EpCAM/TROP1 Antibody (28) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-89605

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA Detection, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 28 expressed in HEK293

Format

Azide and BSA Free
View all EpCAM/TROP1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human EpCAM/TROP1 (Accession#: NP_002345.1; Met 1-Lys 265).

Specificity

No cross-reactivity in ELISA with: Human CD146 / MCAMHuman CD171 / N-CAML1 / L1CAM Human OBCAM / OPCML Human TROP2 / TACSTD2

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for EpCAM/TROP1 Antibody (28) - Azide and BSA Free

Western Blot: EpCAM/TROP1 Antibody (28) [NBP2-89605]

Western Blot: EpCAM/TROP1 Antibody (28) [NBP2-89605]

Western Blot: EpCAM/TROP1 Antibody (28) [NBP2-89605] - Lane A: MCF-7 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:34 kDa Observed band size:38 kDa
Immunocytochemistry/ Immunofluorescence: EpCAM/TROP1 Antibody (28) [NBP2-89605]

Immunocytochemistry/ Immunofluorescence: EpCAM/TROP1 Antibody (28) [NBP2-89605]

Immunocytochemistry/Immunofluorescence: EpCAM/TROP1 Antibody (28) [NBP2-89605] - Staining of Human EpCAM in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human EpCAM monoclonal antibody (1:500) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.
Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (28) [NBP2-89605]

Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (28) [NBP2-89605]

Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (28) [NBP2-89605] - Human mammary gland with rabbit monoclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of alveolus epithelium.
Flow Cytometry: EpCAM/TROP1 Antibody (28) [NBP2-89605]

Flow Cytometry: EpCAM/TROP1 Antibody (28) [NBP2-89605]

Flow Cytometry: EpCAM/TROP1 Antibody (28) [NBP2-89605] - Analysis of anti-EpCAM (CD326) reactivity on SKBR3 cells. SKBR3 cells were stained with anti-EpCAM (CD326) Monoclonal Ab, then a FITC-conjugated second step antibody. The histogram were derived from the gated events based on light scattering characteristics of viable cells.
Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (28) [NBP2-89605]

Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (28) [NBP2-89605]

Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (28) [NBP2-89605] - Staining of human EpCAM in human colon carcinoma with rabbit monoclonal antibody at 1:2500 dilution. Positive staining was localized to membrane of colonic gland epithelium.
Immunoprecipitation: EpCAM/TROP1 Antibody (28) [NBP2-89605]

Immunoprecipitation: EpCAM/TROP1 Antibody (28) [NBP2-89605]

Immunoprecipitation: EpCAM/TROP1 Antibody (28) [NBP2-89605] - Lane A:0.5 mg MCF-7 Whole Cell Lysate Lane B:0.5 mg A431 Whole Cell LysateLane C:0.5 mg HepG2 Whole Cell Lysate Lane D:0.5 mg Caco-2 Whole Cell Lysate 0.5 uL anti-EpCAM rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-EpCAM rabbit monoclonal antibody,at 1:5000 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 40 kDa Observed band size: 40 kDa
EpCAM/TROP1 Antibody (28) - Azide and BSA Free

Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (28) [NBP2-89605] -

Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (28) [NBP2-89605] - Staining of human EpCAM/TROP1 in human bladder carcinoma with NBP2-89605 at 1:2500 dilution. Positive staining was localized to membrane of transitional epithelium.

Applications for EpCAM/TROP1 Antibody (28) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:25000-1:50000

