interferon-&gamma in Induced Pluripotent Stem Cell-Derived Human Intestinal Organoids
Authors: MJ Workman, E Troisi, SR Targan, CN Svendsen, RJ Barrett
International Journal of Molecular Sciences, 2020;22(1):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Repair of acute liver damage with immune evasive hESC derived hepato-blasts
Authors: J Liu, T Pan, Y Chen, Y Liu, F Yang, Q Chen, N Abbas, M Zhong, Q Zhang, Y Xu, YX Li
Stem Cell Res, 2020;49(0):102010.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
miR-221-5p regulates proliferation and migration in human prostate cancer cells and reduces tumor growth in vivo
Authors: M Kiener, L Chen, M Krebs, J Grosjean, I Klima, C Kalogirou, H Riedmiller, B Kneitz, GN Thalmann, E Snaar-Jaga, M Spahn, M Kruithof-d, E Zoni
BMC Cancer, 2019;19(1):627.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Engineered E. coli Nissle 1917 for the delivery of matrix-tethered therapeutic domains to the gut
Authors: P Praveschot, AM Duraj-That, I Gelfat, F Bahl, DB Chou, NS Joshi
Nat Commun, 2019;10(1):5580.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
IDENTIFICATION OF PIPERAZINYLBENZENESULFONAMIDES AS NEW INHIBITORS OF CLAUDIN-1 TRAFFICKING AND HEPATITIS C VIRUS ENTRY
Authors: L Riva, OR Song, J Prentoe, F Helle, L L'homme, CH Gattolliat, A Vandeputte, L Fénéant, S Belouzard, TF Baumert, T Asselah, J Bukh, P Brodin, L Cocquerel, Y Rouillé, J Dubuisson
J. Virol., 2018;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
A novel transforming growth factor beta-induced long noncoding RNA promotes an inflammatory microenvironment in human intrahepatic cholangiocarcinoma
Authors: A Merdrignac, G Angenard, C Allain, K Petitjean, D Bergeat, P Bellaud, A Fautrel, B Turlin, B Clément, S Dooley, L Sulpice, K Boudjema, C Coulouarn
Hepatol Commun, 2018;2(3):254-269.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Enhanced Utilization of Induced Pluripotent Stem Cell-Derived Human Intestinal Organoids Using Microengineered Chips.
Authors: Michael J Workman, John P Gleeson, Elissa J Troisi, Hannah Q Estrada, S Jordan Kerns, Christopher D Hinojosa, Geraldine A Hamilton, Stephan R Targan, Clive N Svendsen, Robert J Barrett
Cellular and Molecular Gastroenterology and Hepatology, 2018;0(0):2352-345X.
Species: Human
Sample Types: Whole Cells on Intestine Chip
Applications: ICC -
Acoustic Tweezing Cytometry Induces Rapid Initiation of Human Embryonic Stem Cell Differentiation
Authors: T Topal, X Hong, X Xue, Z Fan, N Kanetkar, JT Nguyen, J Fu, CX Deng, PH Krebsbach
Sci Rep, 2018;8(1):12977.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Placenta-specific protein 1 promotes cell proliferation and invasion in non-small cell lung cancer
Authors: L Yang, TQ Zha, X He, L Chen, Q Zhu, WB Wu, FQ Nie, Q Wang, CS Zang, ML Zhang, J He, W Li, W Jiang, KH Lu
Oncol. Rep., 2018;39(1):53-60.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Surface Topography Guides Morphology and Spatial Patterning of Induced Pluripotent Stem Cell Colonies
Authors: G Abagnale, A Sechi, M Steger, Q Zhou, CC Kuo, G Aydin, C Schalla, G Müller-New, M Zenke, IG Costa, P van Rijn, A Gillner, W Wagner
Stem Cell Reports, 2017;9(2):654-666.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Directed Differentiation of Human Induced Pluripotent Stem Cells into Fallopian Tube Epithelium
Authors: N Yucer, M Holzapfel, T Jenkins Vo, L Lenaeus, L Ornelas, A Laury, D Sareen, R Barrett, BY Karlan, CN Svendsen
Sci Rep, 2017;7(1):10741.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Novel role for IL-22 in protection during chronic Mycobacterium tuberculosis HN878 infection
Authors: P Treerat, O Prince, A Cruz-Lagun, M Muñoz-Torr, MA Salazar-Le, M Selman, B Fallert-Ju, TA Reinhardt, JF Alcorn, D Kaushal, J Zuñiga, J Rangel-Mor, JK Kolls, SA Khader
Mucosal Immunol, 2017;0(0):.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Tissue
Applications: IHC -
miR-151a induces partial EMT by regulating E-cadherin in NSCLC cells
Authors: I Daugaard, KJ Sanders, A Idica, K Vittayaruk, M Hamdorf, JD Krog, R Chow, D Jury, LL Hansen, H Hager, P Lamy, CL Choi, D Agalliu, DG Zisoulis, IM Pedersen
Oncogenesis, 2017;6(7):e366.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Wnt/?-catenin promotes gastric fundus specification in mice and humans
Authors: KW McCracken, E Aihara, B Martin, CM Crawford, T Broda, J Treguier, X Zhang, JM Shannon, MH Montrose, JM Wells
Nature, 2017;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
A process engineering approach to increase organoid yield
Authors: N Arora, J Imran Also, JW Guggenheim, M Mak, J Munera, JM Wells, RD Kamm, HH Asada, SY Shvartsman, LG Griffith
Development, 2017;144(6):1128-1136.
