GPER/GPR30 Antibody - BSA Free
Novus Biologicals | Catalog # NLS4271
![Western Blot: GPER/GPR30 AntibodyBSA Free [NLS4271]](https://beta-resources.rndsystems.com/images/products/GPER-GPR30-Antibody-Western-Blot-NLS4271-img0004.jpg "Western Blot: GPER/GPR30 AntibodyBSA Free [NLS4271]")
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Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Monkey
Cited:
Human, Mouse, Rat
Predicted:
Canine (94%), Hamster (94%), Primate (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Cited:
Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Synthetic 16 amino acid peptide from 3rd extracellular domain of human GPER/GPR30.
Epitope
3rd extracellular domain
Reactivity Notes
Predicted cross-reactivity based on sequence identity: Gorilla (100%), Marmoset (100%), Bat (88%), Equine (88%), Rabbit (88%). Mouse reactivity reported in (PMID: 23273253). Rat reactivity reported from a verified customer review.
Localization
Endoplasmic reticulum - multi-pass membrane protein.
Specificity
Human GPR30. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Product can be stored undiluted at 4C for up to 1 month.
Scientific Data Images for GPER/GPR30 Antibody - BSA Free
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS4271]](https://beta-resources.rndsystems.com/images/products/GPER-GPR30-Antibody-Immunohistochemistry-Paraffin-NLS4271-img0003.jpg "Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS4271]")
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS4271]
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS4271] - Analysis of anti-GPR30 antibody with human brain, hippocampus.![Western Blot: GPER/GPR30 AntibodyBSA Free [NLS4271]](https://beta-resources.rndsystems.com/images/products/GPER-GPR30-Antibody-Western-Blot-NLS4271-img0002.jpg "Western Blot: GPER/GPR30 AntibodyBSA Free [NLS4271]")
Western Blot: GPER/GPR30 AntibodyBSA Free [NLS4271]
Western Blot: GPER/GPR30 Antibody [NLS4271] - Analysis of anti-GPR30 antibody with rat hypothalamus 5-10ug protein loaded per lane. Image courtesy provided by Carrie McAllister.
Western Blot: GPER/GPR30 Antibody - BSA Free [NLS4271] -
Direct association of dopamine transporter and estrogen receptors on the plasma membrane vs. in total protein. Co-immunoprecipitation of dopamine transporter after 9 min 10-9 M E2 treatment and subsequent probing with antibodies for DAT, ER alpha, ER beta, and GPR30. This is a representative immunoblot of 3 experiments
Western Blot: GPER/GPR30 Antibody - BSA Free [NLS4271] -
Western Blot: GPER/GPR30 Antibody - BSA Free [NLS4271] - GPER/GPR30 expression in normal lung cell lines & lung adenocarcinoma cell lines. A) The expression of GPER was determined by realtime quantitative PCR in each of the normal lung cell lines (open bars), lung adenocarcinoma cell lines (grey bars), & MCF-7 human breast cancer cells (black bar). Values are the average of 4–6 biological replicates ± SEM. B-D) Representative western blot analysis of GPER/GPR30 expression in the indicted cell lines. 30 μg of whole cell extract (WCE) were separated on 10% SDS PAGE gels with MW marker migration indicated at the left of each blot. The diagrams at the top of B-C, & D indicate the region of GPER recognized by the 3 different polyclonal antibodies used: B) Novus NBP-1-31239, C) Novus NLS4271; D) Santa Cruz sc-4854-R. The MW of GPR30 is estimated to be 42 kDa, but higher MW sizes have been reported due to glycosylation & interaction with other proteins. Bands are identified as glycosylated & nonglycosylated based on reports cited in the text, but could include interaction with other proteins. For each GPER western, the membrane was stripped & reprobed for beta -actin as a loading control. Quantitation of GPER was evaluated by summing all immunoreactive bands & dividing by beta -actin, then normalizing to HBEC2-KT in each blot. Panel E is a summary of all westerns (not all westerns are shown) with each antibody. For Westerns with NBP1-31239 & sc-48524, values are the mean ± SEM from 3 separate blots using different WCE. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23273253), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for GPER/GPR30 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
6.4 ug/ml
Western Blot
1:1000
Application Notes
Antibody does not work in Western Blot for mouse samples. IP usage is reported in scientific literature (PMID: 19531209). ELISA usage is reported in scientific literature (PMID: 18489713). Use in ICC/IF reported in scientific literature (PMID: 21427217)..
Reviewed Applications
Read 1 review rated 4 using NLS4271 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Background: GPER/GPR30
Long Name
G Protein-coupled Estrogen Receptor 1
Alternate Names
CEPR, CMKRL2, DRY12, FEG-1, GPCR-BR, GPR30, LERGU2, LyGPR
Entrez Gene IDs
2852 (Human)
Gene Symbol
GPER1
UniProt
Additional GPER/GPR30 Products
Product Documents for GPER/GPR30 Antibody - BSA Free
Product Specific Notices for GPER/GPR30 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for GPER/GPR30 Antibody - BSA Free
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Application: Western BlotSample Tested: rat hypothalamusSpecies: RatVerified Customer | Posted 01/24/2013
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for GPER/GPR30 Antibody - BSA Free
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A: The expected molecular weight of GPR30 is 42.3, however, because this protein has at least 3 different glycosylation sites, you should not be surprised if you see a band at higher position in your samples.
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