Glucagon Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-23954

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Glucagon Primary Antibodies »

Product Specifications

Immunogen

Glucagon antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human Glucagon.

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to human, chimpanzee, and bonobo.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Glucagon Antibody - BSA Free

Glucagon Antibody Immunoprecipitation: Glucagon Antibody [NBP3-23954] -

Immunoprecipitation: Glucagon Antibody [NBP3-23954] -

Immunoprecipitation: Glucagon Antibody [NBP3-23954] - Immunofluorescence of Glucagon Antibody. Cell Line: MCF7 cells. Fixative: 100% Methanol. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-Glucagon at 15µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG DyLight™488 at 5µL/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A). DAPI. (B). Anti-Glucagon + DyLight™488 secondary. (C). Merge A+B. (D). secondary only. Localization expected: Cytoplasm.
Glucagon Antibody ELISA: Glucagon Antibody [NBP3-23954] -

ELISA: Glucagon Antibody [NBP3-23954] -

ELISA: Glucagon Antibody [NBP3-23954] - ELISA Results of Glucagon Antibody. Each well was coated with 1µg of conjugate. The starting concentration of antibody in the dilution series was 5 µg/ml. The titer is 1:34,800 Glucagon - Free peptide [Green Line], 1:47200 Glucagon Standard - BSA conjugated [Blue Line], and 1:20,500 Glucagon - BSA conjugated [Purple Line]. Each point on the Y-axis represents a 3-fold dilution. 3% Fish Gel, HRP conjugated Goat anti-Rabbit IgG (H&L), and TMB substrate were used for detection.
Glucagon Antibody Immunocytochemistry/ Immunofluorescence: Glucagon Antibody [NBP3-23954] -

Immunocytochemistry/ Immunofluorescence: Glucagon Antibody [NBP3-23954] -

Immunocytochemistry/ Immunofluorescence: Glucagon Antibody [NBP3-23954] - Immunofluorescence of Glucagon Antibody. Cell Line: NIH/3T3 cells. Fixative: 100% Methanol. Permeabilization: Triton X-100. Primary Antibody: Anti-Glucagon at 15µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG DyLight™488 at 5µL/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A). DAPI. (B). Anti-Glucagon + DyLight™488 secondary. (C). Merge A+B. (D). secondary only. Localization expected: Cytoplasm.
Glucagon Antibody Immunocytochemistry/ Immunofluorescence: Glucagon Antibody [NBP3-23954] -

Immunocytochemistry/ Immunofluorescence: Glucagon Antibody [NBP3-23954] -

Immunocytochemistry/ Immunofluorescence: Glucagon Antibody [NBP3-23954] - Immunofluorescence of Glucagon Antibody. Cell Line: U20S cells. Fixative: 4% PFA. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-Glucagon at 15µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG DyLight™488 at 5µL/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A). DAPI. (B). Anti-Glucagon + DyLight™488 secondary. (C). Merge A+B. (D). secondary only. Localization expected: Cytoplasm.
Glucagon Antibody Immunocytochemistry/ Immunofluorescence: Glucagon Antibody [NBP3-23954] -

Immunocytochemistry/ Immunofluorescence: Glucagon Antibody [NBP3-23954] -

Immunocytochemistry/ Immunofluorescence: Glucagon Antibody [NBP3-23954] - Immunofluorescence of Glucagon Antibody. Cell Line: NIH/3T3 cells. Fixative: 4% PFA. Permeabilization: Triton X-100. Primary Antibody: Anti-Glucagon at 15µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG DyLight™488 at 5µL/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A). DAPI. (B). Anti-Glucagon + DyLight™488 secondary. (C). Merge A+B. (D). secondary only. Localization expected: Cytoplasm.
Glucagon Antibody Western Blot: Glucagon Antibody [NBP3-23954] -

Western Blot: Glucagon Antibody [NBP3-23954] -

Western Blot: Glucagon Antibody [NBP3-23954] - Western Blot of Glucagon Antibody. Lane 1: Opal Prestained Molecular Weight. Lane 2: COS-7 Lysate - reduced (20µg). Lane 3: BSA Conjugated Glucagon peptide - reduced (0.02µg).Lane 4: Insulin - reduced (0.05µg). Primary Antibody: Anti-Glucagon [Rabbit] Antibody at 1.0µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG (MX10) Peroxidase conjugated at 1:70,000 for 30mins at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 1hr at RT.Expected MW: ~21kDa. Observed MW: endogenous detection in COS-7 Lysate at ~35kDa. Glucagon peptide is detected at the MW of BSA. No cross-reactivity with insulin is observed.Exposure: 25 sec.
Glucagon Antibody Immunohistochemistry: Glucagon Antibody [NBP3-23954] -

Immunohistochemistry: Glucagon Antibody [NBP3-23954] -

Immunohistochemistry: Glucagon Antibody [NBP3-23954] - Immunohistochemistry results using Glucagon Antibody. Tissue: alpha cells in CD1 mouse pancreatic islets. Fixation: 4% paraformaldehyde. Antigen Retrieval: 10mM Sodium Citrate buffer for 10 mins at 95-100°C. Blocking: PBS, 1% ovalbumin, 0.3% Triton X-100. Primary Antibody: Anti-Glucagon at 1:100 overnight at RT. Secondary Antibody: Anti-Rabbit Alexa Fluor 488 at 1:500 for 1hr at RT. Original magnification 20x. Courtesy of Prof. Merighi, University of Turin.
Glucagon Antibody - BSA Free

Immunohistochemistry: Glucagon Antibody - BSA Free [NBP3-23954] -

Immunohistochemistry results using Glucagon Antibody - BSA Free. Tissue: alpha cells in CD1 mouse pancreatic islets. Fixation: 4% paraformaldehyde. Antigen Retrieval: 10mM Sodium Citrate buffer for 10 mins at 95-100°C. Blocking: PBS, 1% ovalbumin, 0.3% Triton X-100. Primary Antibody: Glucagon Antibody - BSA Free at 1:100 overnight at RT. Secondary Antibody: Anti-Rabbit Alexa Fluor 488 at 1:500 for 1hr at RT. Original magnification 20x. Courtesy of Prof. Merighi, University of Turin.
Glucagon Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Glucagon Antibody - BSA Free [NBP3-23954] -

Immunofluorescence of Glucagon Antibody - BSA Free. Cell Line: MCF7 cells. Fixative: 100% Methanol. Permeabilization: 0.3% Triton X-100. Primary Antibody: Glucagon Antibody - BSA Free at 15µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG DyLight™488 at 5µL/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A). DAPI. (B). Anti-Glucagon + DyLight™488 secondary. (C). Merge A+B. (D). secondary only. Localization expected: Cytoplasm.

Applications for Glucagon Antibody - BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Tested in ELISA, IF, and Western Blot. Although not tested, this antibody is suitable in immunohistochemistry. Expect a band approximately ~20.9 kDa corresponding to the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Formulation, Preparation, and Storage

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glucagon

The protein encoded by the Glucagon gene is actually a preproprotein that is cleaved into four distinct mature peptides. One ofthese, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulatingglycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signallingpathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promotenutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an activeenteroglucagon. (provided by RefSeq)

Alternate Names

GCG, GRPP

Gene Symbol

GCG

Additional Glucagon Products

Product Documents for Glucagon Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Glucagon Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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