Flow Cytometry

1:100-1:500

Immunocytochemistry/ Immunofluorescence

1:250-1:5000

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

0.2-1 ul/mg of lysate

Sandwich ELISA Detection

1:5000-1:50000

Western Blot

1:500-1:1000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EpCAM/TROP1

Epithelial cell adhesion molecule (EpCAM), also known as TROP1 and CD326, is a type I transmembrane glycoprotein that plays a role in a number of cellular processes including adhesion, signaling, maintaining stemness, proliferation, migration, and invasion (1,2). The human EpCAM protein is 314 amino acids (aa) in length with a theoretical molecular weight (MW) of ~35 kDa (1,3,4). The EpCAM protein includes a signal peptide, an extracellular N-terminal domain, a thyroglobulin type-1 domain, a carboxyl-terminal domain, a single-pass transmembrane domain, and an intracellular domain (1,3). The protein is highly conserved and has approximately 81% aa sequence identity between human and mouse (5). Trophoblast cell-surface antigen 2 (TROP2) also shares ~67% aa sequence similarity with EpCAM (1,5). While both EpCAM and TROP2 are cell surface markers expressed in the epithelium, their expression levels typically do not correlate (5). EpCAM is expressed in normal epithelial tissue and elevated expression is observed in many epithelial carcinomas (1-3) Patient tumor samples with increased EpCAM expression have been associated with poor prognosis (1). Given EpCAM's elevated expression in tumors, it has become a potential target for cancer therapy approaches, including monoclonal antibody treatment and anti-EpCAM trispecific antibodies (1,5).

EpCAM functions as an intracellular signaling molecule and contributes to regulation of epithelial-to-mesenchymal (EMT) transition (4,5). EpCAM is cleaved during the process of regulated intramembrane proteolysis (RIP) (4,5). Initial cleavage occurs at the membrane via a disintegrin and metalloprotease 17 (ADAM17) which releases EpCAM's extracellular domain (EpEx) (4,5). A secondary cleavage is mediated by presenilin 2 (PSEN2) which releases EpCAM's cytoplasmic trail (EpICD) (4,5). EpICD translocates to the nucleus where it associates with beta-catenin, FHL-2, and LEF-1 and induces transcription of genes related to EMT and tumor growth (4,5). EpCAM has also been shown to regulate structure and functionality of the apical junction complex of cells through direct interaction with claudin-7 and association with E-cadherin (2,5). Loss of EpCAM disrupts adherins junction and tight junction structure and function (2).

References

1. Mohtar MA, Syafruddin SE, Nasir SN, Low TY. Revisiting the Roles of Pro-Metastatic EpCAM in Cancer. Biomolecules. 2020; 10(2):255. https://doi.org/10.3390/biom10020255

2. Huang L, Yang Y, Yang F, et al. Functions of EpCAM in physiological processes and diseases (Review). Int J Mol Med. 2018; 42(4):1771-1785. https://doi.org/10.3892/ijmm.2018.3764

3. Brown TC, Sankpal NV, Gillanders WE. Functional Implications of the Dynamic Regulation of EpCAM during Epithelial-to-Mesenchymal Transition. Biomolecules. 2021; 11(7):956. https://doi.org/10.3390/biom11070956

4. Uniprot (P16422)

5. Schnell U, Cirulli V, Giepmans BN. EpCAM: structure and function in health and disease. Biochim Biophys Acta. 2013; 1828(8):1989-2001. https://doi.org/10.1016/j.bbamem.2013.04.018

Long Name

Epithelial Cell Adhesion Molecule

Alternate Names

17-1A, CD326, GA733-2, gp40, KS1/4, M4S1, TACSTD1, TROP1

Gene Symbol

EPCAM

Additional EpCAM/TROP1 Products

Product Documents for EpCAM/TROP1 Antibody (28) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for EpCAM/TROP1 Antibody (28) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs for EpCAM/TROP1 Antibody (28) - Azide and BSA Free

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  • Q: I'm looking for EpCAM antibody for rat. Do you have any antibody which can use FACS?

    A:

    We unfortunately do not have any EpCAM antibodies validated in FACS for detection of the rat protein. I am sorry for any inconvenience. I do have three products to EpCAM that can detect the rat protein. These products have been validated to detect the native rat protein, so they may work for FACS however they have not yet been tested in that particular application. If you would like to try them in your experiment you would qualify for our Innovator's Reward Program. This program was designed to help scientists with the cost of their antibodies in return for valuable information. If you decide to test an antibody in a previously untested species or application we will issue you a credit for the purchase price of the antibody in exchange for a review of your experiment using our product.

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