Endoglin/CD105 Antibody (MEM-229) - BSA Free

Novus Biologicals | Catalog # NB110-58718

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Porcine, Canine (Negative), Equine (Negative)

Cited:

Porcine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Cited:

Immunohistochemistry, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # MEM-229

Format

BSA Free
View all Endoglin/CD105 Primary Antibodies »

Product Specifications

Immunogen

recombinant vaccinia virus containing human CD105 (L-isoform) cDNA

Marker

Neo-endothelial Cells Marker

Specificity

This antibody (clone MEM-229) recognizes CD105 (Endoglin), a 180 kDa type I integral membrane homodimer glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow; it is also present on syncytiotrophoblast on placenta throughout pregnancy.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Endoglin/CD105 Antibody (MEM-229) - BSA Free

Immunocytochemistry/ Immunofluorescence: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]

Immunocytochemistry/ Immunofluorescence: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]

Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Staining of an infarcted porcine heart with anti-CD105 (MEM-229; green); cell nuclei stained with DAPI (blue).
Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]

Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]

Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Staining of CD105 on Huvec cells with anti-CD105 (MEM-229) PerCP-CyTM5.5.
Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]

Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]

Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Analysis of the APC conjugate of this antibody. A cell surface stain was performed on THP-1 cells with Endoglin antibody NB110-58718APC (blue) along with a matched isotype control (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at RT.
Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]

Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]

Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - A cell surface stain was performed on HeLa cells with Endoglin/CD105 antibody (MEM-229) NB110-58718PE (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]

Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]

Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Staining of CD105 on Huvec cells with anti-CD105 (MEM-229) PE.
Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]

Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]

Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Staining of CD105 on Huvec cells with anti-CD105 (MEM-229) biotin.
Endoglin/CD105 Antibody (MEM-229) - BSA Free Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]

Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]

Separation of HUVEC cells stained using anti-human CD105 (MEM-229) purified antibody (concentration in sample 3 ug/ml, red-filled, GAM APC) from HUVEC cells stained using mouse IgG1 isotype control (MOPC-21) purified antibody (concentration in sample 3 ug/ml, black-dashed, GAM APC) in Analysis (surface staining).

Applications for Endoglin/CD105 Antibody (MEM-229) - BSA Free

Application
Recommended Usage

Flow Cytometry

2-6 ug/ml

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:200

Western Blot

1:1000
Application Notes
Western Blot: use non-reducing conditions. This antibody is CyTOF ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Endoglin/CD105

CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.

Alternate Names

CD105, ENG

Gene Symbol

ENG

Additional Endoglin/CD105 Products

Product Documents for Endoglin/CD105 Antibody (MEM-229) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Endoglin/CD105 Antibody (MEM-229) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Endoglin/CD105 Antibody (MEM-229) - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Endoglin/CD105 Antibody (MEM-229) - BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?

    A: We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.

  • Q: We are interested in CD105 antibodies that could react with dog, please let me know which products in your catalog you would recommend.

    A:

    We do not currently carry any CD105 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD105 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

  • Q: I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?

    A: We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.

  • Q: We are interested in CD105 antibodies that could react with dog, please let me know which products in your catalog you would recommend.

    A:

    We do not currently carry any CD105 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD105 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

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