eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) - BSA Free

Novus Biologicals | Catalog # NBP3-26427

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1C6 expressed in HEK293

Format

BSA Free
View all eIF2 alpha Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human eIF2 alpha/EIF2S1 [p Ser51] [UniProt P05198]

Modification

p Ser 51

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) - BSA Free

eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6)

Immunohistochemistry: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] -

Immunohistochemistry: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] - Image of eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6)

Immunohistochemistry: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] -

Immunohistochemistry: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] - Image of eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6)

Western Blot: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] -

Western Blot: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] - Positive Western Blot detected in Hela whole cell lysate (treated with Calyculin A or EGF).
All lanes: eIF2 alpha/EIF2S1 [p Ser51] Antibody at 1.48ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 36 KDa
Observed band size: 36 KDa
eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6)

Immunocytochemistry/Immunofluorescence: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] -

Immunocytochemistry/Immunofluorescence: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] - Staining of A549 cells (treated with 100ng/ml EGF for 20min) with eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: eIF2 alpha

eIFs are heterotrimeric initiation complexes that form on the ribosome during protein synthesis. Each eIF serves a distinct function during protein synthesis to help attach, assemble, and drive the ribosome along the mRNA.

Long Name

Eukaryotic Translation Initiation Factor 2 Alpha

Alternate Names

EIF2S1

Gene Symbol

EIF2S1

Additional eIF2 alpha Products

Product Documents for eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) - BSA Free

Certificate of Analysis

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Product Specific Notices for eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Translation Regulation

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Protocols

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