CX3CR1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-76872
![Western Blot: CX3CR1 AntibodyBSA Free [NBP1-76872]](https://resources.rndsystems.com/images/products/CX3CR1-Antibody-Western-Blot-NBP1-76872-img0006.jpg "Western Blot: CX3CR1 AntibodyBSA Free [NBP1-76872]")
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (93%), Rabbit (93%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Antibody was raised against a peptide corresponding to amino acids in a extracellular loop of human CX3CR1. The immunogen is located within amino acids 160 - 210 of CX3CR1.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (86%).
Specificity
At least two isoforms of CX3CR1 are known to exist.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CX3CR1 Antibody - BSA Free
Western Blot: CX3CR1 AntibodyBSA Free [NBP1-76872]
Western Blot: CX3CR1 Antibody [NBP1-76872] - Analysis of CX3CR1 in THP-1 cell lysate in the absence (lane A) or presence (lane B) of blocking peptide with anti-CX3CR1 (EL) using NBP1-76872 at 1:500 dilution.
Immunocytochemistry/ Immunofluorescence: CX3CR1 Antibody - BSA Free [NBP1-76872] -
Immunocytochemistry/ Immunofluorescence: CX3CR1 Antibody - BSA Free [NBP1-76872] - Immunofluorescence of CX3CR1 in THP-1 cells with CX3CR1 antibody at 20 ug/mL.
Immunohistochemistry: CX3CR1 Antibody - BSA Free [NBP1-76872] -
Immunohistochemistry: CX3CR1 Antibody - BSA Free [NBP1-76872] - Immunohistochemistry of CX3CR1 in human spleen cells with CX3CR1 antibody at 10 ug/mL.Applications for CX3CR1 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
20 ug/ml
Immunohistochemistry
10 ug/ml
Immunohistochemistry-Paraffin
10 ug/ml
Western Blot
0.5 ug/ml
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CX3CR1
Long Name
CX3C Chemokine Receptor 1
Alternate Names
CMKbRL1, V28
Gene Symbol
CX3CR1
UniProt
Additional CX3CR1 Products
Product Documents for CX3CR1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CX3CR1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for CX3CR1 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for CX3CR1 Antibody - BSA Free
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Q: I'm interested in antibodies against CX3CR1 to use them for IHC-Paraffin protocol in humans. I choose Your product with catalog number NBP1-76872 (0.1 mg). My question is about additional reagents, I maybe need them to perform the staining. Could you tell me whether control probe is needed? Could I receive appropriate protocol with aforesaid antibodies using?
A:
Our lab did not use any customized protocol for validation of NBP1-76872 and our general IHC-P protocol may be found at following web-link: Immunohistochemistry-Paraffin Protocol. Because this target is a membrane protein, we would recommend to not to over-fix tissues (approximately 12 hours fixation should be fine and should not exceed 24 hours). Antigen retrieval may be required for this target. It is expressed in lymphoid as well as neural tissues. Spleen and Tonsil tissue would be a nice positive control. We have CX3CR1 Peptide (NBP1-76872PEP) also that you may use for including a blocking negative control in your staining for proving specificity of the antibody.
