FGF-4 (Fibroblast Growth Factor-4), also known as FGF-K and K-FGF, is a secreted angiogenic factor that is mitogenic for fibroblasts and endothelial cells. During development, it is required for maintenance of the trophoectoderm and primitive endoderm, and it cooperates with FGF-8 in the apical ectodermal ridge. FGF-4 promotes embryonic stem cell proliferation and may also aid stem cell differentiation. A C-terminally truncated 15 kDa isoform that opposes full-length FGF-4 and promotes differentiation is endogenously expressed in human embryonic stem cells.
FGF-4 Antibody [Allophycocyanin]
Novus Biologicals | Catalog # AF235A
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Neutralization
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Polyclonal Goat IgG
Loading...
Product Specifications
Immunogen
E. coli-derived recombinant human FGF-4
Ala31-Leu206
Accession # P08620
Ala31-Leu206
Accession # P08620
Specificity
Detects human FGF‑4 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) FGF-6 is observed and less than 2% cross-reactivity with bovine FGF acidic, bovine FGF basic, rhFGF acidic, rhFGF basic, rh beta ‑ECGF, rhFGF-5, rhFGF‑7, rhFGF‑8b, recombinant mouse (rm) FGF‑8b, rmFGF‑8c, and rhFGF‑10 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for FGF-4 Antibody [Allophycocyanin]
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Neutralization
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: FGF-4
Long Name
Fibroblast Growth Factor 4
Alternate Names
FGF4, HBGF-4, HST-1, KFGF, KS3
Gene Symbol
FGF4
Additional FGF-4 Products
Product Documents for FGF-4 Antibody [Allophycocyanin]
Product Specific Notices for FGF-4 Antibody [Allophycocyanin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for FGF-4 Antibody [Allophycocyanin]
There are currently no reviews for this product. Be the first to review FGF-4 Antibody [Allophycocyanin] and earn rewards!
Have you used FGF-4 Antibody [Allophycocyanin]?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
