GAD1/GAD67 Antibody

Novus Biologicals | Catalog # NBP1-06989

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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Bovine (100%), Canine (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all GAD1/GAD67 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-PDSPQRREKLHK corresponding to internal region according to NP_000808.2.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24291518)

Specificity

This antibody is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GAD1/GAD67 Antibody

Western Blot: GAD1/GAD67 Antibody [NBP1-06989]

Western Blot: GAD1/GAD67 Antibody [NBP1-06989]

Western Blot: GAD1/GAD67 Antibody [NBP1-06989] - Staining of Human Brain (Cerebellum) 35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for GAD1/GAD67 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:2000

Peptide ELISA

Detection limit 1:32000

Western Blot

0.5 - 1.5 ug/ml
Application Notes
WB: Approx. 65 kDa band observed in human brain (cerebral cortex) lysates (calculated MW of 66.9 kDa band according to NP_000808.2). IHC-F, ICC/IF usage has been reported in scientific literature (PMID: 24291518), however, results may vary from lot to lot.

Reviewed Applications

Read 1 review rated 1 using NBP1-06989 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GAD1/GAD67

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq]

Long Name

Glutamate Decarboxylase 1 (Brain, 67kDa)

Alternate Names

GAD67

Entrez Gene IDs

2571 (Human); 14415 (Mouse); 24379 (Rat)

Gene Symbol

GAD1

UniProt

Q99259

Additional GAD1/GAD67 Products

Product Documents for GAD1/GAD67 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for GAD1/GAD67 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GAD1/GAD67 Antibody

Customer Reviews for GAD1/GAD67 Antibody (1)

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  • GAD1/GAD67 Antibody
    Name: Filip Dybowski
    Application: Immunocytochemistry
    Sample Tested: Mouse brain 4% PFA fixed 40um sections
    Species: Mouse
    Verified Customer | Posted 06/08/2023
    NBP-06989 1:500 with AlexaFluor 405 secondary 1:1000. No signal present.
    Overnight incubation of free-floating sections in primary diluted in TBS-T (with NDS). Secondary incubated for 2 hours.
    GAD1/GAD67 Antibody NBP1-06989
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.

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