ERK1 Inhibitor Peptide Set

Novus Biologicals | Catalog # NBP2-29333

Novus Biologicals
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Key Product Details

Species

Human, Mouse, Rat, Hamster, Rabbit, Xenopus

Product Specifications

Specificity

The ERK inhibitory peptide also contains a protein transduction (PTD) sequence (DRQIKIWFQNRRMKWKK) derived from antennapedia which renders the peptide cell permeable.

The control peptide consists of only the PTD sequence.

Application Notes

Inhibition of Erk activation.
The inhibitor peptide is to block ERK activation by MEK. Optimal peptide concentrations and incubation times vary between model systems and should be determined empirically by users. A 100 uM final concentration may be a useful starting point.

Please refer to Kalemen et al (2002) for additional information about how the inhibitor peptide has been used to block ERK activation by MEK.

Reactivity Notes

Broad; the peptide sequence is 100% conserved among multiple species.

Inhibitor Content

ERK Inhibitor peptide: 2 x 1 mg (lyophilized) DRQIKIWFQNRRMKWKKGMPKKKPTPIQLN (ERK inhibitor sequence: GMPKKKPTPIQLN). Molecular weight: 3795. Antennapedia Control peptide: 2 x 1 mg (lyophilized) DRQIKIWFQNRRMKWKK. Molecular weight: 2361

Formulation, Preparation, and Storage

Preparation Method

Preparation of 5 mM Stock Solutions
PBS* is added directly to the vials to prepare the stock solutions. Note: Bring the solution to room temperature and quick spin the tubes before opening the caps.

ERK Inhibitor Peptide: 1 mg of DRQIKIWFQNRRMKWKKGMPKKKPTPIQLN
Add 53 ul of PBS* to the vial to make a 5 mM stock solution. Mix by vortexing. Aliquot and store at -20C or -80C. Avoid repeated freeze thawing.

Control Peptide: 1 mg of DRQIKIWFQNRRMKWKK
Add 84.8 ul PBS* to the vial. Mix by vortexing. Aliquot and store at 20C or -80C. Avoid repeated freeze thawing.
Recipe for 1X PBS:
1. Dissolve the following in 800ml distilled H2O.
-8g of NaCl
-0.2g of KCl
-1.44g of Na2HPO4
-0.24g of KH2PO4
2. Adjust pH to 7.5 with HCl.
3. Adjust volume to 1L with additional distilled H2O.
4. Sterilize by autoclaving.

Please contact technical support for detailed reconstitution instructions.

Concentration

Lyoph

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ERK1

ERK (extracellular signal-regulated kinase) is a member of the Mitogen-activated protein kinases (MAPK) family of protein kinases that are essential for cellular proliferation and differentiation. The activation of MAPKs requires a cascade mechanism whereby MAPK is phosphorylated by an upstream kinase MAPKK (MEK) which is then, in turn phosphorylated by a third kinase MAPKKK (MEKK). This inhibitory peptide contains the amino-terminal 13 amino acids (GMPKKKPTPIQLN) of MEK1 and binds to ERK.1. This blocks ERK activation by MEK as ERK is unable to interact with MEK.

Long Name

Extracellular Signal-regulated Kinase 1

Alternate Names

MAPK3, P44ERK1, p44mapk, PRKM3

Gene Symbol

MAPK3

Additional ERK1 Products

Product Documents for ERK1 Inhibitor Peptide Set

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for ERK1 Inhibitor Peptide Set

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Inhibitors are guaranteed for 1 year from date of receipt.

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Protocols

View specific protocols for ERK1 Inhibitor Peptide Set (NBP2-29333):

ERK1 Inhibitor Peptide Set:
Hazard Information
Chemical Name: Non hazardous products
Chemical Formula: N/A
CAS Number: N/A
EEC-No: N/A

Hazard Identification
None
First Aid Measures
Eye Contact: None
Skin Contact: None
Inhalation: None
Ingestion: None

Accidental Release Measures
This product either does not contain hazardous constituents or the concentration of all chemical constituents are below the regulatory threshold limits described by Occupational Safety Health Administration Hazard Communication Standard 29 CFR 1910.1200 and the European Directive 91/155/EEC. 88/379/EEC, and 67/546/EEC.

Handling and Storage
Exposure Controls / Personal Protection
Other Precautions: None

Physical and Chemical Properties
Form: N/A
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Odor: N/A
Melting Point: N/A
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Solubility in Water: N/A
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Explosion limits: N/A
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