Cultrex Cell Invasion Assays contain all the reagents necessary to investigate cell invasion through different extracellular matrices and matrix components. Different cell lines and different treatments can result in a wide range of invasive potentials. Thus, these assays offer researchers the choice of several different extracellular matrix proteins with which to coat the PET membranes of the migration chamber and the ability to adjust the coating concentration so to optimize the permissiveness of the matrix for each experiment. Cultrex Cell Invasion Assays are available to assess cell invasion against Laminin I, Collagen I, Collagen IV, and Basement Membrane Extract (BME).
FBS Stimulates Migration of NIH-3T3 and HT1080 Cells. The NIH-3T3 mouse embryonic fibroblast cell line and the HT1080 human fibrosarcoma cell line were treated with 10% fetal bovine serum (FBS). The migration of untreated (yellow bars) and treated (green bars) NIH-3T3 and HT1080 cells against different extracellular matrix components, including BME, Laminin I, Collagen I, Collagen IV, were quantified using the Cultrex Cell Invasion Assay Kits (Catalog # 3455-096-K, 3456-096-K, 3457-096-K, 3458-096-K, respectively). Data from four experiments was quantified for both non-invasive (NIH-3T3) and invasive (HT1080) cell types.