GAD1/GAD67 Antibody (OTI3G9)

Novus Biologicals | Catalog # NBP1-48272

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey, Zebrafish

Cited:

Human, Fish - Danio rerio (Zebrafish)

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI3G9
View all GAD1/GAD67 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human GAD1 (NP_000808) produced in HEK293T cell.

Reactivity Notes

Use in Zebrafish reported in scientific literature (PMID:33539794). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens glutamate decarboxylase 1 (brain, 67kDa) (GAD1), transcript variant GAD67.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

66.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GAD1/GAD67 Antibody (OTI3G9)

Western Blot: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272]

Western Blot: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272]

Western Blot: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GAD1/GAD67 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272]

Immunocytochemistry/ Immunofluorescence: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272]

Immunocytochemistry/Immunofluorescence: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Immunofluorescent staining of A549 cells using anti-GAD1/GAD67 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272]

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272]

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Staining of paraffin-embedded Human pancreas tissue using anti-GAD1/GAD67 mouse monoclonal antibody.
Western Blot: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272]

Western Blot: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272]

Western Blot: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GAD1/GAD67 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAD1/GAD67.
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272]

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272]

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GAD1/GAD67 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272]

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272]

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GAD1/GAD67 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272]

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272]

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GAD1/GAD67 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272]

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272]

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GAD1/GAD67 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272]

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272]

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Staining of paraffin-embedded Human colon tissue using anti-GAD1/GAD67 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272]

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272]

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Staining of paraffin-embedded Human endometrium tissue using anti-GAD1/GAD67 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272]

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272]

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Staining of paraffin-embedded Human lung tissue using anti-GAD1/GAD67 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272]

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272]

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Staining of paraffin-embedded Human Ovary tissue using anti-GAD1/GAD67 mouse monoclonal antibody.
Immunoprecipitation: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272]

Immunoprecipitation: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272]

Immunoprecipitation: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Negative control: IP without adding anti-GAD1 antibody.). For each experiment, 500ul of DDK tagged GAD1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-GAD1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Applications for GAD1/GAD67 Antibody (OTI3G9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Immunoprecipitation

4ug/ml

Peptide ELISA

Reported in scientific literature (PMID: 25358241)

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GAD1/GAD67

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.

Long Name

Glutamate Decarboxylase 1 (Brain, 67kDa)

Alternate Names

GAD67

Entrez Gene IDs

2571 (Human)

Gene Symbol

GAD1

Additional GAD1/GAD67 Products

Product Documents for GAD1/GAD67 Antibody (OTI3G9)

Certificate of Analysis

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Product Specific Notices for GAD1/GAD67 Antibody (OTI3G9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GAD1/GAD67 Antibody (OTI3G9)

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Protocols

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