Species: Human
Sample Types: Spheroid
Applications: IHC -
Canine spontaneous head and neck squamous cell carcinomas represent their human counterparts at the molecular level.
Authors: Liu D, Xiong H, Ellis A, Northrup N, Dobbin K, Shin D, Zhao S
PLoS Genet, 2015;11(6):e1005277.
Species: Canine
Sample Types: Whole Cells
Applications: IHC-P -
Molecular homology and difference between spontaneous canine mammary cancer and human breast cancer.
Authors: Liu D, Xiong H, Ellis A, Northrup N, Rodriguez C, O'Regan R, Dalton S, Zhao S
Cancer Res, 2014;74(18):5045-56.
Species: Canine
Sample Types: Whole Tissue
Applications: IHC-P -
Penton-dodecahedral particles trigger opening of intercellular junctions and facilitate viral spread during adenovirus serotype 3 infection of epithelial cells.
Authors: Lu Z, Wang H, Zhang Y, Cao H, Li Z, Fender P, Lieber A
PLoS Pathog, 2013;9(10):e1003718.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Interleukin-15 plays a central role in human kidney physiology and cancer through the gamma c signaling pathway.
Authors: Giron-Michel J, Azzi S, Khawam K, Mortier E, Caignard A, Devocelle A, Ferrini S, Croce M, Francois H, Lecru L, Charpentier B, Chouaib S, Azzarone B, Eid P
PLoS ONE, 2012;7(2):e31624.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Snail involves in the transforming growth factor beta1-mediated epithelial-mesenchymal transition of retinal pigment epithelial cells.
Authors: Li H, Wang H, Wang F, Gu Q, Xu X
PLoS ONE, 2011;6(8):e23322.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Tissue engineering of oral dysplasia.
Authors: Gaballah K, Costea DE, Hills A, Gollin SM, Harrison P, Partridge M
J. Pathol., 2008;215(3):280-9.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Downregulation of Rap1GAP contributes to Ras transformation.
Authors: Tsygankova OM, Prendergast GV, Puttaswamy K, Wang Y, Feldman MD, Wang H, Brose MS, Meinkoth JL
Mol. Cell. Biol., 2007;27(19):6647-58.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Requirement for intercellular adhesion molecule 1 and caveolae in invasion of human oral epithelial cells by Porphyromonas gingivalis.
Authors: Tamai R, Asai Y, Ogawa T
Infect. Immun., 2005;73(10):6290-8.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Human Pluripotent Stem Cell-Derived Kidney Model for Nephrotoxicity Studies.
Authors: Bajaj P, Rodrigues A, Steppan C, Engle S, Mathialagan S, Schroeter T
Drug Metab Dispos, 0;46(11):1703-1711.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Novel human renal proximal tubular cell line for the production of complex proteins.
Authors: Fliedl L, Manhart G, Kast F, Katinger H, Kunert R, Grillari J, Wieser M, Grillari-Voglauer R
J Biotechnol, 0;176(0):29-39.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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Reviews for Human/Mouse E-Cadherin Antibody
Average Rating: 3.8 (Based on 5 Reviews)
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The antibody did not give any specific staining on mouse paraffin sections of choroid plexus, Epitope retrieval: Sodium citrate
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***
Antigen retrieval: 10mM Citrate pH6
Antibody dilution: 1:400
Secondary antibody: Alexa 647 donkey anti-goat secondary antibody
Antigen retrieval: 10mM Citrate buffer pH6.
Antibody dilution: 1:400
Secondary antibody: CY3 conjugated donkey anti-goat secondary antibody @ 1:300
