DuoSet® IC (IntraCellular) Assay Development Systems

DuoSet IC ELISA Reagents are sensitive and convenient assays used to measure intracellular protein levels in cell lysates. Extensive validation work is done to ensure specificity, and detailed protocols are provided. R&D Systems offers DuoSet IC assays to measure kinases, apoptosis-related and genotoxic stress proteins, heat shock proteins, and more. These signal transduction assays make an excellent alternative to Western blot, especially when used in combination with R&D Systems DuoSet IC Phospho-specific ELISAs.


  • Optimized capture and detection antibody pairings and recommended concentrations save lengthy development time
  • Mass-calibrated standards allow for consistent results and eliminate vial-to-vial variation
  • Generic development protocols provide direction to start an optimization protocol
  • Customize the assay to your specific needs
  • Available in 2, 5, and 15-(96-well) plate pack sizes
  • Excellent alternative to Western blot

Kit Contents

Assay Principle

View Full Assay Principle

Kit contents may vary slightly. Please refer to product-specific inserts for a complete list of contents for individual assays.

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard
  • Streptavidin-HRP