Glut4 Antibody (3G10A3) - BSA Free

Flow Cytometry: Glut4 Antibody (3G10A3) - BSA Free [NBP2-22214]

Flow Cytometry: Glut4 Antibody (3G10A3) [NBP2-22214] - Flow cytometric analysis of HeLa cells using Glucose Transporter GLUT4 mouse mAb (green) and negative control (purple).

Western Blot: Glut4 Antibody (3G10A3)BSA Free [NBP2-22214]

Western Blot: Glut4 Antibody (3G10A3) [NBP2-22214] - Western blot analysis using Glucose Transporter Glut4 Antibody (3G10A3)mAb against HEK293 (1) and Glucose Transporter GLUT4 (AA: 224-353)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot: Glut4 Antibody (3G10A3)BSA Free [NBP2-22214]

Western Blot: Glut4 Antibody (3G10A3) [NBP2-22214] - human Glucose Transporter GLUT4 recombinant protein.

Immunocytochemistry/ Immunofluorescence: Glut4 Antibody (3G10A3) - BSA Free [NBP2-22214]

Immunocytochemistry/Immunofluorescence: Glut4 Antibody (3G10A3) [NBP2-22214] - Analysis of HeLa cells using Glucose Transporter GLUT4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry-Paraffin: Glut4 Antibody (3G10A3) - BSA Free [NBP2-22214]

Immunohistochemistry-Paraffin: Glut4 Antibody (3G10A3) [NBP2-22214] - Analysis of paraffin-embedded bladder cancer tissues using Glucose Transporter GLUT4 mouse mAb with DAB staining.

ELISA: Glut4 Antibody (3G10A3) - BSA Free [NBP2-22214]

ELISA: Glut4 Antibody (3G10A3) [NBP2-22214] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

Western Blot: Glut4 Antibody (3G10A3) - BSA Free [NBP2-22214] -

Supporting data 2 for Figure 1.(A-B) Body weight (A) and fasting glucose levels (B) of mice fed a HFD for 0, 1, 2, or 4 weeks. One-way ANOVA compared to 0 week HFD-fed mice, **p<0.01, ***p<0.001, ****p<0.0001. n=10 (C) ITT on mice fed a HFD for 0, 1, 2, 4 weeks, and mice fed 2 week HFD and 2 week ND. The mice were fasted for 6 hours and injected with insulin (0.75 U/kg body weight). One-way ANOVA, *p<0.05. n=8 (0 week ND), 10 (1 week HFD), 10 (2 week HFD), 10 (2 week HFD), and 6 (2 week HFD +2 week ND). (D–F) Immunoblots of vWAT(D), sWAT(E), and BAT (F) from mice fed a HFD for 0, 1, 2, or 4 weeks. Mice were fasted for 6 hours and injected with insulin (0.75 U/kg body weight). n=5. See Figure 1—figure supplement 2—source data 1.Figure 1—figure supplement 2—source data 1.Uncropped blots and source data for graphs for Figure 1—figure supplement 2.Uncropped blots and source data for graphs for Figure 1—figure supplement 2. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36920797), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Glut4 Antibody (3G10A3) - BSA Free [NBP2-22214] -

Supporting data 2 for Figure 1.(A-B) Body weight (A) and fasting glucose levels (B) of mice fed a HFD for 0, 1, 2, or 4 weeks. One-way ANOVA compared to 0 week HFD-fed mice, **p<0.01, ***p<0.001, ****p<0.0001. n=10 (C) ITT on mice fed a HFD for 0, 1, 2, 4 weeks, and mice fed 2 week HFD and 2 week ND. The mice were fasted for 6 hours and injected with insulin (0.75 U/kg body weight). One-way ANOVA, *p<0.05. n=8 (0 week ND), 10 (1 week HFD), 10 (2 week HFD), 10 (2 week HFD), and 6 (2 week HFD +2 week ND). (D–F) Immunoblots of vWAT(D), sWAT(E), and BAT (F) from mice fed a HFD for 0, 1, 2, or 4 weeks. Mice were fasted for 6 hours and injected with insulin (0.75 U/kg body weight). n=5. See Figure 1—figure supplement 2—source data 1.Figure 1—figure supplement 2—source data 1.Uncropped blots and source data for graphs for Figure 1—figure supplement 2.Uncropped blots and source data for graphs for Figure 1—figure supplement 2. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36920797), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Glut4 Antibody (3G10A3) - BSA Free [NBP2-22214] -

