Frizzled-2 Antibody

Novus Biologicals | Catalog # NBP1-20920

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Peptide with sequence RYATLEHPFHCPR corresponding to internal region according to NP_001457.1.

Epitope

internal region

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24635767).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Frizzled-2 Antibody

Western Blot: Frizzled-2 Antibody [NBP1-20920]

Western Blot: Frizzled-2 Antibody [NBP1-20920]

Western Blot: Frizzled-2 Antibody [NBP1-20920] - (0.03ug/ml) staining of Human Heart (A) and (0.1ug/ml) Placenta (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Frizzled-2 Antibody [NBP1-20920]

Immunocytochemistry/ Immunofluorescence: Frizzled-2 Antibody [NBP1-20920]

Immunocytochemistry/Immunofluorescence: Frizzled-2 Antibody [NBP1-20920] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunohistochemistry-Paraffin: Frizzled-2 Antibody [NBP1-20920]

Immunohistochemistry-Paraffin: Frizzled-2 Antibody [NBP1-20920]

Immunohistochemistry-Paraffin: Frizzled-2 Antibody [NBP1-20920] - Staining of paraffin embedded human thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: Frizzled-2 Antibody [NBP1-20920]

Immunohistochemistry-Paraffin: Frizzled-2 Antibody [NBP1-20920]

Immunohistochemistry-Paraffin: Frizzled-2 Antibody [NBP1-20920] - Staining of paraffin embedded human liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Frizzled-2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

2 - 5 ug/ml

Immunohistochemistry-Paraffin

2 - 5 ug/ml

Peptide ELISA

Detection limit 1:64000

Western Blot

0.03-1 ug/ml
Application Notes
WB: Preliminary experiments gave an approx. 23 kDa band in human colon, heart and ovary lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 63.6 kDa band according to NP_001457.1. The 23 kDa band was successfully blocked by incubation with the immunizing peptide. IHC: Human thyroid gland shows cytoplasm and membranous staining in epithelial cells of the follicles. IHC successful usage on human glioblastomas is reported in scientific literature (PMID: 23748645).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Frizzled-2

Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The expression of the FZD2 gene appears to be developmentally regulated, with high levels of expression in fetal kidney and lung and in adult colon and ovary.

Alternate Names

Frizzled2, FZD2

Entrez Gene IDs

2535 (Human); 57265 (Mouse); 64512 (Rat)

Gene Symbol

FZD2

UniProt

Q14332 (Human)

Additional Frizzled-2 Products

Product Documents for Frizzled-2 Antibody

Certificate of Analysis

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Product Specific Notices for Frizzled-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Frizzled-2 Antibody

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Protocols

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