GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-70791
![Western Blot: GAD1/GAD67 Antibody (OTI3G9)Azide and BSA Free [NBP2-70791]](https://resources.rndsystems.com/images/products/GAD1-GAD67-Antibody-OTI3G9-Azide-and-BSA-Free-Western-Blot-NBP2-70791-img0002.jpg "Western Blot: GAD1/GAD67 Antibody (OTI3G9)Azide and BSA Free [NBP2-70791]")
Key Product Details
Species Reactivity
Human, Mouse, Rat, Monkey, Zebrafish
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI3G9
Format
Azide and BSA Free
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Product Specifications
Immunogen
Full length human recombinant protein of human GAD1 (NP_000808) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
66.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free
Western Blot: GAD1/GAD67 Antibody (OTI3G9)Azide and BSA Free [NBP2-70791]
Western Blot: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GAD1/GAD67 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAD1/GAD67.![Immunocytochemistry/ Immunofluorescence: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]](https://resources.rndsystems.com/images/products/GAD1-GAD67-Antibody-OTI3G9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70791-img0003.jpg "Immunocytochemistry/ Immunofluorescence: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]")
Immunocytochemistry/ Immunofluorescence: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]
Immunocytochemistry/Immunofluorescence: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791] - Staining of A549 cells using anti-GAD1/GAD67 mouse monoclonal antibody.![Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]](https://resources.rndsystems.com/images/products/GAD1-GAD67-Antibody-OTI3G9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70791-img0012.jpg "Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]")
Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]
Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791] - Staining of paraffin-embedded Human pancreas tissue using anti-GAD1/GAD67 mouse monoclonal antibody.![Western Blot: GAD1/GAD67 Antibody (OTI3G9)Azide and BSA Free [NBP2-70791]](https://resources.rndsystems.com/images/products/GAD1-GAD67-Antibody-OTI3G9-Azide-and-BSA-Free-Western-Blot-NBP2-70791-img0001.jpg "Western Blot: GAD1/GAD67 Antibody (OTI3G9)Azide and BSA Free [NBP2-70791]")
Western Blot: GAD1/GAD67 Antibody (OTI3G9)Azide and BSA Free [NBP2-70791]
Western Blot: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GAD1/GAD67 monoclonal antibody.![Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]](https://resources.rndsystems.com/images/products/GAD1-GAD67-Antibody-OTI3G9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70791-img0004.jpg "Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]")
Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]
Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GAD1/GAD67 mouse monoclonal antibody.![Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]](https://resources.rndsystems.com/images/products/GAD1-GAD67-Antibody-OTI3G9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70791-img0005.jpg "Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]")
Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]
Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GAD1/GAD67 mouse monoclonal antibody.![Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]](https://resources.rndsystems.com/images/products/GAD1-GAD67-Antibody-OTI3G9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70791-img0006.jpg "Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]")
Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]
Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GAD1/GAD67 mouse monoclonal antibody.![Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]](https://resources.rndsystems.com/images/products/GAD1-GAD67-Antibody-OTI3G9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70791-img0007.jpg "Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]")
Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]
Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GAD1/GAD67 mouse monoclonal antibody.![Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]](https://resources.rndsystems.com/images/products/GAD1-GAD67-Antibody-OTI3G9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70791-img0008.jpg "Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]")
Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]
Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791] - Staining of paraffin-embedded Human colon tissue using anti-GAD1/GAD67 mouse monoclonal antibody.![Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]](https://resources.rndsystems.com/images/products/GAD1-GAD67-Antibody-OTI3G9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70791-img0009.jpg "Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]")
Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]
Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791] - Staining of paraffin-embedded Human endometrium tissue using anti-GAD1/GAD67 mouse monoclonal antibody.![Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]](https://resources.rndsystems.com/images/products/GAD1-GAD67-Antibody-OTI3G9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70791-img0010.jpg "Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]")
Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]
Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791] - Staining of paraffin-embedded Human lung tissue using anti-GAD1/GAD67 mouse monoclonal antibody.![Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]](https://resources.rndsystems.com/images/products/GAD1-GAD67-Antibody-OTI3G9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70791-img0011.jpg "Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]")
Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]
Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791] - Staining of paraffin-embedded Human Ovary tissue using anti-GAD1/GAD67 mouse monoclonal antibody.![Immunoprecipitation: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]](https://resources.rndsystems.com/images/products/GAD1-GAD67-Antibody-OTI3G9-Azide-and-BSA-Free-Immunoprecipitation-NBP2-70791-img0013.jpg "Immunoprecipitation: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]")
Immunoprecipitation: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]
Immunoprecipitation: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791] - Negative control: IP without adding anti-GAD1 antibody.). For each experiment, 500ul of DDK tagged GAD1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-GAD1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.Applications for GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50
Immunohistochemistry
1:50
Immunoprecipitation
4ug/ml
Western Blot
1:1000-1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Calculators
Background: GAD1/GAD67
Long Name
Glutamate Decarboxylase 1 (Brain, 67kDa)
Alternate Names
GAD67
Gene Symbol
GAD1
Additional GAD1/GAD67 Products
Product Documents for GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Synaptic Neurotransmission Pathways: GABAergic Inhibition
