FoxO1/FKHR Antibody - BSA Free
Novus Biologicals | Catalog # NB100-2312
![Western Blot: FoxO1/FKHR Antibody [NB100-2312]](https://resources.rndsystems.com/images/products/FoxO1-FKHR-Antibody-Western-Blot-NB100-2312-img0016.jpg "Western Blot: FoxO1/FKHR Antibody [NB100-2312]")
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Scientific Data Images for FoxO1/FKHR Antibody - BSA Free
Western Blot: FoxO1/FKHR Antibody [NB100-2312]
Western Blot: FoxO1/FKHR Antibody [NB100-2312] - Whole cell lysate (50 ug) from Hep-G2, HeLa, HEK293T, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-FOXO1a antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunoprecipitation: FoxO1/FKHR Antibody [NB100-2312]](https://resources.rndsystems.com/images/products/FoxO1-FKHR-Antibody-Immunoprecipitation-NB100-2312-img0015.jpg "Immunoprecipitation: FoxO1/FKHR Antibody [NB100-2312]")
Immunoprecipitation: FoxO1/FKHR Antibody [NB100-2312]
Immunoprecipitation: FoxO1/FKHR Antibody [NB100-2312] - Detection of human FOXO1a by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FOXO1a antibody NB100-2312 (lot NB100-2312-3) used for IP at 6 ug per reaction. FOXO1a was also immunoprecipitated by a previous lot of this antibody (lot NB100-2312-2). For blotting immunoprecipitated FOXO1a, NB100-2312 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for FoxO1/FKHR Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence
Immunoprecipitation
Western Blot
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Background: FoxO1/FKHR
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Additional FoxO1/FKHR Products
Product Documents for FoxO1/FKHR Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for FoxO1/FKHR Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Citations for FoxO1/FKHR Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for FoxO1/FKHR Antibody - BSA Free
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Q: I've bought 2 antibodies from novus biologicals (NB100-614, NB100-2312) and now i am optimizing them for IP. Which beads did you use, protein a or protein g beads? in some cases only one kind of beads work, i have now tried protein a beads. If possible it would be also very helpful which protocol you used for the IP? (buffers, volumes, incubation times?)
A: Protein A beads were used for IP for these products. We don't have a specific protocol. We recommend for NB100-2312 a dilution of 1 to 10 mcg/mg lysate. For NB100-614 the dilution is 2 to 5 ug/mg lysate. Please see our general IP protocol.
Associated Pathways