Supporting data 2 for Figure 1.(A-B) Body weight (A) and fasting glucose levels (B) of mice fed a HFD for 0, 1, 2, or 4 weeks. One-way ANOVA compared to 0 week HFD-fed mice, **p<0.01, ***p<0.001, ****p<0.0001. n=10 (C) ITT on mice fed a HFD for 0, 1, 2, 4 weeks, and mice fed 2 week HFD and 2 week ND. The mice were fasted for 6 hours and injected with insulin (0.75 U/kg body weight). One-way ANOVA, *p<0.05. n=8 (0 week ND), 10 (1 week HFD), 10 (2 week HFD), 10 (2 week HFD), and 6 (2 week HFD +2 week ND). (D–F) Immunoblots of vWAT(D), sWAT(E), and BAT (F) from mice fed a HFD for 0, 1, 2, or 4 weeks. Mice were fasted for 6 hours and injected with insulin (0.75 U/kg body weight). n=5. See Figure 1—figure supplement 2—source data 1.Figure 1—figure supplement 2—source data 1.Uncropped blots and source data for graphs for Figure 1—figure supplement 2.Uncropped blots and source data for graphs for Figure 1—figure supplement 2. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36920797), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Glut4 Antibody (3G10A3) - BSA Free [NBP2-22214] -

mTORC2 in adipose tissue is required for cold‐induced glucose uptake and glycolysis2‐deoxyglucose‐6‐phosphate (2DG6P) accumulation in BAT of AdRiKO and control mice housed at 22 or at 4C for 4 h (n = 6/group).Extracellular acidification rate (ECAR) of BAT explants from AdRiKO and control mice housed at 22 or at 4C for 4 h (n = 7/group).Immunoblot analysis of BAT from AdRiKO and control mice housed at 22 or at 4C for 8 h for the indicated proteins (n = 6/group, each lane represents a mix of 3 mice).Immunoblot analysis of isolated plasma membranes from BAT of AdRiKO and control mice housed at 22 or at 4C for 8 h for the indicated proteins (n = 6/group, each lane represents a mix of 3 mice).Immunoblot analysis of mitochondrial and cytosolic fractions from BAT of AdRiKO and control mice housed at 22 or at 4C for 4 h for the indicated proteins (n = 6/group, each lane represents a mix of 3 mice).Cytosolic hexokinase activity in BAT of AdRiKO and control mice housed at 22 or at 4C for 4 h [n = 5 (control 22C), n = 5 (AdRiKO 22C), n = 7 (control 4C), n = 7 (AdRiKO 4C)].Mitochondrial hexokinase activity in BAT of AdRiKO and control mice housed at 22 or at 4C for 4 h [n = 5 (control 22C), n = 5 (AdRiKO 22C), n = 7 (control 4C), n = 7 (AdRiKO 4C)].Data information: Data represent mean +/- SEM. Statistically significant differences between AdRiKO and control mice were determined with unpaired Student's t‐test and are indicated with asterisks (*P < 0.05). Statistically significant differences between temperatures were determined with unpaired Student's t‐test and are indicated with a number sign (#P < 0.05; ##P < 0.01; ###P < 0.001). The exact P‐value for each significant difference can be found in Appendix Table S2. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26772600), licensed under a CC-BY license. Not internally tested by Novus Biologicals.