Human FGF-4 Antibody Summary
Accession # P08620
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by FGF‑4 and Neutralization by Human FGF‑4 Antibody. Recombinant Human FGF-4 (Catalog # 235-F4) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF-4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF235). The ND50 is typically 0.3-0.9 µg/mL.
FGF‑4 in Human Breast Cancer Tissue. FGF‑4 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human FGF‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF235) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
FGF-4, the product of a developmentally regulated gene (hst-1), is a member of the FGF family that is efficiently secreted. The gene for FGF-4 (also known as FGFK or K-FGF for Kaposi sarcoma-associated FGF) was initially discovered as a transforming gene by the NIH-3T3 focus formation assay using DNA derived from human tumors (including stomach and colon cancers, hepatocellular carcinomas, and Kaposi’s sarcomas). FGF-4 does not seem to be expressed in normal adult tissues. However, expression of the gene is spatially and temporally regulated during embryonic development. The murine homologue of human FGF-4 has been cloned and shown to be 82% homologous to the human protein at the amino acid sequence level. Human FGF-4 has been shown to exhibit cross species activity.
In vitro, FGF-4 is mitogenic for fibroblasts and endothelial cells. FGF-4 has been shown to be a potent angiogenesis promoter in vivo. FGF-4 has potent transforming potential apparently through an autocrine mechanism of action. FGF-4 plays a key role in limb development and has been identified as the molecular mediator of the activities of the apical ectodermal ridge that is required for directing the outgrowth and patterning of vertebrate limbs.
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