GMP Cloudz Human T Cell Activation Kit
GMP Cloudz Human T Cell Activation Kit Summary
Key Benefits
• > 50-fold expansion of T cells from PBMCs cells in 10 days
• Dissolvable CD3/28 microspheres enable T cell expansions
• Optimized for use with the ExCellerate™ Human T Cell Media
• Supports T cell expansion from PBMCs or purified CD3+ PBMCs
• Optimized for use with R&D Systems cytokines and media
Why Expand T Cells ex vivo Using GMP Cloudz Human T Cell Activation Kit?
T Lymphocytes (T Cells) can be used as a powerful immunotherapy to fight against cancer and other diseases. GMP Cloudz Human T cell Activation Kit allows for a robust expansion platform utilizing dissolvable CD3/28 microspheres that can easily be removed from expanded cells. GMP Cloudz Human T Cell Activation kit supports the expansion of human T cell lines or T cell clones and the stimulation of human peripheral blood lymphocytes.
Versatility of GMP Cloudz Human T Cell Activation Kit
GMP Cloudz Human T Cell Activation Kit is optimized for T cell expansion when used in combination with
Kit Contents
• 2 x 12 mL of GMP-Grade Cloudz CD3/CD28
• 2 x 100 mL of GMP-Grade Cloudz 6X Release Buffer
Each kit contains enough reagents to activate 4x108 CD3+ T Cells.
Specifications
Limitations
For research use only. Not for diagnositic use.
Product Datasheets
Data Example
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Expansion of Human T Cells using GMP Cloudz™ Human T Cell Activation Kit Primary human CD3+ cells were activated with GMP Cloudz™ Human T-Cell Activation Kit and cultured for 9 days in ExCellerate™ T Cell Expansion Media (R&D Systems, Catalog # CCM030) and 20 ng/mL IL-2 (R&D systems, Catalog # 202-GMP). A. Cell counts were performed to determine fold expansion compared to the Day 0 seeding density (0.25 x 106 cells/mL).
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Viability of Human T Cells using Cloudz™ Human T Cell Activation Kit. Primary human CD3+ cells were activated with GMP Cloudz™ Human T-Cell Activation Kit and cultured for 9 days in ExCellerate™ T Cell Expansion Media (R&D Systems, Catalog # CCM030) and 20 ng/mL IL-2 (R&D systems, Catalog # 202-GMP). Cell counts were performed to determine percent viability on days 5, 7, and 9 of the experiment compared to the Day 0 seeding density (0.25 x 106 cells/mL).
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Phenotypic analysis of CD25/CD69 Markers Primary human CD3+ cells were A. activated with GMP Cloudz™ Human T Cell Activation Kit and cultured for 48 hours in ExCellerate™ T Cell Expansion Media (R&D Systems, Catalog # CCM030) and 20 ng/mL IL-2 (R&D systems, Catalog #202-GMP). B. The negative control was performed without adding Cloudz T Cell microspheres. Cells were fluorescently stained with using CD25-PE (R&D Systems, Catalog # FAB1020P) and CD69-a488 (R&D Systems, Catalog # FAB23591G) and analyzed via flow cytometry. Dead cells and debris were excluded from the analysis on scatter signals and viability dye. Cells that were treated with GMP Cloudz™ Human T Cell Activation Kit showed an activation signal 48 hours after reagent addition.
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